Preparation method of tumor vaccine and tumor vaccine prepared by using the method

A tumor vaccine and tumor antigen technology, applied in the field of cellular immunotherapy, can solve the problems of low immune response level and high adjuvant dependence of tumor vaccines, achieve strong immune stimulating activity, improve screening accuracy, and improve efficiency
CN110464840APending Publication Date: 2019-11-19北京微九九科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京微九九科技有限公司
Publication Date
2019-11-19

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Abstract

The invention discloses a preparation method of a tumor vaccine. The method includes: cloning a gene sequence of a tumor antigen obtained by screening into a virus expression vector to prepare a recombinant virus to obtain the tumor vaccine; inserting a signal peptide sequence shown in SEQ ID No. 1 or a sequence having a homology of more than 80% with the signal peptide sequence to 5 'end of the gene sequence of the first tumor antigen, and inserting an MHC transport signal sequence shown in SEQ ID No. 2 or a sequence having more than 80% homology with the MHC transport signal sequence to 3 'end of the gene sequence of the last tumor antigen; wherein the virus expression vector is an adenovirus, a retrovirus or a lentivirus, preferably the adenovirus. According to the method, the tumor antigen is screened by a specific method, the virus vector, particularly the adenovirus vector, is used for expressing and preparing the vaccine for the tumor antigen. The vaccine has strong immune stimulation activity and can reduce the requirement on immune adjuvants.
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Description

technical field

[0001] The invention relates to the technical field of cellular immunotherapy, in particular to a method for preparing a tumor vaccine and a tumor vaccine prepared by using the method. Background technique

[0002] Tumor cells are characterized by genome instability and rapid accumulation of somatic mutations. Genome sequencing shows that tumor cells are highly heterogeneous, with hundreds or thousands of somatic mutations in cancer patients, and the types of mutations are also diverse , including point mutations, insertion-deletion mutations, gene amplification, gene rearrangement, etc. Some mutations can cause mutations in the protein encoded by the gene, and the mutated protein can lead to uncontrolled cell proliferation, leading to tumorigenesis.

[0003] Cancer vaccines have always been a research hotspot in the field of tumor treatment, but there are many difficulties in the research process, such as the selection of antigens, adjuvants, and delivery m...

Claims

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