CRISPR/Sa-SauriCas9 gene editing system and application thereof

A gene editing and editing technology, applied in the field of gene editing, can solve the problems of complex PAM sequences, ineffective packaging, and difficulty in wide application, and achieve the effect of high editing activity
CN110577971AActive Publication Date: 2019-12-17FUDAN UNIV
4 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2019-12-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of gene editing, and in particular relates to a CRISPR / Sa-SauriCas9 gene editing system and application thereof. The gene editing system of the invention is a complex formed by Sa-SauriCas9 protein and sgRNA, and can accurately target DNA sequences and generate cleavage to cause double-strand breaks to DNA. The gene editing is to perform gene editing incells or in vitro; Sa-SauriCas9 is a fusion protein, and the PAM recognition domain (PAM interacting, PI) of SaCas9 is replaced with the PAM recognition domain (SauriCas9-PI) of SauriCas9. The Sa-SauriCas9 protein is small and is 1056 amino acids, and the recognized PAM sequence is simple. The Sa-SauriCas9 protein has the amino acid sequence as shown in SEQ ID NO: 1, and the sgRNA has the nucleotide sequence as shown in SEQ ID NO: 2. The invention has wide application prospects in the field of gene editing.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of gene editing, and in particular relates to a CRISPR / Sa-SauriCas9 system capable of gene editing in cells and related applications thereof. Background technique

[0002] CRISPR / Cas9 is an adaptive immune system evolved by bacteria and archaea to resist the invasion of foreign viruses or plasmids. In the CRISPR / Cas9 system, after crRNA (CRISPR-derived RNA), tracrRNA (trans-activating RNA) and Cas9 protein form a complex, they recognize the PAM (Protospacer Adjacent Motif) sequence of the target site, and the crRNA will form a complementarity with the target DNA sequence Structure, the Cas9 protein performs the function of cutting DNA, causing DNA breakage and damage. Wherein, tracrRNA and crRNA can be fused into a single-stranded guide RNA (singleguide RNA, sgRNA) through a linking sequence. When DNA is broken and damaged, two main DNA damage repair mechanisms in cells are responsible for repairing: non-h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More