Magnetic-gold dimer multifunctional nanoprobe and its preparation method and application
A technology of gold dimer and nanoprobe, which is applied in the fields of medical nanomaterials and nanobiomedicine, can solve the problems of large side effects, easy transfer and recurrence of tumor cells, and achieve high repeatability, high practical value, and operability strong effect
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[0031] Embodiments of the present invention also provide a method for preparing a magnetic-gold dimer multifunctional nanoprobe, such as figure 1 shown, including the following steps:
[0032] (1) Modification of gold nanoparticles:
[0033] Add the dispersion of gold nanoparticles into the PEG solution, stir the reaction, filter with an ultrafiltration membrane, and redisperse in the PBS buffer to obtain the PBS dispersion of Au-PEG;
[0034] Specifically, a dispersion of gold nanoparticles (with a size of 5-100nm in size and surface groups such as amino, carboxyl, or amido groups) at a concentration of 1-20mmol / L is added to a PEG solution with a concentration of 0.01-20mol / L (molecular weight 100 to 10,000), control the molar ratio of gold nanoparticles and PEG to 1:1 to 1:200, stir, and the reaction time is 0.1 to 2 hours; use ultrafiltration membrane filtration with a molecular weight cut off (MWCO) of 300 to 50,000, and the product Re-disperse in PBS buffer solution so...
Embodiment 1
[0044] (1) Add the dispersion of gold nanoparticles (the surface groups are amino, carboxyl, and amido) to the PEG solution with a concentration of 0.01-20mol / L, and control the molar ratio of gold nanoparticles and PEG to 1:1-1 :200, stirring, the reaction time is 0.1 ~ 2h; using ultrafiltration membrane filtration with a molecular weight cut off (MWCO) of 300 ~ 50000, the product is redispersed in PBS buffer solution, so that the concentration of Au-PEG is 1 ~ 100mmol / L.
[0045] (2) Fe 3 o 4 The nanoparticle (surface group hydroxyl, carboxyl, amino) dispersion is added to the PVP solution with a concentration of 0.1-200mol / L to control the Fe 3 o 4 The molar ratio of nanoparticles and PVP is 1:1~1:100, stirring, and the reaction time is 0.5~10h; use a centrifuge (speed 5000~10000rpm) to centrifuge for 0.2~1h; the product is dispersed in PBS buffer, so that Fe 3 o 4 - The concentration of PVP is 1-100 mmol / L.
[0046] (3) Add the PBS dispersion of Au-PEG obtained in ste...
Embodiment 2
[0048] (1) Add gold nanoparticles (surface group carboxyl) dispersion into the PEG solution with a concentration of 0.01mol / L, control the molar ratio of gold nanoparticles and PEG to 1:10, stir, and the reaction time is 1h; use The ultrafiltration membrane with a molecular weight cutoff (MWCO) of 4000 was filtered, and the product was redispersed in PBS buffer solution so that the concentration of Au-PEG was 10 mmol / L.
[0049] (2) Fe 3 o 4 The nanoparticle (surface group carboxyl group) dispersion is added to the PVP solution with a concentration of 0.1mol / L to control the Fe 3 o 4 The molar ratio of nanoparticles and PVP is 1:20, stirring, and the reaction time is 1h; use a centrifuge (rotating speed 8000rpm) to centrifuge for 0.5h; the product is dispersed in PBS buffer solution, so that Fe 3 o 4 - The concentration of PVP is 5 mmol / L.
[0050] (3) Add the PBS dispersion of Au-PEG obtained in step (1) into the NAC solution, control the molar ratio of Au-PEG to NAC to ...
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