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Method for increasing xylan enzymatic saccharification yield

A technology of xylanase and xylan, applied in the direction of fermentation, can solve the problems of high price, unfavorable safe production, easy foaming, etc., and achieves a price that is renewable, beneficial to safe production, and improved saccharification yield. Effect

Active Publication Date: 2015-07-29
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Few scholars have been involved in the technology of improving xylan enzymatic hydrolysis through additives. In addition, the nonionic surfactants used in the above researches, such as PEG, Tween, etc., and bovine serum protein are expensive, which is not conducive to reducing the yield of biomass enzymatic hydrolysis products. Production costs, and enzymatic hydrolysis aids such as PEG and Tween are prone to foaming during use, which is not conducive to safe production in the industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Get 100 mass parts of pure xylan (Aladdin Reagent Shanghai Co., Ltd.), join 5000 mass parts pH is 5.0, in the acetic acid-sodium acetate buffer solution that ionic strength is 50mmol / L, add 25 mass parts molecular weight less than 2500Da woody Sodium sulfonate (provided by Jilin Paper Group) and 0.5 parts by mass of n-decyl alcohol, then add 1mgProtein / g (DW) xylanase in the amount of xylan, react at 50°C for 72h, so as not to add lignin Sodium sulfonate and n-decyl alcohol, other components and the same amount of enzymatic hydrolysis system were used as blank comparison. After the reaction, the hydrolyzate of sugar compounds was obtained by solid-liquid separation, and the xylose content was measured by high-performance liquid chromatography. The results showed that the enzymatic hydrolysis system with sodium lignosulfonate and n-decyl alcohol had a higher saccharification yield than the blank comparison 15.5% (increased from 48.3% of the blank to 63.8%). The reaction ...

Embodiment 2

[0040] Get 100 mass parts of pure xylan, join 5000 mass parts of pH is 5.0, in the acetic acid-sodium acetate buffer solution that ionic strength is 50mmol / L, add 25 mass parts of molecular weight and be the sodium lignosulfonate of 2500~10000Da and 1 Parts by mass of n-octanol, then add 1mgProtein / g (DW) xylanase based on the amount of xylan, react at a temperature of 50°C for 72h, so as not to add sodium lignosulfonate and n-octanol, other components And the enzymatic hydrolysis system with the same amount was used as a blank comparison. After the reaction, the hydrolyzate of sugar compounds was obtained by solid-liquid separation, and the xylose content was measured by high-performance liquid chromatography. The results showed that the enzymatic hydrolysis system with sodium lignosulfonate and n-octanol had a higher saccharification yield than the blank comparison 14.2% (increased from 48.3% of the blank to 62.5%). The reaction phenomena and results are shown in Table 1. ...

Embodiment 3

[0042] Get 100 mass parts of pure xylan, join 5000 mass parts pH is 5.0, in the acetic acid-sodium acetate buffer solution that ionic strength is 50mmol / L, add 25 mass parts of sodium lignosulfonate and 1 mass part of molecular weight less than 2500Da n-octanol, then add 1mgProtein / g(DW) xylanase in terms of xylan amount, react at 50°C for 72h, so as not to add sodium lignosulfonate and n-octanol, other components and dosage The same enzymatic hydrolysis system was used as a blank comparison. After the reaction, the hydrolyzate of sugar compounds was obtained by solid-liquid separation, and the xylose content was measured by high-performance liquid chromatography. The results showed that the enzymatic hydrolysis system with sodium lignosulfonate and n-octanol had a higher saccharification yield than the blank comparison 15.1% (increased from 48.3% of the blank to 63.4%). The reaction phenomena and results are shown in Table 1.

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Abstract

The invention belongs to the technical field of lignocellulose enzymolysis and discloses a method for increasing the xylan enzymatic saccharification yield by taking lignosulfonate and long chain fatty alcohol as an enzymolysis additive. The method comprises the following steps: xylan to be enzymed is prepared to be an enzymatic hydrolysate, the long chain fatty alcohol with the xylan mass of 0.5 to 100 percent and the lignosulfonate with the xylan mass of 0.5 to 100 percent are added, then xylanase is added for enzymolysis reaction, and saccharide hydrolysate after enzymolysis is obtained. According to the method provided by the invention, firstly, the lignosulfonate and the long chain fatty alcohol are compounded to serve as an enzymolysis additive, due to the compounding of the lignosulfonate and the long chain fatty alcohol, the lignosulfonate and the long chain fatty alcohol together promote the enzymolysis reaction of the xylanase, and the saccharification yield of the xylan is increased by 9.2 to 15.5 percent. The lignosulfonate and the long chain fatty alcohol, used in the method provided by the invention, are rich in sources, low in prices and are renewable, and are environment-friendly additives. According to the method provided by the invention, during the enzymolysis process, no foams are produced, and the method provided by the invention is safe and efficient.

Description

technical field [0001] The invention belongs to the technical field of lignocellulose enzymatic hydrolysis, and in particular relates to a method for improving the yield of xylan enzymatic hydrolysis and saccharification by using lignosulfonate and long-chain fatty alcohol as enzymatic hydrolysis aids. Background technique [0002] With the growth of the world population and the development of the economy, the human demand for energy is increasing day by day. The excessive use of fossil resources has caused human beings to fall into an energy crisis and serious environmental pollution problems. Finding alternatives to non-renewable resources has become a major issue that human society must face for survival and sustainable development. Starting from the environmentally friendly, renewable, and abundant biomass resources, the production of biofuels and high value-added chemicals has become a research hotspot. [0003] As an important renewable biomass resource, xylose is the...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
Inventor 邱学青袁龙楼宏铭杨东杰
Owner SOUTH CHINA UNIV OF TECH
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