PLIN gene SNP molecular marker, primer pair, kit related to meat quality of Yanbian yellow cattle and application of kit

A technology of molecular markers and Yanbian Yellow Cattle, which is applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of no reports of PLIN gene polymorphism traits, etc.

Active Publication Date: 2019-12-06
YANBIAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

It can be seen from the above that there are few studies on animals, especially on cattle, and t...

Method used

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  • PLIN gene SNP molecular marker, primer pair, kit related to meat quality of Yanbian yellow cattle and application of kit
  • PLIN gene SNP molecular marker, primer pair, kit related to meat quality of Yanbian yellow cattle and application of kit
  • PLIN gene SNP molecular marker, primer pair, kit related to meat quality of Yanbian yellow cattle and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Sample collection

[0056] The test cattle group selected 70 Yanbian yellow cattle bulls from Jilin Province Yanbian Animal Husbandry Development Group Co., Ltd., all the test cattle were fattened and slaughtered at the age of 30 months according to the unified standard feeding conditions, and slaughtered and divided according to the national unified standard. Add anticoagulant, aliquot and store at -80°C for later use.

[0057] 2. Extract DNA samples from Yanbian Yellow Cattle according to the instructions of the TIANGEN-Blood Genomic DNA Extraction Kit. The purity of the extracted Yanbian Yellow Cattle DNA sample was detected by ultraviolet spectrophotometer, and the OD 260 / OD 280 value. Agarose gel electrophoresis detection: use 1% agarose gel to detect the Yanbian Yellow Cattle DNA sample, and perform voltage electrophoresis for 30 minutes according to the fragment length; observe with a gel imaging system.

[0058] The results of the ultraviolet spectrophot...

Embodiment 2

[0080] 1. Experimental animals and sample collection

[0081] In this test cattle group, 70 Yanbian yellow cattle bulls from Jilin Province Yanbian Animal Husbandry Development Group Co., Ltd. were selected. All the test cattle were fattened and slaughtered at the age of 30 months according to the unified standard feeding conditions, and slaughtered and divided according to the national unified standard. , vacuum-packed and stored at low temperature for the determination of meat quality traits.

[0082] 2. Determination of main traits of cattle

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Abstract

The invention provides a PLIN gene SNP molecular marker, a primer pair and a kit related to meat quality of Yanbian yellow cattle and application of the kit, and belongs to the technical field of yellow cattle meat quality screening. The PLIN gene SNP molecular marker related to the meat quality of the Yanbian yellow cattle contains a nucleotide sequence with polymorphism of C/T located at 103bp of third exon of the PLIN gene. Correlation analysis is conducted on the polymorphism of the PLIN gene with the moisture content, the fat content, the protein content, the pre-slaughter weight, the carcass weight and the backfat thickness character of the Yanbian yellow cattle meat, the results show that 103bp C/T locus is significantly correlated with the fat content, the pre-slaughter weight, thecarcass weight and the backfat thickness of the Yanbian yellow cattle, and a situation is shown that the four characters of the fat content, the pre-slaughter weight, the carcass weight and the backfat thickness of the CT genotype are significantly higher than that of the CC genotype. The detection of the 103bp C/T locus of the Yanbian yellow cattle can be used for early selection of top-grade beef of the Yanbian yellow cattle.

Description

technical field [0001] The invention belongs to the technical field of yellow beef quality screening, and in particular relates to a Yanbian yellow beef quality-related PLIN gene SNP molecular marker, primer pair, kit and application thereof. Background technique [0002] With the continuous development of economy and agriculture, while people's quality of life is improved, people's diet structure is also gradually improved. Among the meats, beef has high nutritional value, and the proportion of beef consumption increases the fastest, so it is an ideal meat food. People's demand for high-grade beef is increasing day by day. Yanbian yellow cattle are mainly produced in Yanbian area of ​​Jilin Province in my country. They have the characteristics of Yanbian Korean nationality. They are one of the five local fine breed cattle and are high-quality local meat breeds in my country. Yanbian yellow cattle have adapted to the local natural environment for many years. They are stron...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 夏广军徐红艳徐畅张珈溯
Owner YANBIAN UNIV
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