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Application of Baohuoside I to resisting of disinfection of staphylococcus aureus biofilms

A staphylococcal infection, staphylococcal technology, applied in the field of medicine, can solve the problem that Baohuogan I has no antibacterial properties, and achieve the effect of reducing repeated chronic infections

Pending Publication Date: 2020-10-02
华中科技大学协和深圳医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the antibacterial properties of Baohuogan I

Method used

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  • Application of Baohuoside I to resisting of disinfection of staphylococcus aureus biofilms
  • Application of Baohuoside I to resisting of disinfection of staphylococcus aureus biofilms
  • Application of Baohuoside I to resisting of disinfection of staphylococcus aureus biofilms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Experiment of Baohuogan I effectively inhibiting the formation of Staphylococcus aureus biofilm.

[0036] Select the SA113 standard strain positive for biofilm formation and a clinical strain YUSA139, and culture the strains overnight in TSB medium at 37°C and 220rpm / min for 10-12h. Dilute the bacterial solution 1:200 with TSBG medium (TSB medium+0.5% glucose) containing different concentrations of Baohuogan I, add 200ul per hole to a 96-well plate (Costar3599), set 3 duplicate holes for each strain, and 37 Cultivate at ℃ for 24 hours to form a mature biofilm, wash with PBS 3 times (200ul / well / time), dry at room temperature, add methanol for fixation for 15min (200ul / well), discard methanol and add 0.5% per well after drying at room temperature 100ul of crystal violet staining solution, dyeing at room temperature for 10min, gently eluting the crystal violet staining solution under clear water until the running water is colorless, read the OD on a microplate reader after...

Embodiment 2

[0039] Baohuogan I can effectively inhibit the biofilm formation of 108 clinical isolates of Staphylococcus aureus

[0040] 108 clinically isolated Staphylococcus aureus strains were selected, and the strains were cultured overnight in TSB medium at 37°C and 220rpm / min for 10-12h. Dilute the bacterial solution 1:200 with TSBG medium (TSB medium + 0.5% glucose) containing 25 μM Baohuogan I, add 200ul per well into a 96-well plate (Costar 3599), set 3 duplicate wells for each strain, and 37 Cultivate at ℃ for 24 hours to form a mature biofilm, wash with PBS 3 times (200ul / well / time), dry at room temperature, add methanol for fixation for 15min (200ul / well), discard methanol and add 0.5% per well after drying at room temperature 100ul of crystal violet staining solution, dyeing at room temperature for 10min, gently eluting the crystal violet staining solution under clear water until the running water is colorless, read the OD on a microplate reader after drying at room temperatur...

Embodiment 3

[0045] The combination of Baohuogan I and daptomycin can effectively remove the biofilm formed by Staphylococcus aureus.

[0046] Shake methicillin-sensitive Staphylococcus aureus (MSSA) SA113, YUSA86, CHS134 strains and methicillin-resistant Staphylococcus aureus (MRSA) YUSA139, YUSA142, YUSA145 strains overnight in TSB medium at 37°C and 220rpm / min Cultivate for 10-12h. Dilute the bacterial solution 1:200 with TSBG medium (TSB medium + 0.5% glucose), add 200ul per well to a 96-well plate (Costar 3599), set 3 duplicate wells for each strain, and culture at 37°C for 24 hours to form a mature biofilm. Discard the supernatant, wash with sterile normal saline 3 times (200ul / well / time), then add fresh TSBG medium (containing different concentrations of Baohuogan I and daptomycin combination), and culture it statically for 48h ( After changing the new medium every 24 hours), discard the supernatant, wash with PBS 3 times (200ul / well / time), dry at room temperature, add methanol to...

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Abstract

The invention discloses an application of Baohuoside I to resisting of disinfection of staphylococcus aureus biofilms. The Baohuoside I is used for resisting disinfection of the staphylococcus aureusbiofilms. The invention discloses a new application of the Baohuoside I, and an application of the Baohuoside I to resisting of disinfection of staphylococcus aureus biofilms. In the environment of disinfection of the staphylococcus aureus biofilms, the Baohuoside I can effectively restrain the formation of the staphylococcus aureus biofilms, and can be jointly combined with other antibiotics to effectively eliminate the formed staphylococcus aureus biofilms.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of Baohuogan I for resisting Staphylococcus aureus biofilm infection. Background technique [0002] Staphylococcus aureus can infect different parts of the human body and cause a variety of infectious diseases, ranging from common skin diseases such as folliculitis, acne, and stye to deep and fatal complications such as pneumonia, endocarditis, osteomyelitis, and other metastatic complications disease. Staphylococcus aureus can also adhere to the surface of human tissue cells or medical implant materials to form a biofilm structure composed of exopolysaccharide adhesion molecules, proteins, teichoic acid and extracellular DNA (eDNA), etc. Sensitivity to antibacterial drugs, and evasion of host immune cell attack and phagocytosis, resulting in chronic infection and protracted healing. The serious problem is that in recent years, with the wide application of ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/04A61L31/16A61L31/08A01N43/16A01P1/00
CPCA61K31/7048A61P31/04A61L31/16A61L31/08A01N43/16A61L2300/252A61L2300/404A61L2300/606
Inventor 尚永朋邓启文余治健
Owner 华中科技大学协和深圳医院
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