Eye stem cell culture fluid

A stem cell culture, eye technology, applied in cell culture active agent, culture process, tissue culture, etc., can solve the problems of donor rejection surgery, failure, limited number of donors, etc.

Inactive Publication Date: 2019-11-15
杨慧慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Corneal donors generally come from corneal donors. On the one hand, the number of donors is limited. On the other hand, there is still an immune reaction after allogeneic corneal transplantation, and even donor rejection occurs, resulting in surgical failure.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The ocular stem cell culture solution of the present invention includes the following components: low-sugar MEM medium, fetal calf serum, non-essential amino acids, vascular endothelial growth factor, growth hormone release inhibitory factor and platelet-derived proliferation factor.

[0031] Wherein, the low-sugar type MEM medium described in every 1L comprises the following components:

[0032] Fetal bovine serum 0.8L

[0033] Non-essential amino acids 5g

[0034] Vascular Endothelial Growth Factor 0.5mg

[0035] Somatostatin 0.4mg

[0036] Platelet-derived growth factor 2mg.

[0037] Wherein, the preparation method of low-sugar type MEM medium is as follows:

[0038] Preparation before S1 configuration: configure the newly prepared milliwater for the medium, then clean the vessel for preparing the medium, and rinse it twice with milliwater;

[0039] S2. Dissolving medium: Take milliwater with a final volume fraction of 5%, add it to a 1L glass, pour the dry powd...

Embodiment 2

[0048] The ocular stem cell culture solution of the present invention includes the following components: low-sugar MEM medium, fetal calf serum, non-essential amino acids, vascular endothelial growth factor, growth hormone release inhibitory factor and platelet-derived proliferation factor.

[0049] Wherein, the low-sugar type MEM medium described in every 1L comprises the following components:

[0050] Fetal bovine serum 1L

[0051]Non-essential amino acids 17.5g

[0052] Vascular Endothelial Growth Factor 1.5mg

[0053] Somatostatin 0.3mg

[0054] Platelet-derived proliferation factor 1.25mg.

[0055] Wherein, the preparation method of low-sugar type MEM medium is as follows:

[0056] Preparation before S1 configuration: configure the newly prepared milliwater for the medium, then clean the vessel for preparing the medium, and rinse it twice with milliwater;

[0057] S2. Dissolving medium: Take milliwater with a final volume fraction of 5%, add it to a 1L glass, pour th...

Embodiment 3

[0066] The ocular stem cell culture solution of the present invention includes the following components: low-sugar MEM medium, fetal calf serum, non-essential amino acids, vascular endothelial growth factor, growth hormone release inhibitory factor and platelet-derived proliferation factor.

[0067] Wherein, the low-sugar type MEM medium described in every 1L comprises the following components:

[0068] Fetal bovine serum 1.2L

[0069] Non-essential amino acids 30g

[0070] Vascular Endothelial Growth Factor 2.5mg

[0071] Somatostatin 0.25mg

[0072] Platelet-derived proliferation factor 0.5mg.

[0073] Wherein, the preparation method of low-sugar type MEM medium is as follows:

[0074] Preparation before S1 configuration: configure the newly prepared milliwater for the medium, then clean the vessel for preparing the medium, and rinse it twice with milliwater;

[0075] S2. Dissolving medium: Take milliwater with a final volume fraction of 5%, add it to a 1L glass, pour t...

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Abstract

The invention provides eye stem cell culture fluid, and belongs to the technical field of biology. The eye stem cell culture fluid is prepared through mixing low-sugar type MEM culture mediums, fetalcalf serum, non-essential amino acids, blood vessel endothelium growth factors, growth hormone release inhibiting factors and platelet-derived proliferation factors. The eye stem cell culture fluid can enable eye stem cells to have high proliferation capacity and fibroblast disintegrating potential, and regeneration of eye cells is facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an ocular stem cell culture solution. Background technique [0002] Corneal trauma is a common disease in ophthalmology clinics, and it can even lead to blindness in severe cases. The normal corneal epithelialization process is completed by the regeneration and differentiation of stem cells in the basal layer of the limbal epithelium. Corneal injury leads to corneal epithelial defect, and the secondary inflammatory reaction will affect the proliferation and migration of stem cells, form pannus, aggravate corneal edema and scarring, corneal turbidity affects light transmission, and even cause corneal ulcers. Corneal transplantation is still the only reliable and effective treatment for clinical corneal blindness. Corneal donors generally come from corneal donors. On the one hand, the number of donors is limited. On the other hand, there is still an immune reaction after allogeneic k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079C12N5/0797
CPCC12N5/0621C12N5/0623C12N2500/32C12N2501/135C12N2501/165C12N2501/36
Inventor 杨慧慧
Owner 杨慧慧
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