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A method for regulating and controlling the emergence of parasitic aphids from Aphids persicae by hormones and temperature

A technology of Braconia serrata and Aphid spp

Active Publication Date: 2021-08-10
中国烟草总公司福建省公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether different types and concentrations of juvenile hormones have the same physiological effects in different insects remains to be elucidated.

Method used

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  • A method for regulating and controlling the emergence of parasitic aphids from Aphids persicae by hormones and temperature
  • A method for regulating and controlling the emergence of parasitic aphids from Aphids persicae by hormones and temperature
  • A method for regulating and controlling the emergence of parasitic aphids from Aphids persicae by hormones and temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Effects of Different Temperatures and Different Juvenile Hormone Combinations on Emerging Aphids

[0020] Tobacco variety: flue-cured tobacco K326.

[0021] Source of Aphids persicae: Collected from Nanping Tobacco Company and Shaowu Company's artificial breeding base of A.sia persicae, and tested after 2-3 generations indoors.

[0022] Reagents: ZR-512 (methoprene) (purity 97.00%, produced by Dr. Ehrenstorfer GmbH, Germany), ZR-777 (methoprene) (purity 96.80%, produced by Dr. Ehrenstorfer GmbH, Germany), acetone ( Purity 99.5%, produced by Xilong Science Co., Ltd.).

[0023] Instrument materials: intelligent artificial climate box (MRC-250B-LED, produced by Ningbo Plant Instrument Co., Ltd.), insect-proof net cover (120 mesh), plastic tank with cover (diameter×height=9.3cm×8.3cm), filter paper, Absorbent cotton, distilled water, centrifuge tube, brush, Erlenmeyer flask, beaker.

[0024] Preparation of test solution: Dissolve 10 mg of juvenile hormone analo...

Embodiment 2

[0039] A method for regulating and controlling the emergence of Aphids persicae by hormones and temperature is as follows: immerse A.spermis persicum in the ZR-512 juvenile hormone analogue reagent with a concentration of 1000 ng / μl for 5 seconds, then take it out with The filter paper was blotted to dry the residual reagent, and placed in a plastic jar with a cover; then placed in a relative humidity of 75%, a photoperiod of 14L: 10D, and a temperature of 26°C for cultivation, and observed and recorded the emergence of aphids.

Embodiment 3

[0041] A control method of hormone and temperature on the emergence of Aphids persicae Aphids is as follows: immerse Aphids persicum in the ZR-512 juvenile hormone analogue reagent with a concentration of 1000ng / μl for 6 seconds, then take it out with The filter paper was blotted to dry the residual reagent, and placed in a plastic jar with a cover; then placed in a relative humidity of 75%, photoperiod 14L: 10D, and cultured at a temperature of 10°C, and observed and recorded the bee emergence of the aphid.

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Abstract

The invention provides a method for regulating and controlling the growth and development of Aphidia persicae, which belongs to the field of biotechnology. In this method, ZR-512 with a concentration of 8-5000ng / μl or ZR-777 with a concentration of 8-1000ng / μl is used to treat the dead aphids of Aphids persicae, so that the bee-out time of A. It does not affect the adult bee lifespan and parasitism rate of Aphids aphidis. The method of the present invention is simple and easy to implement, low in cost, and remarkable in effect, and can be directly used for artificial propagation of Aphids persicae, temporarily preserves the dead aphids of A. Aphids aphids prevent and control aphids, reduce aphids and the diseases they spread, and reduce the loss of tobacco leaves; it can effectively reduce the use of chemical pesticides, reduce environmental pollution and improve the safety of tobacco leaves and other plants.

Description

technical field [0001] The invention belongs to the technical field of plant pest control, and in particular relates to a method for regulating and controlling the emergence of aphids by hormones and temperature on the emergence of aphids from the aphid. Background technique [0002] Aphid [ Myzus persicae (Sulzer)] also known as peach aphid, belongs to Homoptera, Aphididae. The insect has a wide range of host plants, mainly Brassicaceae, Solanaceae, Rosaceae, Fabaceae, Chenopodiaceae and other plants. It uses its mouth needle to suck the juice of eating plants, which is very harmful. [0003] In the control of tobacco aphids, the extensive, large-scale and long-term use of chemical pesticides has caused the "3R (Residue, Resistance, Resurgence)" problem in tobacco leaf production to become more and more serious, making it more and more difficult to prevent and control aphids and aphid-borne viral diseases in tobacco leaf production . Aphids aphids ( Aphidius gifuensis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 顾钢赖荣泉白晶晶周挺舒静
Owner 中国烟草总公司福建省公司
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