Eurycoma longifolia jack extract and preparation method and application thereof

An extract, the technology of Tongkat, applied in the field of natural medicine, can solve the problems of decreased uric acid synthesis, kidney damage, heart-related death, etc., and achieve the effect of reducing uric acid level and promoting uric acid excretion

Inactive Publication Date: 2019-11-26
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Allopurinol can inhibit xanthine oxidase, so that hypoxanthine and xanthine cannot be converted into uric acid, that is, the synthesis of uric acid is reduced, thereby reducing the concentration of uric acid in the blood, and reducing the deposition of urate in bones, joints and kidneys. However, long-term Taking it can cause adverse reactions such as liver and kidney damage, gastrointestinal reactions, S-J syndrome, and severe hypersensitivity syndrome
On the other hand, febuxostat, as a xanthine oxidase inhibitor, is suitable for the long-term treatment of hyperuricemia with gout symptoms, however, it has been reported that febuxostat has a risk of heart-related death
[0006] In addition, uric acid excretion-promoting drugs mainly include benzbromarone, which can be used to treat patients with hyperuricemia with mild to moderate renal insufficiency. However, it has been withdrawn from the U.S. market because of its liver toxicity and damage to the kidneys.

Method used

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  • Eurycoma longifolia jack extract and preparation method and application thereof
  • Eurycoma longifolia jack extract and preparation method and application thereof
  • Eurycoma longifolia jack extract and preparation method and application thereof

Examples

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preparation example Construction

[0045] The preparation method of Tongkat Ali extract of the present invention comprises the following steps:

[0046] (i) extract Tongkat Ali root with an alcohol solution with a volume concentration of 50% to 90%, and concentrate the extract to obtain the extract;

[0047] (ii) extracting the extract obtained in step (i) to obtain an organic phase extract and an aqueous phase extract respectively;

[0048] (iii) The aqueous phase extract obtained in step (ii) is sequentially eluted with the following solvents on the macroporous adsorption resin column: (a) water or an alcoholic solution with a concentration lower than 40%, (b) 40 -75% alcohol solution, (c) 80-95% alcohol solution;

[0049] (iv) Concentrate the eluate obtained by eluting with (c) 80-95% alcohol solution in step (iii), and use it on a gel chromatography column in a volume ratio of 1:2 to 2:1 The alcohol / water solution was used for elution, and the eluate was collected, concentrated and dried to obtain the tot...

preparation example 1

[0072] Weigh 3.4 kg of Tongkat Ali root pieces, cut them into pieces, heat and reflux extract 3 times with 70% EtOH in 8 times the amount (extraction time is 3hr, 2hr, 2hr respectively), combine the three extracts, filter, and recover the solvent under reduced pressure to obtain Extract 160.0g.

[0073] Get above-mentioned extract 125.0g with 5.0L of EtOAc-H 2 O (1:1, v / v) extraction, obtain EtOAc layer extract 41.2g and H respectively 2 O layer extract 3.0L.

[0074] H 2 The O layer extract was loaded into the D101 macroporous adsorption resin column, followed by H 2 O and 95% EtOH were eluted to obtain H 2 O eluate 56.8 g, 95% EtOH eluate 31.3 g.

[0075] Load 25.0 g of the above 95% EtOH eluate onto a Sephadex LH-20 chromatographic column, and use MeOH-H 2 O (1:1, v / v) elution, every 50ml collects a fraction, collects 15 fractions altogether, merges each fraction, reclaims solvent under reduced pressure, obtains total diterpenoid component 12.1g (hereinafter referred to...

preparation example 2

[0077] Take 3 kg of Tongkat Ali root decoction pieces, cut into pieces, heat and reflux extract with 8 times the amount of 50% EtOH for 3 times (extraction time is 3hr, 2hr, 2hr respectively), combine the three extracts, filter, recover the solvent under reduced pressure, and obtain the infusion Cream 175.0g.

[0078] Get above-mentioned medicinal extract 120.0g and extract with the chloroform / water (1:1, v / v) of 5.0L, obtain chloroform layer extract 45g and H respectively. 2 O layer extract 3.0L.

[0079] H 2 The O layer extract was loaded into an AB-8 macroporous adsorption resin column, and was eluted with 20% EtOH, 50% EtOH, and 95% EtOH in turn to obtain 32.4 g of 20% EtOH eluate and 25.8 g of 50% EtOH eluate. g, 95% EtOH eluate 27.8g.

[0080] Load 20.0 g of the above 95% EtOH eluate onto a Sephadex LH-20 chromatographic column, and use MeOH-H 2 O (1:1, v / v) elution, every 50ml collects a fraction, collects 20 fractions altogether, merges each fraction, reclaims solv...

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Abstract

The invention provides an eurycoma longifolia jack (eurycoma longifolia jack) extract and a preparation method and application thereof. The eurycoma longifolia jack extract contains a eurycoma longifolia jack diterpene component of which the percentage by weight is 85%-95%. The eurycoma longifolia jack diterpene component disclosed by the invention has the effects of reducing the serum uric acid level of an experiment high-uric-acid animal model and promoting excretion of uric acid, and is hopeful to be further developed into a medicine for treating high uric acid.

Description

technical field [0001] The invention belongs to the field of natural medicines, and specifically relates to a Tongkat Ali extract, a preparation method thereof, and an application of the Tongkat Ali extract for treating hyperuricemia. Background technique [0002] In recent years, with the improvement of people's living standards, changes in lifestyle and diet structure, and population aging, the incidence of hyperuricemia in my country has increased year by year. According to statistics, the prevalence has reached 8.4% to 13.3%, and it has shown rejuvenation trend. [0003] Hyperuricemia is caused by an imbalance between uric acid production and excretion in the body. Uric acid in the body is mainly produced by purine metabolism, and the transformation process is mainly completed by xanthine oxidase; the excretion process of uric acid can be divided into several processes such as glomerular filtration and renal tubular reabsorption. About two-thirds of uric acid is excreted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P19/06
CPCA61K36/185A61K2236/333A61K2236/55A61P19/06
Inventor 王涛张祎温少诗包瑞霞仲一刘梦扬于海洋韩立峰
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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