Culture method of wettability T cells

Abstract

The invention relates to a culture method of wettability T cells. The culture method comprises the following steps of (1) preparing and forming effector T cells; (2) using a differentiation culture medium, so that the effector T cells can be subjected to differentiation to TIL-TCM; and (3) amplifying TCM with an amplification culture medium. The cultured TIL-TCM has high tumor tropism, and can home to the position of tumors. Tumor cells can be specifically recognized, and tumors can be specifically killed. The culture method can be adaptive to microenvironment of tumors, can live in bodies fora long time, and can achieve the antitumor effect for a long term. A small quantity of the cells can achieve high antitumor effect, and the cells are short in culture time, low in probability of sideeffects, simple to operate and high in cell proliferation speed.

Description

technical field [0001] The invention relates to a method for cultivating infiltrating T cells, in particular to a method for cultivating tumor infiltrating central memory T cells. Background technique [0002] "2012 China Cancer Registration Annual Report" once reported that the incidence rate of cancer in my country is 285.91 / 100,000, and the urban and rural incidence rates are 303.91 / 100,000 and 249.98 / 100,000, respectively. It is estimated that by 2049, the incidence of cancer in my country may reach the level of 400 / 100,000. The main reason for the increase in the incidence of cancer is the aging of the population. Today, the proportion of my country's elderly population is 13.6%, and it may reach 16% in 20-30 years. Correspondingly, we can learn that the incidence of cancer has indeed been on a straight-line upward trend in recent years, and despite the continuous development of current cancer treatment measures, the physical condition of patients with advanced malign...

AI Technical Summary

Problems solved by technology

[0009] 1. TCM in peripheral blood "memorizes" different antigens and responds to different diseases and infections. Only a very small part of TCM can recognize tumors. Therefore, TCM isolated from peripheral blood has limited tumor killing ability, basically The expansions are all irrelevant cells;

[0010] 2. TIL is actually a collection of a group of immune cells, including various lymphocytes and even many tumor suppressor cells. With the development of science and technology, in recent years, it has been found that only a very small part of TIL has the ability to kill tumors , so how to expand tumor-specific TIL cells has always been the research focus of this cell. Existing methods such as neoantigen, monoclonal selection, etc. are very time-consuming and labor-intensive, and the cost is very high, which is difficult to promote;

[0011] 3. According to the National Institutes of Health (NIH), TIL needs 100 billion cells to reach an effective concentration and needs to be infused with high-dose IL2 to maintain the activity of TIL in the body. The reason is that traditional TIL is an effector T Cells, which are exhausted quickly in the body, cannot be expanded for a long time, so that the long-term anti-tumor effect cannot be achieved, and the expansion of 100 billion cells requires hundreds of liters of light culture medium, and the cost can be imagined;

[0012] 4. Large doses of IL2 will have great side effects on patients;

[0013] 5. Traditional peripheral blood amplified TCM, even if the tumor antigen is memorized, still cannot infiltrate into the tumor due to the tumor microenvironment and other reasons

[0014] To sum up, the advantages and disadvantages of TIL and TCM are complementary. If there is a kind of cell that can combine the advantages of the two and overcome the disadvantages of each other, then this kind of cell will have good therapeutic effect and huge market potential, but because TIL The content of TCM in TIL is extremely low, almost none, and it is impossible to directly separate TCM from TIL, and there is no complete solution at present

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