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Molecular marker for diagnosis of diabetes and application thereof

A molecular marker, a technology for diagnosing diabetes, applied in the field of molecular markers for diabetes diagnosis

Pending Publication Date: 2019-11-15
淄博市中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the diagnosis of diabetes mainly relies on clinical examination and various auxiliary technologies. Although medical technology has been highly developed compared with the past, there are still no reliable biomarkers in clinical practice for risk prediction and early diagnosis of diabetes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A molecular marker for diabetes diagnosis, comprising a test kit and diagnostic primers, characterized in that the kit is provided with a chip inside, the chip includes a gene chip, gene primers and molecular markers, and the diagnostic primers include A forward primer and a reverse primer, the forward primer and the reverse primer are both set to RNA molecular sequences; the molecular markers are set to the non-coding RNA molecular markers, and the non-coding RNA molecular markers are has- miR-802 and has-lncRNA-ROIT, the molecular markers were used as positive controls.

[0022] The gene chip includes a solid phase carrier and detection probes, the detection probes are fixed on the surface of the solid phase carrier, and the detection probes are oligonucleotide probes.

[0023] The gene primer is set as a primer reagent or probe for molecular markers, and a citric acid detection reagent is arranged inside the reagent or probe.

[0024] An application for diabetes dia...

Embodiment 3

[0035] A kind of molecular marker for the diagnosis of diabetes and its application provided by the embodiment and the second embodiment are clinically tested:

[0036] A normal control group was selected: 30 cases, 17 males and 13 females, with an average age of 54, 6±9, 6 years old, no history of infectious diseases, thrombosis, bleeding, and liver and kidney diseases, and no anticoagulants and contraceptives were used;

[0037] Type 2 DM group: 60 cases, 32 males and 28 females, with an average age of 55, 8±9, and 6 years old, diagnosed in line with the diagnostic criteria for diabetes established by WHO, with an average blood sugar concentration of 14, 16±5, 96mmol / L . Among them, 30 patients with no clinical complication of heart, brain, kidney and fundus vascular lesions belonged to the type 2 DM without clinical complication group;

[0038] 30 patients with one or more complications of heart, brain, kidney and fundus vascular lesions clinically belonged to the type 2 D...

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PUM

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Abstract

The invention belongs to the technical field of biology, and particularly to a molecular marker for diagnosis of diabetes and application thereof. For the problem that currently, no reliable biomarkercan perform risk prediction and early diagnosis on the diabetes, the following solution is proposed that the molecular marker comprises a kit and diagnostic primers, wherein the kit is internally provided with a chip, the chip comprises a gene chip, a gene primer and a molecular marker, the diagnostic primers comprise the forward primer and the reverse primer, and the forward primer and the reverse primer are both set to have RNA molecule sequences. Trough the detection of items related to a thrombus molecular marker, not only is further knowing of complications of DM vascular injuries and the pathogenesis of thrombosis facilitated, but also a basis is provided for using anticoagulant antiplatelet drugs for treatment, and the detection of molecules of type 2 diabetes patients has important clinical significance for the early prevention or intervention of thrombogenesis and vasculopathy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for diabetes diagnosis and its application. Background technique [0002] Diabetes mellitus is a metabolic disorder characterized by high and chronic blood glucose (referred to as blood sugar) levels caused by absolute or relative lack of insulin. It is one of the most important non-communicable diseases currently threatening global human health. According to statistics from the Union, the number of diabetic patients in the world has reached 370 million in 2011, 80% of which are in developing countries. It is estimated that there will be nearly 550 million diabetic patients in the world by 2030. In 2011, a total of 4.6 million people died of diabetes worldwide, and the global medical cost of diabetes reached 465 billion US dollars in that year. Among them, the rapid growth of diabetes in China and other developing countries has brought a heavy burden to the social...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 相新新刘永亮
Owner 淄博市中心医院