Unlock instant, AI-driven research and patent intelligence for your innovation.

Microscope system and method for image-guided micro-irradiation

A microscope system, microscope technology, applied in the field of microscope systems, can solve the problem of lack of high-throughput capability

Pending Publication Date: 2019-12-31
ACAD SINIC
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

STOMP (Spatial Targeted Optical Mini-Proteomics), proposed by Kevin CHadley et al. in 2015, is a manually operated technique using a commercially available two-photon system, but lacks the main components to achieve the high-throughput capabilities of the present invention

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microscope system and method for image-guided micro-irradiation
  • Microscope system and method for image-guided micro-irradiation
  • Microscope system and method for image-guided micro-irradiation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0092] Experimental Example 1: The system and method of this paper can be used to display the proteome of the nucleus, and its methodology is as follows Figure 5 shown. First, nuclei are imaged by staining with an anti-nuclear pore complex protein antibody. After the image is captured, image processing and analysis are performed to generate coordinate points that are expected to be scanned by the photosensitized light source. Then, control the galvanometer scanning vibrating mirror to scan these coordinate points. Exposure to light causes the photosensitizer to release free radicals and thus biotinylate the amino acids in the scanned area. Cameras and lights were performed over a large number of fields of view to generate sufficient biotinylated samples. Next, the biotinylated amino acids were purified and identified by mass spectrometry in the proteome of the nucleus. Proteomic analysis of biotinylated samples was performed using the systems and methods herein and, as sh...

experiment example 2

[0097] Experimental Example 2: The system and method herein can be used to identify proteins of stress granules in cells. First, stress granules are stained with a stress granule marker, such as G3BP1, and imaged. After the image is acquired, the image is processed and analyzed to generate a series of point coordinates that are expected to be scanned by the photosensitive light source. This paper enables the development of an image processing algorithm to precisely detect the location of stress granules, which facilitates further proteomic studies, such as Figure 4A and Figure 4B shown.

experiment example 3

[0098] Experimental Example 3: The system and method herein can be used to distinguish the difference of proteins in cancer cells with cancer stem cell markers (such as CD44) and those without cancer stem cell markers. In order to distinguish the difference between cancer cells and cancer stem cells, the protocol of this example is roughly the same as that of Experimental Example 1 above, except that stem cell markers are used for imaging in this experimental example.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a microscope system and method for image-guided micro-irradiation, and the microscope system comprises a microscope, an irradiation module, an image pick-up module, a first processing module and a second processing module, wherein the microscope comprises a stage for loading a sample; the image pick-up module may comprise a camera; the processing module is coupled to the microscope, the image pick-up module and the irradiation module; the processing module controls the image pick-up module so that the camera captures at least one image of the sample in the first field ofview; the image is transmitted to the processing module, and is processed automatically and in real time by the first processing module based on a preset condition to define a target area in the image, thereby obtaining coordinate information related to the target area; the coordinate information related to the target area is automatically transmitted to the processing module; and the processingmodule controls the irradiation module to irradiate the target area of the sample according to the received coordinate information related to the target area.

Description

technical field [0001] The present invention relates to a system and method for illuminating patterns on a sample, and more particularly to a microscope system and method for rapidly and sequentially illuminating different patterns over a large number of fields of view. Background technique [0002] There is currently a need to illuminate patterns at specific locations on a sample, such as a biological sample. Examples include molecular photobleaching at certain subcellular regions, photoactivation of fluorophores at defined locations, optogenetics, light-triggered release of reactive oxygen species within designated organelles, or light-induced protein labeling in defined structural features of cells Both require patterned irradiation. For some applications, the pattern of processing described above may need to be defined by microscopy images. Certain applications also require processing sufficient samples, adding high-throughput requirements to replicate processing in mu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G02B21/00G02B21/06G02B21/36
CPCG02B21/0048G02B21/06G02B21/361G02B21/365
Inventor 廖仲麒陈一德张至为钟颖文
Owner ACAD SINIC