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Cell extracting solution storage device for cell-free protein synthesis

A cell-free protein and storage device technology is applied in the field of cell extract storage devices for cell-free protein synthesis. , to avoid the effect of degeneration

Inactive Publication Date: 2020-01-10
JIANGSU ZHIDIAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In cell-free protein synthesis, it is necessary to extract some cell fluid for experimental observation. The cell extract needs to be stored under specific temperature conditions. The existing storage device has a single function and cannot effectively protect the extract. It is not convenient to take out the test tube

Method used

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  • Cell extracting solution storage device for cell-free protein synthesis
  • Cell extracting solution storage device for cell-free protein synthesis
  • Cell extracting solution storage device for cell-free protein synthesis

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Embodiment Construction

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0021] refer to Figure 1-4 , a storage device for cell extract solution for cell-free protein synthesis, comprising a storage box 1, a rectangular opening is opened on one side of the outer wall of the storage box 1, and a hermetic door 2 is hinged on one side of the inner wall of the rectangular opening, and the outer walls of both sides of the storage box 1 are Vents are provided, and the inner walls of the two vents are provided with filter covers 3, the two ends of the inner walls on both sides of the storage box 1 are fixed with slide rails 5 by bolts, and the inner walls on both sides of the four slide rails 5 slide respectively Two mesh plates 6 are connected...

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Abstract

The invention discloses a cell extracting solution storage device for cell-free protein synthesis. The cell extracting solution storage device for cell-free protein synthesis includes a storage box, the outer wall of one side of the storage box is provided with a rectangular opening, the inner wall of one side of the rectangular opening is provided with a sealing door in a hinged mode, the outer walls of the two sides of the storage box are provided with ventilation openings correspondingly, the inner walls of the two ventilation openings are provided with filter covers correspondingly, sliding rails are fixed to the two ends of the inner walls of the two sides of the storage box correspondingly through bolts, the inner walls of the two sides of the four sliding rails are slidably connected with two mesh plates correspondingly, a temperature sensor is fixed to the inner wall of the top of the storage box through bolts, and the same partition plate is fixed to the inner walls of the twosides of the storage box through bolts. According to the cell extracting solution storage device, by arranging the two mesh plates on the four sliding rails to facilitate pull of the mesh plates, thus pick and placement of time are facilitated, the interior of the storage box is cooled through cooperation between a compressor, a condensing tube and a cooling box, denaturation of an extracting solution due to temperature increase is avoided, and on and off of an installation tube are conveniently controlled through arrangement of a thermal baffle.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a cell extract storage device for cell-free protein synthesis. Background technique [0002] The cell-free protein synthesis system (The cell-free protein synthesis system) is an in vitro system that uses exogenous mRNA or DNA as a template and supplements substrates and energy in the enzyme system of cell extracts to synthesize proteins. Compared with the traditional in vivo recombinant expression system, the in vitro cell-free synthesis system has many advantages, such as the ability to express special proteins that are toxic to cells or contain unnatural amino acids (such as D-amino acids), and can directly use PCR products as templates. Simultaneously synthesize multiple proteins in parallel to carry out high-throughput drug screening and proteomics research. [0003] In cell-free protein synthesis, it is necessary to extract some cell fluid for experimental observatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/34C12M1/04
CPCC12M29/04C12M29/06C12M41/24
Inventor 连佳长
Owner JIANGSU ZHIDIAN BIOTECHNOLOGY CO LTD