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Mycoplasma columbinum culture medium and application thereof

A technology of liquid culture medium and solid medium, which is applied in the field of Mycoplasma pigeonii culture medium, can solve the problems of low titer of viable bacteria, long culture time, slow growth, etc., and achieve high titer of viable bacteria, rapid separation and analysis.

Inactive Publication Date: 2019-12-20
黄明水
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Mycoplasma has high nutritional requirements on the culture medium, slow growth, and is difficult to separate. There are problems such as long cultivation time, low viable bacteria titer, and poor reproductive ability in the cultivation of Pigeon Mycoplasma in the prior art culture medium. Therefore, it is urgent to develop a suitable Medium for Rapid and Effective Culture of Mycoplasma pigeonii

Method used

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  • Mycoplasma columbinum culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Exploration of high-efficiency culture medium for Mycoplasma pigeonii

[0016] The sick pigeon came from a poultry farm in Fuzhou City, Fujian Province. At first, the sick pigeon had symptoms of runny nose, sneezing and coughing. Rapid death (74 in total). The dead pigeons were dissected and found that the nasal cavity and trachea were inflamed, the mucous membrane was thickened, and there was a large amount of viscous and purulent secretions, and the nasal tumor had grayish yellow deposits; Deposition of exudates; air sacculitis, rhinitis, pneumonia and pericarditis occurred; lesion tissues such as air sac exudate were taken, from which mycoplasma strain KM and Escherichia coli strain KE were isolated, and the diagnosis was mixed infection of mycoplasma and Escherichia coli.

[0017] The mixed infection of Mycoplasma and Escherichia coli in meat pigeons in this poultry farm compared with the previous simple Mycoplasma infection, the time from onset to death ...

Embodiment 2

[0023] The optimization, preparation and application of embodiment 2 Mycoplasma pigeonii high-efficiency medium

[0024] Further, optimize the FG liquid medium, the formula of the optimized FG1 liquid medium is: 75%-78% of FM-4 basic culture medium, 8%-12% of yeast extract (25%), and 2%-6% of pig serum , pigeon serum 0.4%-1.0%, polypeptide T1 (1ng / ml) 1.0%-1.5%, penicillin (1×10 5 IU / ml) 1%, phenol red (1%) 0.002%, pH adjusted to 7.6-7.8. Preferably, the optimal FG1 liquid medium formula is: 78% FM-4 basal culture medium, 10% yeast extract (25%), 4% pig serum, 0.5% pigeon serum, 1.2% polypeptide T1 (1ng / ml) , penicillin (1 x 10 5 IU / ml) 1%, phenol red (1%) 0.002%, pH adjusted to 7.6-7.8.

[0025] The formula of optimized FG3 liquid medium is: 75%-78% of FM-4 basic culture medium, 8%-12% of yeast extract (25%), 2%-6% of pig serum, 0.8-1.2% of pigeon serum, polypeptide T3 (1ng / ml) 1.5%-2.0%, penicillin (1×10 5 IU / ml) 1%, phenol red (1%) 0.002%, pH adjusted to 7.6-7.8. Pref...

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Abstract

The invention provides a novel efficient mycoplasma columbinum culture medium FG1. The culture medium is obtained through adding dove serum, and polypeptide T1 as shown in a SEQ ID: 1 sequence based on a conventional culture medium FM-4 of mycoplasma. The mycoplasma columbinum can realize rapid growth and proliferation in FG1 culture mediums, and can achieve very high viable bacteria titer, so that rapid detection and diagnosis of the mycoplasma columbinum are facilitated, rapid separation and resolution of the mycoplasma columbinum are facilitated, the development of mycoplasma columbinum vaccines and treatment medicines is facilitated, and research of disease mechanisms of the mycoplasma columbinum is facilitated.

Description

technical field [0001] The invention relates to the field of microbes, in particular to culture medium for mycoplasma pigeonii and application thereof. Background technique [0002] Mycoplasma is currently known as the prokaryotic microorganism with the smallest size and the simplest structure that can live independently. It has no cell wall, no flagella, no movement, Gram staining is negative, but it is not easy to color, and it shows polymorphic spheroids in Giemsa stained slides . Pigeon mycoplasma disease is a common fatal disease in pigeons. Its pathogenic bacteria is Mycoplasma pigeons. The main feature is that the sick pigeons have severe respiratory symptoms, manifested as cloudy air sacs, and there are caseous secretions in the nasal passages, trachea and bronchi. , Sick pigeons lose appetite, gradually become thin, and finally often die due to exhaustion or throat blockage by cheese-like substances, seriously affecting the health and life of carrier pigeons or mea...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/35
CPCC12N1/20
Inventor 黄明水陈永鑫
Owner 黄明水
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