Seedling culture method using Platycerium wallichii Hook. spore sterile germination
A technology for germination of spores and fern spores, which is applied to the field of aseptic germination and development of staghorn fern spores, can solve the problems of long reproduction cycle, low reproduction efficiency, and low rooting rate of seedlings, and achieves reduction of culture cost, high reproduction efficiency, and seedling formation. short time effect
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[0035] Example one
[0036] Selection and disinfection of explants: Select the healthy mother plant of Staghorn fern as the explant material, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into 5.0×2.0cm pieces. Put it in an ultrasonic cleaner filled with sterile water and shake for 30 minutes, take it out and put it in a sterile container, place it on an ultra-clean workbench, and then use a sterile paper towel to absorb the water, scrape off the spores with a sharp knife, and set aside.
[0037] Spore germination: use tweezers to pick up the spores and evenly spread them on the surface of the spore germination medium for culture; spores are seeded and cultured for 10 days, starting from brown to green gradually, and swelling, inoculation 28 days to germinate into green globules (GGB) (germination rate is as high 100.0%), then GGB proliferates in large quantities, inoculated for 30 days, the bottom of the bottle is covered with dense GG...
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[0041] Example two
[0042] Selection and disinfection of explants: Choose the healthy mother plant of Staghorn fern as the explant material, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into 5.0×2.0cm pieces and put them in The ultrasonic cleaner filled with sterile water was shaken and cleaned for 35 minutes, taken out and placed in a sterile container, on the ultra-clean workbench, and then dried with a sterile paper towel, scraped off the spores with a sharp knife, and set aside.
[0043] Spore germination: Use tweezers to pick up the spores and evenly spread them on the surface of the spore germination medium for culture; spores are seeded and cultured for 12 days, starting from brown to green gradually, and swelling, inoculation 30 days to germinate into green globules (GGB) (germination rate is as high as 100.0%), then GGB proliferated in large quantities, inoculated for 35 days, the bottom of the bottle was covered with dense G...
Example Embodiment
[0047] Example three
[0048] Selection and disinfection of explants: Choose the healthy mother plant of Staghorn fern as the explant material, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into 5.0×2.0cm pieces. Put it in an ultrasonic cleaner filled with sterile water and shake it for 40 minutes. Take it out and put it in a sterile container. Place it on an ultra-clean workbench. Use a sterile paper towel to absorb the water. Use a sharp knife to scrape off the spores and set aside.
[0049] Spore germination: Use tweezers to pick up the spores and evenly spread them on the surface of the spore germination medium for culture; spores are seeded and cultured for 15 days, starting from brown to green gradually, and swelling, inoculating 35 days to germinate into green globules (GGB) (germination rate is as high as 100.0%), then GGB proliferated in large quantities, inoculated for 40 days, the bottom of the bottle was covered with dense G...
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