Method for ameliorating bacteriolysis in early stage of hydroxyproline fermentation
A technology of hydroxyproline and the early stage of fermentation, applied in the field of fermentation engineering, can solve problems such as serious pollution, fermentation failure, economic loss, etc., and achieve the effects of fast growth rate, stable fermentation and increased yield
Active Publication Date: 2019-12-20
HENAN JULONG BIOLOGICAL ENG CO LTD
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Problems solved by technology
The first two methods have disadvantages such as high cost and serious pollution. With the development of metabolic engineering and the advantages of microbial production of amino acids in recent years, fermentation production of amino acids has become the most promising production method.
At present, the strain used for the expansion of L-hydroxyproline fermentation is Escherichia coli obtained by genetic recombination technology. During the test, the seeds grew normally, but after 70% of the batch cultured in the fermenter, a large number of In the case of bacterial lysis and OD drop, even if the culture continues to re-grow bacteria, the acid production is extremely low, resulting in fermentation failure and serious economic losses to production
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment
[0018] 1. Collect phage
[0019] Escherichia coli was used as the production bacteria, cultivated in the seed tank for 5 hours, the OD reached 9.20, there was no miscellaneous bacteria in the microscopic examination, and the transplantation index was reached, and the inoculum was transferred to a 30m3 fermenter according to 10% of the inoculum. Recovered, from 30% to 100%, microscopic examination found bacteriolysis, the fermentation liquid became clear, and the OD dropped from 12.0 to 2.0. Take the fermentation liquid at this time and inoculate it on the hydroxyproline bacteria-carrying plate for 8 hours for plate phage detection , refrigerated after the formation of phage plaques;
[0020] 2. Phage infection
[0021] Scrape a ring from the cultured hydroxyproline slant, transfer to 50mL shake flask liquid medium, culture for about 4 hours, OD reaches about 5.0, scrape a ring of phage from the prepared plaque plate The spots were transferred to the shake flask fermentation ...
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The invention relates to a method for ameliorating bacteriolysis in early stage of hydroxyproline fermentation, and belongs to the technical field of fermentation. The method comprises: firstly collecting contaminated phages during the process of hydroxyproline fermentation, infecting hydroxyproline fermentation broth with the collected phages, isolating and purifying the hydroxyproline strains that grow after infection, selecting seeds for upload tank verification, and screening excellent strains to conduct fermentation production. After the hyddroxyproline strain of the present invention isinfected with the phage, the seed vigor is obviously improved, and is not easily infected by the phage during the fermentation process, and the situation that hydroxyproline is prone to autolysis in the early stage of fermentation is effectively ameliorated.
Description
technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for improving bacteriolysis in the early stage of hydroxyproline fermentation. Background technique [0002] L-Hydroxyproline is one of the imino acids, widely used in pharmaceutical, chemical, food and beauty industries. There are three main production methods of L-hydroxyproline, which are biological tissue extraction method, chemical synthesis method and microbial fermentation method. The first two methods have disadvantages such as high cost and serious pollution. With the development of metabolic engineering and the advantages of microbial production of amino acids in recent years, fermentation production of amino acids has become the most promising production method. At present, the strain used for the expansion of L-hydroxyproline fermentation is Escherichia coli obtained by genetic recombination technology. During the test, the seeds...
Claims
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IPC IPC(8): C12Q1/10C12R1/19
CPCC12Q1/10
Inventor 张孟涛徐倩封浪张磊鹏刘永超马辉
Owner HENAN JULONG BIOLOGICAL ENG CO LTD