Method for regulating catalytic activity of protease in tanning process

A technology of catalytic activity and protease, applied in the field of regulating the catalytic activity of protease, leather processing and manufacturing, can solve problems such as leather damage, and achieve the effect of improving uniformity, reducing action time and strength

Active Publication Date: 2020-01-03
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is worth pointing out that the main enzyme component of the protease preparation for tanning is not collagenase, but other proteases; It is difficult to avoid the hydrolysis of skin collagen, resulting in the problem of leather wound and loose surface

Method used

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  • Method for regulating catalytic activity of protease in tanning process
  • Method for regulating catalytic activity of protease in tanning process
  • Method for regulating catalytic activity of protease in tanning process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The amounts of the following materials are all based on the mass percentage (wt%) of the raw hide.

[0031] Put the raw hide into the drum, add 400wt% water, adjust the temperature to 25°C, add 0.5wt% sodium carbonate, 0.5wt% degreasing agent and 1.0wt% protease preparation for water soaking (protease activity is 2000U / g) and 0.1wt% soybean trypsin inhibitor, after rotating for 30 minutes, adjust to intermittent rotation (10 minutes per hour), the total time is 24 hours, to obtain water-soaked skin.

Embodiment 2

[0033] The amounts of the following materials are all based on the mass percentage (wt%) of the raw hide.

[0034] Put the raw hide into the drum, add 200wt% water, adjust the temperature to 18°C, add 0.2wt% sodium carbonate, 0.2wt% degreasing agent and 0.1wt% protease preparation for water soaking (protease activity is 5000U / g) and 0.05wt% leupeptin, rotated for 30 minutes, then adjusted to intermittent rotation (10 minutes per hour), the total time is 12 hours, to obtain water-soaked skin.

Embodiment 3

[0036] The amounts of the following materials are all based on the mass percentage (wt%) of the raw hide.

[0037] Put the raw hide into the drum, add 300wt% water, adjust the temperature to 22°C, add 0.3wt% sodium carbonate, 0.3wt% degreasing agent and 0.5wt% protease preparation for water immersion (protease activity is 4000U / g) and 0.01wt% aprotinin, after rotating for 30 minutes, adjust to intermittent rotation (rotating 10 minutes per hour), the total time is 6 hours, to obtain water-soaked skin.

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Abstract

The invention discloses a method for regulating the catalytic activity of protease in a tanning process. A protease reversible inhibitor and an existing protease preparation are applied to a water soaking, depilating or softening process together to achieve the purpose of firstly inhibiting and then recovering the activity of the protease. The method can reduce the rate of the initial catalytic hydrolysis reaction of the protease in the tanning process, so after the protease permeates into skins, skin proteins are hydrolyzed, the action time and strength of the protease on collagen fibers on the skin surface layer are reduced, the uniformity of the hydrolysis effect of the protease on collagen fibers in every layer in the skins is improved, and the technical problems of easy damages to theskin collagen fibers and reduction of a leather product of a tanning biotechnology based on the effect of the protease are solve. The provided protease reversible inhibitor is selected from proteins,and is an environmentally-friendly assistant.

Description

technical field [0001] The invention belongs to the technical field of leather processing and manufacturing, and in particular relates to a method for regulating the catalytic activity of protease in the process of leather making and leather prepared by the method. Background technique [0002] Protease is more and more widely used in leather soaking, depilation, softening and other processes to catalyze and hydrolyze the removal of albumin, globulin, elastin, proteoglycan, keratin and other non-collagenous proteins in the hide, which can improve the soaking efficiency , Realize the purpose of cleaning and depilating and moderately dispersing fibers, and create suitable conditions for the penetration and combination of tanning and dyeing and finishing materials in the subsequent process. However, the substrate selectivity of the protease-catalyzed reaction is not high, and treating raw hide or bare hide with it will hydrolyze multiple proteins at the same time, including non...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C14C1/04C14C1/06C14C1/08
CPCC14C1/04C14C1/065C14C1/08
Inventor 曾运航王欢石碧王亚楠周建飞
Owner SICHUAN UNIV
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