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Method for improving enzymolysis saccharifing yield of lignocellulose

A lignocellulosic enzyme and lignocellulose technology, applied in biofuels, fermentation, etc., can solve the problems of low fermentable sugar concentration, low enzymatic hydrolysis efficiency, high production cost, etc., achieve abundant sources, and increase enzymatic hydrolysis and saccharification yield , the effect of price regeneration

Active Publication Date: 2015-11-11
SOUTH CHINA UNIV OF TECH
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  • Abstract
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Problems solved by technology

[0005] In order to overcome the shortcomings and deficiencies such as low enzymolysis efficiency, low fermentable sugar concentration, and high production cost in the prior art of lignocellulose enzymatic hydrolysis technology, the primary purpose of the present invention is to provide a kind of anionic surfactant and cationic surfactant A method for improving lignocellulose enzymatic hydrolysis and saccharification yield by compounding as enzymatic hydrolysis auxiliary agent

Method used

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  • Method for improving enzymolysis saccharifing yield of lignocellulose

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Experimental program
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Effect test

Embodiment 1

[0033] Get 100 parts by mass of PH101 microcrystalline cellulose, add it to 5000 parts by mass of pH 4.5, ionic strength is 25mmol / L of acetic acid-sodium acetate buffer solution, add 0.05 parts by mass of sodium lignosulfonate and 0.001 parts by mass of phenyl triphenyl Methyl ammonium bromide, then add 5 FPU / g cellulase based on the content of dextran in the cellulose, and react at a temperature of 50° C. for 72 hours. After the reaction, the cellulose hydrolyzate was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer. The statistical results are shown in Table 1.

Embodiment 2

[0035] Get 100 mass parts of eucalyptus pretreated by dilute acid method, join 2500 mass parts pH is 6.0, in the phosphate buffer solution that ionic strength is 100mmol / L, add 10 mass parts sodium lauryl sulfate and 1 mass part Decyltrimethylammonium bromide, and then add 5 FPU / g cellulase based on the glucan content in lignocellulose, and react at 40° C. for 72 hours. After the reaction, the lignocellulose hydrolyzate was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer. The statistical results are shown in Table 1.

Embodiment 3

[0037] Get 100 mass parts of dilute acid pretreated pine wood, join 2500 mass parts pH and be 5.0, ionic strength is in the acetic acid-sodium acetate buffer solution of 50mmol / L, add 100 mass parts sodium dodecylbenzenesulfonate and 100 parts by mass of dodecyltrimethylammonium chloride, and then adding 10 FPU / g of cellulase based on the dextran content in lignocellulose, and reacting at 50° C. for 72 hours. After the reaction, the lignocellulose hydrolyzate was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer. The statistical results are shown in Table 1.

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Abstract

The invention belongs to the technical field of lignocellulose enzymolysis, and discloses a method for improving the enzymolysis saccharifing yield of lignocellulose by taking the compounded anionic surfactant and cationic surfactant as an enzymolysis auxiliary. According to the method, the compounded anionic surfactant and cationic surfactant is taken as the enzymolysis auxiliary for carrying out enzymolysis on the lignocellulose. The compounded anionic surfactant and cationic surfactant are taken as the enzymolysis auxiliary for improving the enzymolysis saccharifing yield of lignocellulose; by adding 0.05% of the anionic surfactant and the 0.001% of the cationic surfactant, the saccharifing yield can be improved by 44-115%. Meanwhile, the waste liquid obtained by pretreating the pulping and papermaking by-products, i.e., lignosulfonate and lignocellulose, by adopting acid accumulator sulfite can be directly taken as the cationic surfactant for use, therefore, the step of separating the lignocellulose base from pretreatment liquid is saved, the pretreatment liquid is changed into the useful material, and the comprehensive utilization of the lignocellulose resource is realized.

Description

technical field [0001] The invention belongs to the technical field of lignocellulose enzymatic hydrolysis, and in particular relates to a method for improving lignocellulose enzymatic hydrolysis and saccharification yield by using the combination of anionic surfactant and cationic surfactant as an enzymatic hydrolysis auxiliary agent. Background technique [0002] Petroleum resources are increasingly depleted, and the research on the production of biofuels and chemicals from lignocellulosic raw materials has become a research hotspot in recent years. Because lignocellulose has the characteristics of rich sources, low price, and strong regionality, in order to avoid competing with others for food, lignocellulose will become the most potential raw material for bioethanol production. The production of bioethanol from lignocellulose usually includes pretreatment, enzymatic hydrolysis, fermentation, distillation and other processes. However, the process is currently facing many...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCC12P7/10C12P19/02C12P19/14Y02E50/10
Inventor 邱学青楼宏铭林绪亮杨东杰周明松
Owner SOUTH CHINA UNIV OF TECH
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