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Method for improving yield of enzymatic hydrolysis of cellulose

A cellulase and enzymatic hydrolysis technology, which is applied in the field of improving cellulose enzymatic hydrolysis yield by acid salt pretreatment, can solve the problems of high operating cost, low enzymatic hydrolysis yield, and high equipment requirements, so as to reduce corrosion and increase yield efficiency, reduce equipment investment and maintenance costs

Pending Publication Date: 2020-01-10
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems of low enzymatic hydrolysis yield, high equipment requirements and high operating costs in the existing lignocellulosic raw material pretreatment technology, and provides a method for improving the enzymatic hydrolysis yield of cellulose by using acidic salt pretreatment, and specifically provides a A method for efficient enzymatic hydrolysis and saccharification of cellulose and obtaining high-concentration reducing sugars

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Adopt poplar as raw material, accurately weigh 50.0g absolute dry material in steel tank reactor, add concentration according to the solid-liquid ratio of 1:6 and be 5% magnesium acetate solution (according to absolute dry raw material), adjust solution pH with acetic acid The value is 4.5. Stir evenly and seal it. Put it into a cooker and heat it to 160°C and keep it warm for 20 minutes. Take out the reaction kettle immediately and cool it in flowing cold water to terminate the reaction. The material residue was washed 3 times with deionized water until the washing solution was neutral.

[0030] After the fiber residue is air-dried, grind to 40-60 mesh, weigh 2.0g powder and place it in a 250mL conical flask, add 30FPU / g cellulase and 20CBU / g cellobiase, add a drop of ethyl acetate for To prevent the generation of miscellaneous bacteria and microorganisms during the hydrolysis process, 100 mL of 0.1 mol / L citric acid-sodium citrate buffer solution (pH=4.8) was added, a...

Embodiment 2

[0032] Adopt poplar as raw material, accurately weigh 50.0g absolute dry material in steel tank reactor, add concentration according to the solid-liquid ratio of 1:6 and be 5% magnesium acetate solution (according to absolute dry raw material), adjust solution pH with acetic acid The value is 4.5. Stir evenly and then seal it. Put it into a cooker and heat it to 170°C and keep it warm for 20 minutes. Take out the reaction kettle immediately and cool it in flowing cold water to terminate the reaction. The material residue was washed 3 times with deionized water until the washing solution was neutral.

[0033]After the fiber residue is air-dried, grind to 40-60 mesh, weigh 2.0g powder and place it in a 250mL conical flask, add 30FPU / g cellulase and 20CBU / g cellobiase, add a drop of ethyl acetate for To prevent the generation of miscellaneous bacteria and microorganisms during the hydrolysis process, 100 mL of 0.1 mol / L citric acid-sodium citrate buffer solution (pH=4.8) was adde...

Embodiment 3

[0035] Adopt poplar as raw material, accurately weigh 50.0g absolute dry material in steel tank reactor, add concentration according to the solid-liquid ratio of 1:6 and be 5% magnesium acetate solution (according to absolute dry raw material), adjust solution pH with acetic acid The value is 4.5. Stir evenly and seal it. Put it into a cooker and heat it to 180°C and keep it warm for 20 minutes. Take out the reaction kettle immediately and cool it in flowing cold water to terminate the reaction. The material residue was washed 3 times with deionized water until the washing solution was neutral.

[0036] After the fiber residue is air-dried, grind to 40-60 mesh, weigh 2.0g powder and place it in a 250mL conical flask, add 30FPU / g cellulase and 20CBU / g cellobiase, add a drop of ethyl acetate for To prevent the generation of miscellaneous bacteria and microorganisms during the hydrolysis process, 100 mL of 0.1 mol / L citric acid-sodium citrate buffer solution (pH=4.8) was added, a...

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Abstract

The invention discloses a method for improving the yield of enzymatic hydrolysis of cellulose, and belongs to the field of enzymatic hydrolysis methods of fiber raw materials. The method for improvingthe yield of enzymatic hydrolysis of the cellulose comprises the steps that an acidic salt solution is used for pretreating the fiber raw materials, and the acidic salt solution is an alkali metal salt solution or an alkaline earth metal salt solution of small molecule organic acid. Compared with the prior art, high-efficiency enzymatic saccharification of the cellulose can be achieved, high-concentration reducing sugar can be obtained, and good application value is achieved through the method for improving the yield of enzymatic hydrolysis of the cellulose.

Description

technical field [0001] The invention relates to the field of enzymatic hydrolysis methods for fiber raw materials, and specifically provides a method for improving the yield of cellulose enzymatic hydrolysis by using acidic salt pretreatment. Background technique [0002] At present, with the intensified consumption of fossil energy such as oil and coal in the world, the problems of energy shortage and environmental pollution are becoming more and more serious, and the environment-friendly and sustainable biomass energy has attracted widespread attention. The second-generation bioethanol, which uses lignocellulose as raw material, is the most promising type of biomass energy. It has the advantages of wide source of raw materials, low cost and no competition with human beings for food. At present, it has realized industrial production. However, lignocellulosic raw materials have a compact structure and have strong "resistance" to enzymes and microorganisms, so it is difficult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/14C12P19/02C12P2201/00C12P2203/00
Inventor 陈晓倩杨秋林李宗全傅英娟秦梦华
Owner QILU UNIV OF TECH
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