Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Probe, gene chip and kit for detecting genes related to sudden exercise death

A gene chip and kit technology, applied in the field of biological detection, can solve the problem that genetic detection has not yet been studied, and achieve the effect of reducing detection time and cost, high accuracy and high signal resolution

Active Publication Date: 2020-11-10
ZHUHAI SINOCHIPS BIOSCIENCE CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although some studies have been done on gene detection of sudden cardiac death mutations, more studies on genes related to sudden death have not yet been carried out

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probe, gene chip and kit for detecting genes related to sudden exercise death
  • Probe, gene chip and kit for detecting genes related to sudden exercise death
  • Probe, gene chip and kit for detecting genes related to sudden exercise death

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] A probe for detecting genes related to sudden exercise death, the sequence of the probe is:

[0080] I736I probe: GTTCATTGATAGCAGGA;

[0081] I736T probe: GTTCACTGATAGCAGGA;

[0082] P731P probe: AGCGGCCATCCCTGAGGG;

[0083] P731S probe: AGCGGCCATCTCTGAGGG;

[0084] R723R probe: CCTCAGGTATCGCATCCT;

[0085] R723G probe: CCTCAGGTATGGCATCCT;

[0086] R663R probe: CAACTTGCGCTCCACCCA;

[0087] R663H probe: CAACTTGCACTCCACCCCA;

[0088] Y842Y probe: GTGTACGAGATGCGCGTC;

[0089] Y842X probe: GGTGTAGGAGATGCGCGT;

[0090] R190R probe: CTCTGGGGGCGGCTGCGC;

[0091] R190W probe: CTCTGGGGGTGGCTGCGC;

[0092] W305W probe: CGCTGTGGTGGGGGGTGG;

[0093] W305L probe: CGCTGTGGTTGGGGGTGG;

[0094] R2401R probe: CTCTTGGGACGCTGTGCTC;

[0095] R2401H probe: CTCTTGGGACACTGTGCTC.

[0096] A gene chip containing the above-mentioned probes for detecting genes related to sudden sports death, the preparation process of which comprises the following steps:

[0097] A silicon nitride f...

Embodiment 2

[0104] A kit for detecting folic acid gene polymorphism, comprising the gene chip in Example 1, PCR reaction system, hybridization buffer, washing solution, BW reaction solution and TMB chromogenic solution.

[0105] Wherein the composition of PCR reaction system is as shown in table 2:

[0106] Table 2: Composition of PCR reaction system

[0107]

[0108]

[0109] Wherein the sequence of the F-primer in the PCR reaction system is:

[0110] 663-F: 5'-TGCATCTCTTTCTGGCATT-3';

[0111] 723 / 731 / 736-F: 5'-TCTATGGGCAGAGCAGATCAC-3';

[0112] 842-F: 5'-TGCGGCTGAACTTCGACC-3';

[0113] 190-F: 5'-GAGTACGTGGTCCGCCT-3';

[0114] 305-F: 5'-CATCTTCTCCTCGTACTTTGTG-3';

[0115] 2401-F: 5'-GGAACGCGATGACC-3'.

[0116] Wherein the sequence of the R-primer in the PCR reaction system is:

[0117] 663-R: 5'-CCTCACCTGGAGACTTTG-3';

[0118] 723 / 731 / 736-R: 5'-ACTGGTTGTGATCAATGTCCAG-3';

[0119] 842-R: 5'-AGGCTCACCGATAGGCAT-3';

[0120] 190-R: 5'-TCCACAGGGCAGGCATGACTC-3';

[0121] 305-R...

Embodiment 3

[0128] The gene chips and kits prepared in Example 1-2 are used for detection, and the substance to be detected is a plasmid containing the SNP site of the target gene, and the type of the plasmid is as shown in Table 3:

[0129] Table 3: Plasmid types

[0130] plasmid gene of interest SNP site Plasmid 1 MYH7 R663R Plasmid 2 MYH7 R663H Plasmid 3 MYH7 R723R Plasmid 4 MYH7 R723G Plasmid 5 MYH7 P731P Plasmid 6 MYH7 P731S Plasmid 7 MYH7 I736I Plasmid 8 MYH7 I736T Plasmid 9 MYBPC3 Y842Y Plasmid 10 MYBPC3 Y842X Plasmid 11 KCNQ1 R190R Plasmid 12 KCNQ1 R190W Plasmid 13 KCNQ1 W305W Plasmid 14 KCNQ1 W305L Plasmid 15 R2 R2401R Plasmid 16 R2 R2401H

[0131] The above plasmids were synthesized and provided by Shanghai Sangon Bioengineering Co., Ltd.

[0132] Plasmids 1, 3, 5, 7, 9, 11, 13, and 15 were mixed to obtain mixed plasmids,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
diameteraaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of biological detection, and discloses probes, a gene chip and a kit for detecting genes related to athletic sudden death. The probes include an I736I probe, an I736T probe, a P731P probe, a P731S probe, an R723R probe, an R723G probe, an R663R probe, an R663H probe, a Y842Y probe, a Y842X probe, an R190R probe, an R190W probe, a W305W probe, a W305L probe, an R2401R probe and an R2401H probe. The gene chip contains the probes. The kit comprises the probes and a gene chip. Through selection of specific probes and primers, in combination with a genechip method, the kit has the advantages of good specificity, high accuracy, rapidness and convenience; higher signal resolution can be achieved through visible light optical amplification of the genechip; meanwhile, multi-site determination at a time of the genes related to athletic sudden death is realized, and the detection time and cost are reduced; and the detection result can be directly read by photographing or naked eyes, and is simple and convenient.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a probe, a gene chip and a kit for detecting genes related to exercise-related sudden death. Background technique [0002] Vigorous exercise or emotional agitation can induce syncope or sudden death. Among them, hypertrophic cardiomyopathy (HCM) and long QT interval (the total time course of ventricular depolarization and repolarization) syndrome (LQTS) are the main causes of exercise-related syncope or sudden death, and the above diseases are related to Genetic related. [0003] According to European and American studies, among young people or athletes under the age of 35, HCM is the most common cause of sudden death, accounting for about 1 / 500. At present, more than 900 gene mutations related to HCM have been discovered or identified, and they are distributed in 13 genes encoding cardiac sarcomere proteins. The main genes are MYH7 (encoding β-myosin h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6837C12N15/11
CPCC12Q1/6837C12Q1/6883C12Q2600/156
Inventor 王丽玲谢红光肖杰杭建宋家武
Owner ZHUHAI SINOCHIPS BIOSCIENCE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com