Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Device and method for cell breaking on piezoelectric substrate

A piezoelectric substrate and cell crushing technology, applied in the field of surface acoustic wave cell crushing devices and cell crushing devices, can solve the problems of complex manufacturing process, difficult integration and microfluidic system, and achieve no heating unit, solve Effects of cellular content damage

Inactive Publication Date: 2019-12-20
CHANGZHOU INST OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main advantage of this method is that it does not require chemical reagents, thereby avoiding the pollution of chemical reagents to the cell contents, but it requires a relatively complicated manufacturing process, and requires an external driving source such as a syringe pump to drive the cell fluid fluid, which is not easy to integrate with the microfluidic system. Room for improvement

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device and method for cell breaking on piezoelectric substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0018] The structure of the device for cell disruption on a piezoelectric substrate by surface acoustic waves is as follows: figure 1 As shown, it includes PCB board 1, piezoelectric substrate 2, pins on PCB board (including first pin 3, second pin 4, third pin 5, fourth pin 6), interdigitated The transducer 7 is a reflection grid 8, Teflon AF1600 hydrophobic thin layer 9 and a side wall with a thickness of 1-2 mm.

[0019] The polydimethylsiloxane spacer 10 has a height of 5-6 mm and is attached to the piezoelectric substrate. It is used to support the polydimethylsiloxane crossbar 11 on it, and the embedded width of the crossbar is 1 - 2 mm thin blade 12, the bottom of the thin blade is 0.2-0.3 mm away from the piezoelectric substrate, so as to avoid the thin blade from damaging the piezoelectric substrate and be as close as possible to the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a device and a method for cell breaking on a piezoelectric substrate. The device comprises a PCB; the PCB is connected with an external circuit through an electrical connectionend; the piezoelectric substrate is attached to the PCB; a hydrophobic thin layer is arranged on the piezoelectric substrate; side walls are arranged on the two sides of the hydrophobic thin layer; an interdigital transducer and a reflecting grating are arranged at the two ends of each side wall; a cross rod is erected above the hydrophobic thin layer; a blade close to the piezoelectric substrateis arranged on the cross rod; and a gap is reserved between the blade and the piezoelectric substrate. Without a high pressure, external driving of an injection pump, a heating unit and any chemicalreagent, the cell breaking on a micro-fluidic device can be realized, so that the problems of cell content damage and external driving source integration incapability in an existing method for cell breaking on the micro-fluidic device are solved.

Description

technical field [0001] The invention relates to a device for breaking cells in a microfluidic chip, in particular to a device and method for realizing cell breaking by surface acoustic waves, and belongs to the technical field of cell breaking. Background technique [0002] Cell disruption is a prerequisite for cell analysis, especially in microfluidic cell analysis, where on-chip cell disruption is required to achieve on-chip cell analysis, and a cell disruption unit must be integrated in the microfluidic analysis system. There are many macroscopic cell disruption methods, and the cell disruption technology is relatively mature. The common method is the mechanical crushing method, which uses high pressure to squeeze the cell fluid sample through a fixed small nozzle at high speed, and hits the impact target to break the sample cells. This method needs to generate high-pressure equipment, and needs to make a high-pressure chamber to accommodate the sample. At the same time,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/42C12M1/33C12N13/00
CPCC12M47/06C12N13/00
Inventor 董良威
Owner CHANGZHOU INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com