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Application of bithionol for resisting biofilm infection of staphylococcus aureus

A technology of thiobisdichlorophene and staphylococcus, which is applied in the field of medicine, can solve the problems of poor treatment effect of biofilm infection, and achieve the effect of reducing repeated chronic infections

Pending Publication Date: 2020-01-03
SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many drugs against Staphylococcus aureus biofilm infection, only clindamycin, azithromycin, linezolid and daptomycin have been found to have certain therapeutic effects, but the treatment effect on some strains of biofilm infection is very poor

Method used

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  • Application of bithionol for resisting biofilm infection of staphylococcus aureus
  • Application of bithionol for resisting biofilm infection of staphylococcus aureus
  • Application of bithionol for resisting biofilm infection of staphylococcus aureus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Minimum inhibitory concentration (Minimum inhibitory concentration, MIC) detection:

[0028] The minimum inhibitory concentration (MIC) was carried out by the micro broth dilution method, with ATCC29213 as the quality control strain, and the operation was carried out according to the CLSI-M100-S27 version. The results are shown in Table 1 below. The MIC of thiobisdichlorophene to 25 clinically isolated Staphylococcus aureus strains (including methicillin-sensitive strain MSSA and methicillin-resistant strain MRSA) was 2.2 mg / L. Table 1. The minimum inhibitory concentration of thiobisdichlorophene to Staphylococcus aureus

[0029]

Embodiment 2

[0031] Thiobisdichlorophene can effectively remove the biofilm formed by Staphylococcus aureus test:

[0032] Staphylococcus aureus SA113 strain was cultured overnight in TSB medium at 37°C and 220rpm / min for 10-12h. Dilute the bacterial solution 1:200 with TSBG medium (TSB medium + 0.5% glucose), add 200ul per well to a 96-well plate (Costar3599), set 3 duplicate wells for each strain, and culture at 37°C for 24 hours to form a mature organism film. Discard the supernatant, wash with sterile normal saline 3 times (200ul / well / time), then add fresh TSBG medium (containing different concentrations of thiobisdichlorophene / thiochlorophene), and culture it statically for 48h (each After 24 hours, replace with a new medium), discard the supernatant, wash with PBS 3 times (200ul / well / time), dry at room temperature, add methanol to fix for 15min (200ul / well), discard methanol and dry at room temperature Add 100ul of 0.5% crystal violet staining solution to each well, stain at room t...

Embodiment 3

[0035] Sulfur bisdichlorophene / thiochlorophene can kill Staphylococcus aureus in biofilm test:

[0036] The Staphylococcus aureus strain was cultured overnight in TSB medium at 37°C and 220rpm / min for 10-12h. Dilute the bacterial solution 1:200 with TSBG medium (TSB medium + 0.5% glucose), add 2ml per well to a 24-well plate (Costar 3524), set 3 duplicate wells for each strain, and culture at 37°C for 24 hours to form a mature biofilm. Discard the supernatant, wash with sterile normal saline 3 times (200ul / well / time), then add fresh TSBG medium (containing 8x MIC concentration of thiobisdichlorophene or no drug), and culture statically for 48h ( Replace with new medium every 24 h). Discard the supernatant, wash with sterile normal saline for 3 times, then scrape off the biofilm at the bottom of the well with an autoclaved toothpick, resuspend in 1ml of normal saline, add trypsin to fully digest for 30 minutes, and then Wash twice with normal saline, then take 100ul after 1:...

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Abstract

The invention provides application of bithionol for resisting biofilm infection of staphylococcus aureus. The bithionol is used for resisting the biofilm infection of the staphylococcus aureus. The technical scheme of the application discloses new application of the bithionol, according to the application for resisting the biofilm infection of the staphylococcus aureus, the bithionol can effectively remove formed biofilms of the staphylococcus aureus in the environment wherein the biofilms of the staphylococcus aureus are infected, and can effectively kill the staphylococcus aureus in the biofilms. The bithionol at the subinhibitory concentration can further significantly inhibit formation the biofilms of the staphylococcus aureus.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an application of thiobisdichlorophene for resisting Staphylococcus aureus biofilm infection. Background technique [0002] Staphylococcus aureus can infect different parts of the human body and cause a variety of infectious diseases, ranging from common skin diseases such as folliculitis, acne, and stye to deep and fatal complications such as pneumonia, endocarditis, osteomyelitis, and other metastatic complications disease. Staphylococcus aureus can also adhere to the surface of human tissue cells or medical implant materials to form a biofilm structure composed of exopolysaccharide adhesion molecules, proteins, teichoic acid and extracellular DNA (eDNA), etc. Sensitivity to antibacterial drugs, and evasion of host immune cell attack and phagocytosis, resulting in chronic infection and protracted healing. The serious problem is that in recent years, with the wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/10A61P31/04
CPCA61K31/10A61P31/04
Inventor 郑金鑫余治健邓启文尚永朋林志伟陈重孙翔
Owner SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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