Method for measuring waterlogging resistance of Cornus Florida L.
A multi-flowered wood and waterlogging-resistant technology, which is applied in the agricultural field, can solve the problems of high measurement cost, complicated procedures, and highly toxic chemicals, and achieve the effects of reduced measurement costs, simple procedures, and high measurement efficiency
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Embodiment 1
[0055] 1 Materials and methods
[0056] 1.1 Materials
[0057] Prepare 60 biennial multiflora (Cornus florida L.) seedlings with vigorous plant growth and uniform height as the test material, each with 4-5 branches, 20 in a group, a total of 3 replicates, and moved to The flooding test was performed in a pot with a tray (22 cm in diameter and 19 cm in height). Before the first treatment, it is necessary to maintain the normal growth of ash tree. The test area is located in a ventilated and light-transmitting open area, and a canopy is set up to prevent rainwater from affecting the test.
[0058] 1.2 Processing method
[0059] From October 2, 2018, the flooding test was carried out. The bottom of the empty pot was covered with a plastic bag, and the potted seedlings were placed in the empty flowerpot. Water to ensure a flooding depth of 5cm. From each group, 5 pots were fixed for index determination, and the 3rd to 5th leaves under the growth point were selected for the de...
Embodiment 2
[0064] Determination of leaf color difference
[0065] Use a spectrophotometer (CM-700d / 600d) to measure the leaf color of the fixed leaves in the middle of the plant. The data measured for the first time is used as the standard color, and the subsequent data is the contrast color, and the color difference value of each test leaf is compared. The change of leaf color is studied by △L, △a, △b, △Eab and other indicators.
[0066] L, a, and b colors can perceive and measure any color, and △L, △a, △b, and △Eab can also be used to express the color difference between the standard sample and the test sample. △Eab represents the total color difference, which can be used as a The index is used to evaluate the color difference, the larger its value, the greater the color difference of the leaves, and vice versa, the smaller the color difference of the leaves.
[0067] figure 2 It shows that flooding has a great influence on △a, and the change fluctuates violently. The value of △a w...
Embodiment 3
[0069] spectrometry
[0070] The photosynthetic rate of leaves in the middle of the plant was measured with a spectrum analyzer (Unispec-SC), and fresh leaf samples were collected for the determination of various physiological indicators. After booting, press 1Preference on the page, adjust the Halogen source intensity to 100, create a new folder in DISK, select Enable auto saving, move the cursor and click OK, then clamp the whiteboard and press 2Data scan to confirm that the highest value of the curve is between 4.5 and 6.5 , press 8 to change Shutter open to Shutter closed, remove the whiteboard, press 4Dark scan for dark reaction, press 8 to change Shutter closed to Shutter open, press 5Reference Scan to clamp the whiteboard, clamp the leaf, and press the light hole to the surface of the leaf After 2Data scan, the data is measured.
[0071] Chlorophyll is the main pigment for photosynthesis in plants, so the strength of photosynthesis in plant leaves can be reflected by c...
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