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Radix astragali root source pyrenochaeta for efficiently inhibiting botrytis cinerea and application for radix astragali root source pyrenochaeta

A technology of Botrytis cinerea and Acanthospora, applied in the fields of application, fungicides, fungi, etc., can solve problems such as unseen strains

Active Publication Date: 2019-12-10
黑龙江省农业科学院植物保护研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the current research, there are no relevant reports on strains with good antagonistic effects on Botrytis cinerea

Method used

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  • Radix astragali root source pyrenochaeta for efficiently inhibiting botrytis cinerea and application for radix astragali root source pyrenochaeta
  • Radix astragali root source pyrenochaeta for efficiently inhibiting botrytis cinerea and application for radix astragali root source pyrenochaeta
  • Radix astragali root source pyrenochaeta for efficiently inhibiting botrytis cinerea and application for radix astragali root source pyrenochaeta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Screening and identification of Acanthospora SFJ12-R-5 from the root of Astragalus membranaceus that efficiently inhibits Botrytis cinerea

[0015] Plants to be tested: the wild plant Scutellaria baicalensis was collected from Xinsheng Village, Sancha Town, Arong Banner, Hulunbeier City, Inner Mongolia Autonomous Region.

[0016] Test medium: PDA medium (peeled potatoes 200g, glucose 20g, agar 18g, distilled water 1000mL.)

[0017] PD culture medium (peeled potatoes 200g, glucose 20g, distilled water 1000mL.)

[0018] method

[0019] 1. Isolation of Endophytic Fungi

[0020] Tissue separation method: the roots, stems and leaves of the plant samples were repeatedly washed with tap water and dried on filter paper. Take healthy tissues with roots 5-7mm long, stems 5-7mm long, and leaves 3mm×3mm square. Each tissue was soaked in 70% alcohol for 5 seconds, roots and stems were soaked in 0.1% mercury liter for 1.5-2 minutes, leaves were soaked in 0.1% mercury li...

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Abstract

The invention discloses radix astragali root source pyrenochaeta for efficiently inhibiting botrytis cinerea and application for the radix astragali root source pyrenochaeta and relates to the technical field of biology. A strain is named as pyrenochaeta nobilis in classification, is named as pyrenochaeta SFJ12-R-5, is deposited in General Microbial Center for China Committee for Culture Collection of Microorganisms (CGMCC) with a preservation number being CGMCC No.17766 and a preservation date being 31 May 2019, can be used for inhibiting the botrytis cinerea, and thus providing an environmental-friendly and efficient biological control method to prevention and treatment of the botrytis cinerea.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to an acanthospora from the roots of Astragalus membranaceus for inhibiting Botrytis cinerea and its application. Background technique [0002] Botrytis cinerea is a worldwide disease caused by Botrytis cinerea. It is one of the important diseases in tomato production in facilities. It generally causes yield loss of 10% to 20%, and can reach more than 60% in severe cases. . At present, due to the lack of effective disease-resistant varieties, chemical control is still one of the most effective means to control the disease. Because Botrytis cinerea has the characteristics of fast reproduction, large genetic variation, and strong adaptability, the pathogen has developed serious resistance to various chemical fungicides, which has become the main limiting factor for tomato protected cultivation (Li Baoju et al., 2003). At the same time, pesticide residues pollute the environment,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 杨帆李新民刘春来王爽刘亮蒋希峰刘宇徐充
Owner 黑龙江省农业科学院植物保护研究所
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