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Phototoxicity detection method based on recombinant skin model

A technology of skin model and detection method, which is applied in the field of skin phototoxicity detection and research, can solve the problems of limited test conditions and phototoxicity detection, and achieve the effect of expanding the detection range

Inactive Publication Date: 2019-11-22
GUANGDONG BOXI BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phototoxicity in vitro replacement test is the general trend. At present, 3T3 fibroblast neutral red uptake test (3T3 NRU PT) has been approved by the European Union as an in vitro test method for skin phototoxicity test for chemical safety evaluation in EU countries, but As its test conditions are limited to water-soluble chemicals or cosmetic raw materials, this alternative test is limited in the phototoxicity testing of many cosmetic products

Method used

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  • Phototoxicity detection method based on recombinant skin model
  • Phototoxicity detection method based on recombinant skin model
  • Phototoxicity detection method based on recombinant skin model

Examples

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Effect test

Embodiment 1

[0045] The present embodiment provides a phototoxicity evaluation method based on the recombinant skin model Epikutis, comprising the following steps:

[0046] S1. Receiving and resuscitating the reconstructed skin model Epikutis;

[0047] S2. Select a light source;

[0048] S3. Determine the maximum tolerated dose of the recombinant skin model Epikutis;

[0049] S4. Detect the phototoxicity of the test substance.

[0050] Said step S1 comprises:

[0051] Culture the recombinant skin model Epikutis in a 6-well plate, 1 recombinant skin model Epikutis in each well, add 900 μL resuscitation solution per well, 36-38°C, 4-6% CO 2 Incubate in an incubator with 95% relative humidity for 55-65min, then replace the resuscitation solution, and incubate overnight for 18-22h. During the resuscitation process, discard the reconstructed skin model Epikutis that is damaged and has a large amount of water on the surface. The appearance of the reconstructed skin model Epikutis was evaluat...

Embodiment 2

[0067] The present embodiment provides a phototoxicity evaluation method based on the recombinant skin model Epikutis of a chemical chlorpromazine, comprising the following steps:

[0068] S1. Receiving and resuscitating the reconstructed skin model Epikutis;

[0069] S2. Select a light source;

[0070] S3. Determine the maximum tolerated dose of the recombinant skin model Epikutis;

[0071] S4. Detect the phototoxicity of the test substance.

[0072] Said step S1 comprises:

[0073] Culture the recombinant skin model Epikutis in a 6-well plate, 1 recombinant skin model Epikutis in each well, add 900 μL resuscitation solution per well, 36-38°C, 4-6% CO 2 Incubate in an incubator with 95% relative humidity for 55-65min, then replace the resuscitation solution, and incubate overnight for 18-22h. During the resuscitation process, discard the reconstructed skin model Epikutis that is damaged and has a large amount of water on the surface. The appearance of the reconstructed sk...

Embodiment 3

[0086] This example provides a phototoxicity evaluation method based on the recombinant skin model Epikutis for a finished cosmetic product F) [4], including the following steps:

[0087] S1. Receiving and resuscitating the reconstructed skin model Epikutis;

[0088] S2. Select a light source;

[0089] S3. Determine the maximum tolerated dose of the recombinant skin model Epikutis;

[0090] S4. Detect the phototoxicity of the test substance.

[0091] Said step S1 comprises:

[0092] Culture the recombinant skin model Epikutis in a 6-well plate, 1 recombinant skin model Epikutis in each well, add 900 μL resuscitation solution per well, 36-38°C, 4-6% CO 2 Incubate in an incubator with 95% relative humidity for 55-65min, then replace the resuscitation solution, and incubate overnight for 18-22h. During the resuscitation process, discard the reconstructed skin model Epikutis that is damaged and has a large amount of water on the surface. The appearance of the reconstructed ski...

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Abstract

The invention provides a phototoxicity detection method based on a recombinant skin model. The method comprises steps of S1, receiving and resuscitating the recombinant skin model; S2, selecting a light source; S3, determining the maximum tolerated dose of the recombinant skin model; and S4, testing the phototoxicity of a test object. The method is advantaged in that the method can detect not onlywater-soluble chemicals and cosmetic raw materials, but also can detect oily and creamy cosmetic finished products and different proportions of formulated substances, the method not only simulates the change in biological response of the human skin to different types of exogenous substances, but also expands the detection range of to-be-tested substances, is no longer limited to the water-solublechemicals, further includes finished cosmetics and formulas of various properties.

Description

technical field [0001] The invention belongs to the technical field of skin phototoxicity detection and research, and in particular relates to a phototoxicity detection method based on a recombinant skin model. Background technique [0002] Phototoxicity refers to a skin toxicity reaction caused by exposure to ultraviolet radiation after the skin is exposed to a chemical substance once, or a similar reaction that occurs after systemic application of a chemical substance and exposure to ultraviolet radiation, usually manifested as Erythema, blisters, hyperpigmentation and skin thickening etc. At present, the test methods used to evaluate the skin phototoxicity of cosmetics mainly include: animal test, human light patch test and in vitro test. [0003] Animal test, the current international animal test method is the animal skin light test, that is, to remove the hair on the back skin of the animal, and then apply a certain amount of the test substance to the depilated skin, a...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 王丹丹李潇张艳云胡成虎
Owner GUANGDONG BOXI BIO TECH CO LTD
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