Composition of DC vaccine and NKG2A antagonist and application of composition in anti-breast cancer or liver cancer

An anti-breast cancer and antagonist technology, applied in the field of anti-breast cancer or liver cancer, the composition field of DC vaccine and NKG2A antagonist, can solve the problem of weakening dendritic cell antigen presentation function and immune response can not play a significant role Therapeutic effect, tumor killing and other issues, to achieve the effect of improving the continuous effect of immune response, enhancing the body's immune effect ability, safety and effectiveness

Pending Publication Date: 2019-12-17
刘慧宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the cells in the tumor come from the human body itself, and some tumor cells also express molecules on the surface that prevent them from being recognized by the immune system (such as immune checkpoints), the immune response caused by most DC vaccines cannot successfully kill the tumor. Therapeutic effect is limited
[0012] 2. The target antigens of most DC vaccines are expressed not only on cancer cells but also on a few normal cells. The immune system has a certain probability of attacking normal cells
[0013] 3. The existing DC vaccines require DCs to present antigens to the immune system, and gradually initiate immunity. The effect is slow, and the treatment process is longer than other immunotherapies such as CAR-T.
[0014] 4. In addition, because many tumor ce

Method used

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  • Composition of DC vaccine and NKG2A antagonist and application of composition in anti-breast cancer or liver cancer
  • Composition of DC vaccine and NKG2A antagonist and application of composition in anti-breast cancer or liver cancer
  • Composition of DC vaccine and NKG2A antagonist and application of composition in anti-breast cancer or liver cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: DC vaccine and NKG2A antagonist composition treats breast cancer in mice

[0047] 1: Dendritic cells cultured from mouse bone marrow precursor cells

[0048] Extraction, culture and identification of myeloid-derived dendritic cells from Bal b / c mice

[0049] 1. Immediately immerse 6-7 week-old Balb / c mice in 75% ethanol after cervical dislocation. After 5 minutes, take out the mouse femur and tibia in a sterile environment and place them in RPMI-1640 for culture. Remove the muscle tissue on a sterile gauze pad, soak the clean bone in a new 70% ethanol plate for 2 minutes, and finally wash it twice with RPMI-1640 medium.

[0050] 2. Cut off the two ends of the bone (physis) with scissors and transfer it to another culture dish. Use a syringe to draw 2mL of RPMI-1640 medium to wash the bone marrow cavity to obtain bone marrow. Rinse with 1640 until the bone marrow cavity turns white. Cut up the epiphysis in another Petri dish. Mix the chopped epiphysis an...

Embodiment 2

[0086] Example 2: Composition of Ag-DC Vaccine and NKG2A Antagonist Combined Treatment of Liver Cancer Subcutaneously Transplanted Tumors in Mice

[0087] 1: Extraction, culture and identification of myeloid-derived dendritic cells from C57BL / 6J mice

[0088] 1. Immediately immerse 6-7-week-old C57BL / 6J mice in 75% ethanol after cervical dislocation. After 5 minutes, take out the mouse femur and tibia in a sterile environment and place them in RPMI-1640 for culture. Remove the muscle tissue on a sterile gauze pad, soak the clean bone in a new 70% ethanol plate for 2 minutes, and finally wash it twice with RPMI-1640 medium.

[0089] 2. Cut off the two ends of the bone (physis) with scissors and transfer it to another culture dish. Use a syringe to draw 2mL of RPMI-1640 medium to wash the bone marrow cavity to obtain bone marrow. Rinse with 1640 until the bone marrow cavity turns white. Cut up the epiphysis in another Petri dish. Mix the chopped epiphysis and bone marrow togethe...

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Abstract

The invention provides a composition of a DC vaccine and an NKG2A antagonist and application of the composition in anti-breast cancer or liver cancer. The DC vaccine in the composition is different from the early traditional tumor cell full-antigen-loaded DC vaccine, has specific targeting antigens, can more accurately treat tumors and reduce the killing side effects on normal cells; and secondly,the composition further contains a novel immune checkpoint inhibitor NKG2A antagonist which enables tumor cells with CD94-NKG2A/HLA-Ereceptor-ligand on the surface to be recognized and killed by NK cells and T cells, and an antigen presentation function of dendritic cells is enhanced, so that some cells which are not subjected to presentation and antigen by the DC vaccine are captured by an immune system again, the treatment effect on breast and liver cancer is enhanced, effect is faster than the conventional DC vaccine, the time of duration is longer, and the immunotherapy process is promoted.

Description

technical field [0001] The invention relates to the technical field of biological immunity, in particular to a composition of DC vaccine and NKG2A antagonist and its application in fighting breast cancer or liver cancer. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast, 99% of which occur in women. The number of new breast cancer cases worldwide ranks first among female malignant tumors every year. In recent years, the incidence of breast cancer in China has also increased year by year. In 2017, it was as high as 43 / 100,000. Currently, breast cancer is clinically divided into 4 types: Luminal A type (ER+ / PR+, HER-2-), Luminal B type (ER+ / PR+, HER-2+), HER-2+ type (ER- / PR- / HER-2+) and triple-negative breast cancer (ER- / PR- / HER-2-). Among them, the current status of triple-negative breast cancer accounts for 15%-23.8% of all breast cancer pathological types. There are about 400,000 to 700,000 patie...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/39A61K45/06A61P35/00
CPCA61K39/0011A61K39/39A61K45/06A61P35/00A61K2039/5154A61K2039/585A61K2039/55533A61K2039/55538A61K2039/55522A61K2039/812A61K2039/844A61K2300/00
Inventor 刘慧宁
Owner 刘慧宁
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