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A method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles

A technology of magnetic nanoparticles and chlorella, which is applied in the field of transgenics, can solve problems such as limitations, and achieve the effects of high targeting, fast transfection speed, and convenient operation

Active Publication Date: 2021-05-28
BEIJING AGRO BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the continuous deepening of the research on Chlorella transgenic technology, there have been many reports on the successful transformation of Chlorella through gene gun method, Agrobacterium infection method, electric shock transformation method, PEG-mediated method, etc. The researchers successfully reported Genes or functional exogenous genes are transformed into Chlorella, and expression is achieved, but there are certain limitations in the above methods

Method used

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  • A method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles
  • A method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles
  • A method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles

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Embodiment Construction

[0037] 1 Materials and methods

[0038] 1.1 Experimental materials

[0039] 1.1.1 Algae and bacteria

[0040] Chlorella Ellipsoidea was purchased and purified from the freshwater algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences; the strain was Escherichia Coli DH5α strain.

[0041] 1.1.2 Reagents and instruments

[0042]Magnetic nanoparticle polyMAG200 (the magnetic nanoparticle polyMAG200 is ferric oxide magnetic nanoparticle with a diameter of 100nm), the magnetic plate was purchased from Chemicell Company, the reagents used for the preparation of the medium and solution were purchased from Sinopharm Group, and the antibiotics were purchased from Shanghai Baisai Biotechnology Co., Ltd., plasmid extraction kits were purchased from Tiangen Biochemical Technology Co., Ltd., cellulase, hemicellulose, and pectinase were purchased from Suleibao Technology Co., Ltd., and other chemical reagents were domestic analytical grade.

[0043] 1.1.3 Medium prepara...

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Abstract

The invention discloses a method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles, comprising the following steps: (1) constructing an antimicrobial peptide expression vector; (2) screening the sensitivity of chlorella to G418; (3) Magnetic nanoparticle-mediated transformation of chlorella, including pretreatment of chlorella cells as receptors, magnetic nanoparticle-gene construction and magnetic transformation of chlorella cells and screening of transformed chlorella, and obtaining transgenic chlorella after transformation . The method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles of the present invention has fast transfection speed, high efficiency and convenient operation.

Description

technical field [0001] The invention relates to the field of transgenes, in particular to a method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles. Background technique [0002] As a short peptide substance derived from natural organisms, antimicrobial peptides widely exist in animals, plants and microorganisms. More than a thousand kinds of antimicrobial peptides have been discovered so far. After continuous and in-depth research, it is found that a variety of antimicrobial peptides usually have the characteristics of short peptide chains and good thermal stability. Antimicrobial peptides have a different metabolism and mechanism of action from traditional antibiotics, exhibit broad-spectrum antibacterial properties against bacteria, fungi, viruses, etc., and hardly produce drug resistance. As the most potential active substance that is expected to replace antibiotics at present, and has characteristics that antibiotics do not have, the st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/62C12R1/89
CPCC07K14/43563C07K2319/02C07K2319/036C12N15/8206
Inventor 张秀海薛静曹苏珊陈绪清杨凤萍张铭芳杜运鹏高俊莲
Owner BEIJING AGRO BIOTECH RES CENT
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