A kind of asparagus clump bud induction method
A bud induction and asparagus technology, applied in the field of plant tissue culture, can solve the problems of low seed reproduction germination rate and long development cycle, and achieve the effects of increasing market supply, shortening growth cycle, and speeding up the process of rapid propagation.
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[0034] A method for inducing asparagus clumps buds, comprising the steps of:
[0035] (1) Pretreatment and disinfection of explant seeds: wash the surface of the seeds with washing powder, then rinse with running water for 2 hours, disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, rinse with sterile water for 3 times, and then use 0.1% Mercury chloride disinfection for 20 minutes, then rinsed with sterile water for 5 times, blotted the surface water with sterile absorbent paper, and connected into the germination medium; seeds germinated after 30 days; wherein explant seeds such as figure 1 shown.
[0036] (2) Seed germination and germination: the sterilized explant seeds were dried with sterile absorbent paper and then placed in a culture temperature controlled at (25±2) °C, the light intensity was 1500-2000lx, and the light time was 12h Cultured under the condition of / d; the seed-induced germination medium is MS medium without any hormones.
[0037] ...
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