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Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum samples and application method of device

A cerebrospinal fluid and hydrogen sulfide technology, which is applied in the preparation of test samples, material inspection products, biological testing, etc., can solve the problems of complicated equipment operation, high cost, cumbersome hydrogen sulfide pretreatment, etc., and achieve convenient operation and low cost. Effect

Active Publication Date: 2020-01-03
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at a series of problems such as cumbersome pretreatment, long time-consuming, complicated equipment operation and high cost in the detection of hydrogen sulfide in the complex environment of serum and cerebrospinal fluid, and proposes a device for rapidly extracting the content of hydrogen sulfide in cerebrospinal fluid and serum samples and its use method

Method used

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  • Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum samples and application method of device
  • Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum samples and application method of device
  • Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum samples and application method of device

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Embodiment

[0024] Such as figure 1 As shown, the device for quickly extracting hydrogen sulfide content in cerebrospinal fluid and serum samples of the present invention includes a centrifuge tube 1 containing sodium hydroxide solution, a 20 mL airtight syringe 2 containing phosphoric acid solution, and a micro-injection device for sucking the sample to be extracted Needle 3, the airtight syringe is composed of a syringe A, a rubber stopper A and a push rod A; the microsampling needle is composed of a syringe B, a rubber stopper B and a push rod B. .

[0025] In this embodiment, in order to make the experiment reasonable and effective, the water used in the experiment is ultrapure water; ultrapure water, also known as UP water, has a resistivity of 18 MΩ*cm (25°C).

[0026] In this example, the concentration of the phosphoric acid solution is 1 mol / L, and the volume is 5 mL; the concentration of the sodium hydroxide solution is 0.1 M, and the volume is 1 mL; the sample to be extracted i...

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Abstract

The invention relates to a device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum samples and an application method of the device. The device for rapidly extracting the hydrogen sulfide in the cerebrospinal fluid and serum samples comprises a 20 mL closed syringe containing a phosphoric acid solution, a centrifuge tube arranged at a syringe needle and a micro-syringe inserted at a syringe rubber plug, wherein a sodium hydroxide solution is contained in the centrifuge tube, and a sample to be extracted is sucked in the micro-syringe. The device and the method have the characteristics of being easy to operate, rapid to treat, low in cost and the like, and the hydrogen sulfide in the cerebrospinal fluid and serum samples can be rapidly extracted.

Description

technical field [0001] The invention relates to a device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum samples and its application method. Background technique [0002] Hydrogen sulfide (H 2 S) is a colorless, flammable, highly toxic gas with the stench of rotten eggs, mainly from factories, mining and petrochemical industries. If the wastewater containing hydrogen sulfide is untreated or discharged beyond the standard, it will cause serious damage to the ecological environment and even seriously threaten human health. Recent studies have confirmed that hydrogen sulfide is the third gas signaling molecule discovered after nitric oxide (NO) and carbon monoxide (CO), and has important roles in nerve conduction, inflammation, vasodilation, apoptosis, cell growth regulation, and cardiovascular protection. important role in physiological processes. The physiological level of hydrogen sulfide in mammalian serum is about 30-100 μM, while the level of h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N1/34
CPCG01N1/34G01N33/48
Inventor 林振宇陈超群罗芳邱彬郭隆华
Owner FUZHOU UNIV