Seedling culture method using Platycerium wallichii Hook. spore sterile germination

A technology for germination of spores and fern spores, which is applied to the field of aseptic germination and development of staghorn fern spores, can solve the problems of long reproduction cycle, low reproduction efficiency, and low rooting rate of seedlings, and achieves reduction of culture cost, high reproduction efficiency, and seedling formation. short time effect

Active Publication Date: 2020-01-10
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above documents have reported the tissue culture technology of staghorn fern, it is only limited to the young true leaves as explants, and ther

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example one

[0036] Selection and disinfection of explants: Select the healthy mother plant of Staghorn fern as the explant material, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into 5.0×2.0cm pieces. Put it in an ultrasonic cleaner filled with sterile water and shake for 30 minutes, take it out and put it in a sterile container, place it on an ultra-clean workbench, and then use a sterile paper towel to absorb the water, scrape off the spores with a sharp knife, and set aside.

[0037] Spore germination: use tweezers to pick up the spores and evenly spread them on the surface of the spore germination medium for culture; spores are seeded and cultured for 10 days, starting from brown to green gradually, and swelling, inoculation 28 days to germinate into green globules (GGB) (germination rate is as high 100.0%), then GGB proliferates in large quantities, inoculated for 30 days, the bottom of the bottle is covered with dense GG...

Example Embodiment

[0041] Example two

[0042] Selection and disinfection of explants: Choose the healthy mother plant of Staghorn fern as the explant material, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into 5.0×2.0cm pieces and put them in The ultrasonic cleaner filled with sterile water was shaken and cleaned for 35 minutes, taken out and placed in a sterile container, on the ultra-clean workbench, and then dried with a sterile paper towel, scraped off the spores with a sharp knife, and set aside.

[0043] Spore germination: Use tweezers to pick up the spores and evenly spread them on the surface of the spore germination medium for culture; spores are seeded and cultured for 12 days, starting from brown to green gradually, and swelling, inoculation 30 days to germinate into green globules (GGB) (germination rate is as high as 100.0%), then GGB proliferated in large quantities, inoculated for 35 days, the bottom of the bottle was covered with dense G...

Example Embodiment

[0047] Example three

[0048] Selection and disinfection of explants: Choose the healthy mother plant of Staghorn fern as the explant material, cut the leaves with mature spores, rinse them with tap water first, and then cut the leaves into 5.0×2.0cm pieces. Put it in an ultrasonic cleaner filled with sterile water and shake it for 40 minutes. Take it out and put it in a sterile container. Place it on an ultra-clean workbench. Use a sterile paper towel to absorb the water. Use a sharp knife to scrape off the spores and set aside.

[0049] Spore germination: Use tweezers to pick up the spores and evenly spread them on the surface of the spore germination medium for culture; spores are seeded and cultured for 15 days, starting from brown to green gradually, and swelling, inoculating 35 days to germinate into green globules (GGB) (germination rate is as high as 100.0%), then GGB proliferated in large quantities, inoculated for 40 days, the bottom of the bottle was covered with dense G...

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Abstract

The invention provides a seedling culture method using Platycerium wallichii Hook. spore sterile germination. The method is characterized in that the leaves, with mature spores, of Platycerium wallichii Hook. are used as the explant raw materials, the formulas of culture media for various culture stages are designed, and rapid propagation is achieved through spore-GGB-bud. The method includes: selecting and sterilizing explants, performing spore germination, performing GGB differentiation culture, performing rooting culture and transplanting test-tube seedlings. The method has the advantages that by the method, the Platycerium wallichii Hook. seedlings can be obtained in only 178-210 days; the spore germination and GGB proliferation are performed synchronously, culture links are simplified, culture cost is lowered, high spore germination rate, high GGB proliferation rate and fast seedling acquiring, namely high propagation efficiency are achieved, and the efficiency of the whole seedling culture process is increased; in addition, the test-tube seedlings cultured by the method are robust and developed in root system, the seedlings are high in environment adaptability by the aid of acclimation, seedling transplanting survival rate is increased, and Platycerium wallichii Hook. rapid propagation is achieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for aseptic germination and seedling growth of staghorn fern spores. 【Background technique】 [0002] Staghorn fern (Platycerium wallichii Hook.) is a perennial evergreen herbaceous plant of the staghorn fern family Staghorn fern. It is native to Nabangba, Yingjiang County, southwest Yunnan, China, in the mountain rainforest at an altitude of 210-950 meters, Myanmar, northeastern India, Thailand and also have distribution. This species has been included in the national second-class protected plants. Its unique plant shape and graceful posture make it the most peculiar type of fern among the ornamental ferns. There are two types of leaves, which are divided into basal peltate leaves and spore leaves. The basal peltate leaves are also called sterile leaves or vegetative leaves, which are round, oval or fan-shaped, and are attached to the rhizome...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 叶秀仙陈艺荃方能炎林兵吴建设
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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