One-step type embryo culture liquid

An embryo culture solution and embryo culture technology, applied in the field of one-step embryo culture solution, can solve the problems of harmful decomposition products and achieve the effects of protecting cells, promoting safety, and inhibiting the decomposition and utilization of harmful products

Inactive Publication Date: 2019-11-15
成都艾伟孚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole culture medium series process retains these important active ingre

Method used

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  • One-step type embryo culture liquid
  • One-step type embryo culture liquid
  • One-step type embryo culture liquid

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1

[0033] One-step embryo culture medium

[0034] Material name Formula content Sodium chloride 99.937mmol / L Potassium chloride 5.531mmol / L Magnesium Sulfate 1.0mmol / L glucose 1.0mmol / L Gentamicin 9.5mg / L Ethylenediaminetetraacetic acid 0.01mmol / L Sodium pyruvate 10.51mmol / L Alanyl Glutamine 27.49mmol / L Taurine 0.48mmol / L D-Sorbitol 0.23mmol / L Calcium chloride 1.8mmol / L Sodium dihydrogen phosphate 0.15mmol / L Potassium Dihydrogen Phosphate 0.15mmol / L Sodium bicarbonate 0.64mmol / L (±)-α-Lipoic acid 0.078mmol / L Acetyl L-carnitine hydrochloride or acetyl L-carnitine 0.078mmol / L N-acetyl-L-cysteine 0.785mmol / L 4-hydroxyethylpiperazine ethanesulfonic acid HEPES 9.225mmol / L 3-(N-morpholino) propanesulfonic acid sodium salt MOPS-Na 9.165mmol / L Essential amino acids 1.6mmol / L Non-essential amino acid 0.9mmol / L Serum albumin 0.5g / L Serum replacement solution 0.95%, v / v

Example Embodiment

[0035] Example 2

[0036] One-step embryo culture medium

[0037] Material name Formula content Sodium chloride 89.937mmol / L Potassium chloride 5.013mmol / L Magnesium Sulfate 0.898mmol / L glucose 0.899mmol / L Gentamicin 5.0mg / L Ethylenediaminetetraacetic acid 0.009mmol / L Sodium pyruvate 9.459mmol / L Alanyl Glutamine 24.741mmol / L Taurine 0.432mmol / L D-Sorbitol 0.207mmol / L Calcium chloride 1.621mmol / L Sodium dihydrogen phosphate 0.135mmol / L Potassium Dihydrogen Phosphate 0.135mmol / L Sodium bicarbonate 0.576mmol / L (±)-α-Lipoic acid 0.055mmol / L Acetyl L-carnitine hydrochloride or acetyl L-carnitine 0.044mmol / L N-acetyl-L-cysteine 0.702mmol / L 4-hydroxyethylpiperazine ethanesulfonic acid HEPES 8.125mmol / L 3-(N-morpholino) propanesulfonic acid sodium salt MOPS-Na 8.125mmol / L Essential amino acids 0.7mmol / L Non-essential amino acid 0.5mmol / L Serum albumin 0.45g / L Serum replacement solution 0.85%, v / v

Example Embodiment

[0038] Example 3

[0039] One-step embryo culture medium

[0040]

[0041]

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PUM

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Abstract

The invention discloses a one-step type embryo culture liquid. The one-step type embryo culture liquid comprises substances of (+/-)-alpha-lipoic acid, acetyl L-carnitine or acetyl L-carnitine hydrochloride and insulin, transferrin, selenium, Fetuin-A and platelet derived growth factor (PDGF) which play an important role in embryo culture and development. The one-step type embryo culture solutionprepared through the substances retain favorable components secreted into a culture solution in the embryo culture and development process, the effects of protecting and promoting the growth and development of cells and inhibiting decomposition and utilization of harmful products by the cells, and the problem is avoided that the blastocyst formation rate caused by mechanical damage likely to be brought to embryos and a stress reaction caused by changes of the culture environment in the transplanting process is low.

Description

technical field [0001] The invention belongs to the field of embryo cell culture, in particular to a one-step embryo culture solution. Background technique [0002] IVF, in vitro fertilization (in vitro fertilization): also known as test-tube baby, refers to taking out eggs and sperm respectively, placing them in test tubes to fertilize them, and then transplanting the embryo precursor - fertilized eggs back into the mother's uterus to develop into fetuses . [0003] IVF culture is usually divided into two processes, that is, two culture medium series: whole process (single / one-step culture medium series) and split process (double culture medium series). The use of double culture solution series processes provides different culture solution components for the early and late stages of embryos, but also loses important effective components secreted by embryos into the culture solution, such as embryonic nutritional factors. The whole culture medium series process retains the...

Claims

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Application Information

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IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/25C12N2500/30C12N2501/135C12N2501/998
Inventor 余裕炉叶林刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
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