A kind of Penicillium oxalicum for degrading organic acid and odor, its scale-up culture method and application

A Penicillium oxalicum, scale-up culture technology, applied in the field of Penicillium oxalicum and its scale-up culture, can solve the problem of limited deodorization effect of poultry manure

Active Publication Date: 2022-06-07
NANPING JIANYANG DISTRICT JIXIANG ANIMAL HUSBANDRY CO LTD
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Problems solved by technology

But the deodorizing effect of existing bacterial strains to poultry manure is still limited, therefore, the applicant has been committed to screening a microorganism with better deodorizing effect to poultry manure for a long time

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  • A kind of Penicillium oxalicum for degrading organic acid and odor, its scale-up culture method and application
  • A kind of Penicillium oxalicum for degrading organic acid and odor, its scale-up culture method and application
  • A kind of Penicillium oxalicum for degrading organic acid and odor, its scale-up culture method and application

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Embodiment Construction

[0021] The technical solutions of the present invention will be described in detail below with reference to the embodiments.

[0022] A kind of Penicillium oxalate degrading organic acid odor, which is Penicillium oxalic acid DH-1, the mold has been deposited in the China Center for Type Culture Collection on April 16, 2018, and the preservation number is CCTCC NO: M 2018201; preservation The address is: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0023] The Penicillium oxalate DH-1 of the present invention is separated and screened from the accumulated dairy cow breeding feces, and the specific steps are as follows:

[0024] (1) Take 0.2 g of dairy cow feces and inoculate it into 100 mL of liquid screening medium. The configuration steps of the liquid screening medium are: first prepare a basal medium, and the basal medium is based on glucose 20g / L, tryptone 20 g / L, yeast extract 10g / L, dry cow dung 5 g / L, adjust pH to 6.5~7.5, add water to volume, an...

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Abstract

The present invention provides a Penicillium oxalicum that degrades organic sour and odorous substances and its scale-up cultivation method and application. The Penicillium oxalicum is Penicillium oxalicum DH-1, which was preserved in China on April 16, 2018. Typical Culture Collection Center, the deposit number is CCTCC NO: M 2018201; the deposit address is: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The present invention collects samples from the piled dairy cow breeding feces, and inoculates the samples in the screening medium, in which various antibiotics, various organic acids, indole and skatole are added to induce and domesticate the bacteria in the samples , and separated and purified to obtain Penicillium oxalicum DH‑1, which has strong growth adaptability, high tolerance to organic acids and antibiotics, and can effectively degrade organic acids and odors, and is very suitable for dairy cow manure treatment.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganism application, and in particular relates to a Penicillium oxalate degrading organic acid odor and a method and application for its amplification. Background technique [0002] Fungi is an important part of the natural ecosystem, and its unique cell structure and biochemical and physiological functions endow it with many excellent characteristics. Compared with bacteria, fungi have great advantages in pollutant degradation, stress resistance, and environmental remediation. Among them, Penicillium has simple growth requirements and produces many secondary metabolites. It has been widely used in industrial enzyme production, biomedicine and other industries. It has strong adaptability and can use abundant and cheap raw materials or organic pollutants as Therefore, it has received more and more attention in the field of environmental pollution control in recent years. [0003] Peni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F11/02C02F103/20C12R1/80
CPCC12N1/14C02F11/02C02F2103/20C12R2001/80C12N1/145
Inventor 王宗寿施晞石神炳徐华锋潘建东许绍堂高宗耀魏发辉李振生谢忠卿
Owner NANPING JIANYANG DISTRICT JIXIANG ANIMAL HUSBANDRY CO LTD
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