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Seedling culture method using Platycerium wallichii Hook. spore sterile germination

A technology for germination of spores and fern spores, which is applied to the field of aseptic germination and development of staghorn fern spores, can solve the problems of long reproduction cycle, low reproduction efficiency, and low rooting rate of seedlings, and achieves reduction of culture cost, high reproduction efficiency, and seedling formation. short time effect

Active Publication Date: 2020-01-10
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above documents have reported the tissue culture technology of staghorn fern, it is only limited to the young true leaves as explants, and there are low reproductive efficiency (GGB induction rate 50%), long seedling propagation cycle (more than 8 months), rooting Low rate (less than 94%) and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Selection and disinfection of explants: Select the healthy mother plant of Staghorn fern difidoides as the object of explants, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into pieces with a size of 5.0×2.0cm. Put it into an ultrasonic cleaner filled with sterile water and shake it for 30 minutes, take it out and put it in a sterile container, put it on an ultra-clean workbench, blot up the water with a sterile paper towel, scrape off the spores with a sharp knife, and set aside.

[0037] Spore germination: use tweezers to pick up the spores and spread them evenly on the surface of the spore germination medium for cultivation; the spores are seeded and cultured for 10 days, and gradually turn from brown to green, and expand and grow, and 28 days after inoculation, they germinate into green spheroids (GGB) (the germination rate is as high as 100.0%), and then GGB proliferated in large quantities. After 30 days of inoculation,...

Embodiment 2

[0042] Selection and disinfection of explants: Select a healthy mother plant of staghorn fern as the object of explants, cut out the leaves with mature spores, rinse them with tap water first, then cut the leaves into pieces with a size of 5.0×2.0cm, put them in Vibrate and clean with an ultrasonic cleaner filled with sterile water for 35 minutes, take it out and put it in a sterile container, on the ultra-clean workbench, dry the water with a sterile paper towel, scrape off the spores with a sharp knife, and set aside.

[0043] Spore germination: use tweezers to pick up the spores and spread them evenly on the surface of the spore germination medium for cultivation; the spores are seeded and cultured for 12 days, and gradually turn from brown to green, and expand and grow, and germinate into green spheroids (GGB) after inoculation for 30 days (the germination rate is as high as 100.0%), and then GGB proliferated in large quantities. After 35 days of inoculation, the bottom of ...

Embodiment 3

[0048] Selection and disinfection of explants: Select the healthy mother plant of Staghorn fern difidoides as the object of explants, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into pieces with a size of 5.0×2.0cm. Put it into an ultrasonic cleaner filled with sterile water and shake it for 40 minutes, take it out and put it in a sterile container, put it on an ultra-clean workbench, blot up the water with a sterile paper towel, scrape off the spores with a sharp knife, and set aside.

[0049] Spore germination: use tweezers to pick up the spores and spread them evenly on the surface of the spore germination medium for cultivation; the spores are seeded and cultivated for 15 days, and gradually turn from brown to green, and expand and grow, and germinate into green spheroids (GGB) after inoculation for 35 days (the germination rate is as high as 100.0%), and then GGB proliferated in large quantities. After 40 days of inoculation,...

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Abstract

The invention provides a seedling culture method using Platycerium wallichii Hook. spore sterile germination. The method is characterized in that the leaves, with mature spores, of Platycerium wallichii Hook. are used as the explant raw materials, the formulas of culture media for various culture stages are designed, and rapid propagation is achieved through spore-GGB-bud. The method includes: selecting and sterilizing explants, performing spore germination, performing GGB differentiation culture, performing rooting culture and transplanting test-tube seedlings. The method has the advantages that by the method, the Platycerium wallichii Hook. seedlings can be obtained in only 178-210 days; the spore germination and GGB proliferation are performed synchronously, culture links are simplified, culture cost is lowered, high spore germination rate, high GGB proliferation rate and fast seedling acquiring, namely high propagation efficiency are achieved, and the efficiency of the whole seedling culture process is increased; in addition, the test-tube seedlings cultured by the method are robust and developed in root system, the seedlings are high in environment adaptability by the aid of acclimation, seedling transplanting survival rate is increased, and Platycerium wallichii Hook. rapid propagation is achieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for aseptic germination and seedling growth of staghorn fern spores. 【Background technique】 [0002] Staghorn fern (Platycerium wallichii Hook.) is a perennial evergreen herbaceous plant of the staghorn fern family Staghorn fern. It is native to Nabangba, Yingjiang County, southwest Yunnan, China, in the mountain rainforest at an altitude of 210-950 meters, Myanmar, northeastern India, Thailand and also have distribution. This species has been included in the national second-class protected plants. Its unique plant shape and graceful posture make it the most peculiar type of fern among the ornamental ferns. There are two types of leaves, which are divided into basal peltate leaves and spore leaves. The basal peltate leaves are also called sterile leaves or vegetative leaves, which are round, oval or fan-shaped, and are attached to the rhizome...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 叶秀仙陈艺荃方能炎林兵吴建设
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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