Composition containing dehydromanchophylline and skin care application thereof

A technology of dehydrorhynchophylline and its composition, which is applied in the field of traditional Chinese medicine and cosmetics, can solve the problems of unstable properties of ascorbic acid derivatives, excessive synthesis of melanin, poisoning, etc., and achieve the effect of improving the effect, enhancing the sun protection effect, and fair skin

Active Publication Date: 2021-12-07
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, kojic acid and arbutin are easy to oxidize and change color. Kojic acid will oxidize at high concentrations and have a negative impact on the thyroid gland; the effect of arbutin on the content of melanin is bidirectional, and improper use may lead to excessive synthesis of melanin ; Hydroquinone, as a mercury compound, is irritating to the skin to a certain extent, and may cause poisoning in severe cases, so it has been restricted or banned from being added to cosmetics in some countries; ascorbic acid derivatives have unstable properties, and It is only effective at high concentrations, and is easily restricted by the formula in specific use

Method used

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  • Composition containing dehydromanchophylline and skin care application thereof
  • Composition containing dehydromanchophylline and skin care application thereof
  • Composition containing dehydromanchophylline and skin care application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Effects of dehydrorhynchophylline, echinacoside, and andrographolide on cell viability.

[0045] Mouse melanoma cells B16F10 were treated with 1×10 4 100 μL of melanocyte culture medium was added to a 96-well plate. After 24 hours, the cells adhered to the wall, discarded the original medium, and added different concentrations of dehydrorhynchophylline (0 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL) or Andrographis paniculata Esters (0 μg / mL, 10 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL) in fresh medium. After 48h, add the medium containing WTS and place in CO 2 Incubate in an incubator for about 45 minutes, and measure its absorbance at 490 nm. The experiment was repeated three times. The result is as Picture 1-1 , 1-3, compared with the control group without drugs, when the concentration of dehydrorhynchophylline is lower than or equal to 100 μg / mL, and the concentration of andrographolide is lower than or equal to 50 μg / mL will n...

Embodiment 2

[0047] Example 2. The inhibitory effect of dehydrorhynchophylline on the melanin content of B16F10 cells.

[0048] In order to evaluate the whitening efficacy of dehydrorhynchophylline alone, the following melanin production inhibition test was carried out at the cellular level.

[0049] B16F10 cells at 1×10 per well 4 The cell density of each was inoculated in a 96-well plate. After 24 hours, the original medium was discarded, and kojic acid containing 50 μg / mL (as a positive control), and 25 μg / mL, 50 μg / mL, 75 μg / mL, and 100 μg were added respectively. / mL dehydrorhynchophylline-free phenol red medium, after 72h, take the supernatant of all well plates, and detect their absorbance at 490nm with a microplate reader. The experiment was repeated three times. The result is as figure 2 As shown, compared with the control group, the melanin content secreted by the cells of the kojic acid group and the different concentrations of dehydrorhynchophylline groups were significantly...

Embodiment 3

[0050] Example 3. Comparison of the inhibitory effects of the composition and dehydrorhynchophylline, echinacoside and andrographolide on the melanin content of B16F10 cells.

[0051] In order to evaluate the whitening efficacy of the composition after use with dehydrorhynchophylline, echinacoside and andrographolide, the following melanin production inhibition test was carried out at the cellular level.

[0052] B16F10 cells at 1×10 per well 4 The cell density of each was inoculated on a 96-well plate, and the original medium was discarded after 24 hours, and 50 μg / mL kojic acid (as a positive control group), a composition (containing 25 μg / mL dehydrorhynchophylline and 25 μg / mL kojic acid) were added respectively. phenol red-free medium composed of echinacoside and 5 μg / mL andrographolide), 50 μg / mL dehydrorhynchophylline, 50 μg / mL echinacoside and 10 μg / mL andrographolide, and all well plates were taken after 72 hours The supernatant was detected by a microplate reader for...

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Abstract

The invention discloses the application of dehydrorhynchophylline, a natural plant monomer, and the composition of the monomer, echinacoside and andrographolide in preparing whitening cosmetics, and belongs to the field of traditional Chinese medicine and cosmetics. Dehydrorhynchophylline or its composition with andrographolide and echinacoside as whitening active ingredients can jointly inhibit the generation of melanin in skin cells and reduce the damage of ultraviolet rays (UV) to skin cells, and also has the effect of improving skin fibrogenesis. The ability of cells to synthesize collagen has the effect of anti-photoaging. The combination of the three monomers dehydrorhynchophylline, andrographolide, and echinacoside has obvious synergistic effect, the efficacy is significantly improved, and it is safe and stable. It can lighten pigmentation, whiten skin, improve human skin elasticity and moisture, and has Anti-photoaging effect. The plant monomer with whitening effect of the present invention can be used as an effective active ingredient to make cream, lotion, essence, facial mask, spray and other dosage forms for use in cosmetics.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and cosmetics, and relates to a natural plant monomer composition with whitening function, which can be applied to skin care, especially cosmetics for skin whitening, lightening spots, sun protection and anti-photoaging. Background technique [0002] Orientals have regarded white as beauty since ancient times, and white skin is regarded as a symbol of nobility and dignity. Under the traditional concept of one white covering all ugliness, the fashion of white skin continues to this day. Today's environmental pollution is intensifying, and human skin is exposed to more ultraviolet rays. Modern urban life is fast-paced and irregular, which can easily lead to disordered biological clocks, easy deposition of skin pigment, and dull complexion. Economic development has also prompted consumers to have higher requirements for the inherent quality of products, and product safety and effectiveness...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/74A61K31/438A61P17/00A61P17/16A61P17/18A61K8/9789A61K8/49A61K8/60A61Q17/04A61Q19/00A61Q19/02A61Q19/08A61K36/19A61K31/365A61K31/7048A61K36/64
CPCA61K8/492A61K8/4973A61K8/602A61K31/365A61K31/438A61K31/7048A61K36/19A61K36/64A61K36/74A61Q17/04A61Q19/00A61Q19/004A61Q19/02A61Q19/08A61K8/9789A61P17/00A61P17/16A61P17/18A61K2300/00
Inventor 叶希韵周艳卿尹尉翰刘明耀
Owner EAST CHINA NORMAL UNIV
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