A method for improving lignocellulose enzymatic saccharification yield

A technology of lignocellulase and lignocellulose, which is applied in the directions of biofuel and fermentation, can solve the problems of low fermentable sugar concentration, low enzymatic hydrolysis efficiency and high production cost, achieve rich sources and improve enzymatic hydrolysis and saccharification yield , the effect of price renewable

Active Publication Date: 2019-10-18
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

[0005] In order to overcome the shortcomings and deficiencies such as low enzymolysis efficiency, low fermentable sugar concentration, and high production cost in the prior art of lignocellulose enzymatic hydrolysis technology, the primary purpose of the present invention is to provide a kind of anionic surfactant and cationic surfactant A method for improving lignocellulose enzymatic hydrolysis and saccharification yield by compounding as enzymatic hydrolysis auxiliary agent

Method used

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  • A method for improving lignocellulose enzymatic saccharification yield

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Embodiment 1

[0033] Take 100 parts by mass of PH101 microcrystalline cellulose and add it to 5000 parts by mass of an acetic acid-sodium acetate buffer solution with a pH of 4.5 and an ionic strength of 25 mmol / L. Add 0.05 parts by mass of sodium lignosulfonate and 0.001 parts by mass of phenyltris Methyl ammonium bromide, and then add 5FPU / g cellulase based on the content of dextran in the cellulose, and react at 50°C for 72h. After the reaction, the cellulose hydrolyzate was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer. The statistical results are shown in Table 1.

Embodiment 2

[0035] Take 100 parts by mass of eucalyptus pretreated by dilute acid method, add 2500 parts by mass of phosphate buffer solution with pH 6.0 and ionic strength of 100mmol / L, add 10 parts by mass of sodium lauryl sulfate and 1 part by mass Decyltrimethylammonium bromide, and then add 5FPU / g cellulase based on the glucan content in lignocellulose, and react at 40°C for 72h. After the reaction, the lignocellulose hydrolysate was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer. The statistical results are shown in Table 1.

Embodiment 3

[0037] Take 100 parts by mass of pine wood pretreated by the dilute acid method and add it to 2500 parts by mass of acetic acid-sodium acetate buffer solution with a pH of 5.0 and an ionic strength of 50 mmol / L, and add 100 parts by mass of sodium dodecylbenzene sulfonate and 100 parts by mass of dodecyltrimethylammonium chloride, and then add 10FPU / g cellulase based on the glucan content in lignocellulose, and react at 50°C for 72h. After the reaction, the lignocellulose hydrolysate was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer. The statistical results are shown in Table 1.

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Abstract

Provided is a method for improving an enzymatic saccharification yield of lignocellulose. The method comprises: adding an anionic surfactant at a concentration as low as 0.5‰ and a cationic surfactant at a concentration of 0.01‰, thereby increasing a saccharification yield to 44-115%. The anionic surfactant is selected from ligin sulfonate and bisulfite of lignocellulose, both being pulp and paper byproducts.

Description

Technical field [0001] The invention belongs to the technical field of lignocellulose enzymatic hydrolysis, and particularly relates to a method for improving the yield of lignocellulose enzymatic hydrolysis and saccharification by using a combination of an anionic surfactant and a cationic surfactant as an enzymatic hydrolysis assistant. Background technique [0002] Petroleum resources are increasingly depleted, and the use of lignocellulose raw materials to produce biofuels and chemicals has become a research focus in recent years. Because lignocellulose has the characteristics of abundant source, low price and strong regionality, in order to avoid competing for food with people, lignocellulose will become the most potential raw material for bioethanol production. The production of bioethanol from lignocellulose usually includes processes such as pretreatment, enzymatic hydrolysis, fermentation, and distillation. However, the process is currently facing many bottlenecks, such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCC12P7/10C12P19/02C12P19/14Y02E50/10
Inventor 邱学青楼宏铭林绪亮杨东杰周明松
Owner SOUTH CHINA UNIV OF TECH
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