Hormone and temperature-combined regulation method for emergence of aphidius gifuensis ashmaed from stiff aphids

A technology of Aphids persicae and Aphids aphids, applied in the field of regulation of hormone and temperature on the emergence of Aphis persicum and Aphis persicae

Active Publication Date: 2019-11-22
中国烟草总公司福建省公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether different types and concentrations of juvenile hormones have the same physiological effects in different insects remains to be elucidated.

Method used

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  • Hormone and temperature-combined regulation method for emergence of aphidius gifuensis ashmaed from stiff aphids
  • Hormone and temperature-combined regulation method for emergence of aphidius gifuensis ashmaed from stiff aphids
  • Hormone and temperature-combined regulation method for emergence of aphidius gifuensis ashmaed from stiff aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Effects of Different Temperatures and Different Juvenile Hormone Combinations on Emerging Aphids

[0020] Tobacco variety: flue-cured tobacco K326.

[0021] Source of Aphids persicae: Collected from Nanping Tobacco Company and Shaowu Company's artificial breeding base of A.sia persicae, and tested after 2-3 generations indoors.

[0022] Reagents: ZR-512 (methoprene) (purity 97.00%, produced by Dr. Ehrenstorfer GmbH, Germany), ZR-777 (methoprene) (purity 96.80%, produced by Dr. Ehrenstorfer GmbH, Germany), acetone ( Purity 99.5%, produced by Xilong Science Co., Ltd.).

[0023] Instrument materials: intelligent artificial climate box (MRC-250B-LED, produced by Ningbo Plant Instrument Co., Ltd.), insect-proof net cover (120 mesh), plastic tank with cover (diameter×height=9.3cm×8.3cm), filter paper, Absorbent cotton, distilled water, centrifuge tube, brush, Erlenmeyer flask, beaker.

[0024] Preparation of test solution: Dissolve 10 mg of juvenile hormone analo...

Embodiment 2

[0039] A method for regulating and controlling the emergence of Aphids persicae by hormones and temperature is as follows: immerse A.spermis persicum in the ZR-512 juvenile hormone analogue reagent with a concentration of 1000 ng / μl for 5 seconds, then take it out with The filter paper was blotted to dry the residual reagent, and placed in a plastic jar with a cover; then placed in a relative humidity of 75%, a photoperiod of 14L: 10D, and a temperature of 26°C for cultivation, and observed and recorded the emergence of aphids.

Embodiment 3

[0041] A control method of hormone and temperature on the emergence of Aphids persicae Aphids is as follows: immerse Aphids persicum in the ZR-512 juvenile hormone analogue reagent with a concentration of 1000ng / μl for 6 seconds, then take it out with The filter paper was blotted to dry the residual reagent, and placed in a plastic jar with a cover; then placed in a relative humidity of 75%, photoperiod 14L: 10D, and cultured at a temperature of 10°C, and observed and recorded the bee emergence of the aphid.

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PUM

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Abstract

The invention provides a regulation method for the growth and development of aphidius gifuensis ashmaed and belongs to the field of biotechnology. The method adopts 8-5000 ng / microliter ZR-512 or 8-1,000 ng / microliter ZR-777 liquid for immersion treatment of stiff aphids of the aphidius gifuensis ashmaed, so that the emergence time of the aphidius gifuensis ashmaed is prolonged by 8-56 hours, andmeanwhile the life span and the parasitism rate of the aphidius gifuensis ashmaed after emergence are not affected. The method is simple, easy to implement, low in cost and significant in effect, canbe directly used for artificial reproduction of the aphidius gifuensis ashmaed, is used for temporarily saving the stiff aphids of the aphidius gifuensis ashmaed, is applied to the field for preventing and controlling the aphidius gifuensis ashmaed, provides sufficient and uniform stiff aphids of the aphidius gifuensis ashmaed for the prevention and control of the aphidius gifuensis ashmaed, reduces aphids and spread diseases thereof, and reduces tobacco leaf loss; by using the method, the use of chemical pesticides can be effectively reduced, environmental pollution can be reduced, and the safety of tobacco leaves and other plants can be improved.

Description

technical field [0001] The invention belongs to the technical field of plant pest control, and in particular relates to a method for regulating and controlling the emergence of aphids by hormones and temperature on the emergence of aphids from the aphid. Background technique [0002] Aphid [ Myzus persicae (Sulzer)] also known as peach aphid, belongs to Homoptera, Aphididae. The insect has a wide range of host plants, mainly Brassicaceae, Solanaceae, Rosaceae, Fabaceae, Chenopodiaceae and other plants. It uses its mouth needle to suck the juice of eating plants, which is very harmful. [0003] In the control of tobacco aphids, the extensive, large-scale and long-term use of chemical pesticides has caused the "3R (Residue, Resistance, Resurgence)" problem in tobacco leaf production to become more and more serious, making it more and more difficult to prevent and control aphids and aphid-borne viral diseases in tobacco leaf production . Aphids aphids ( Aphidius gifuensis...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 顾钢赖荣泉白晶晶周挺舒静
Owner 中国烟草总公司福建省公司
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