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Method for improving cellulose enzymatic hydrolysis and saccharification yield

A technology of cellulase and cellulose, which is applied in the direction of fermentation, etc., can solve the problems of high production cost, low efficiency of cellulose enzymatic hydrolysis, and low concentration of fermentable sugar, so as to achieve higher saccharification yield, rich sources, and lower production costs Effect

Active Publication Date: 2015-07-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problems of low cellulose enzymatic hydrolysis efficiency, low fermentable sugar concentration and high production cost in the current enzymatic hydrolysis process, the purpose of the present invention is to provide a kind of lignosulfonate with rich sources, low price and renewable The compound mixture of straight-chain alcohols is used as an enzymatic hydrolysis aid to improve the yield of cellulose enzymatic hydrolysis and saccharification;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Get 100 mass parts of Whatman No. 1 filter paper, join 5000 mass parts of pH is 4.8, in the acetic acid-sodium acetate buffer solution that ionic strength is 50mmol / L, add 25 mass parts of molecular weight and be the sodium lignosulfonate of 2500~10000Da and 0.5 mass parts 1 part of n-dodecyl alcohol, then add 10 FPU / g of cellulase based on the content of dextran in the cellulose, and react at a temperature of 50° C. for 72 hours. After the reaction, the hydrolyzate of carbohydrate compounds was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer, and the increase in saccharification yield was 24.6%. The statistical results are shown in Table 1.

Embodiment 2

[0035] Get 100 parts by mass of microcrystalline cellulose, add 5000 parts by mass of pH to 4.8, ionic strength is 50mmol / L acetic acid-sodium acetate buffer solution, add 25 parts by mass of sodium lignosulfonate with a molecular weight of 10000-50000Da and 50 Parts by mass of n-octanol, and then adding 10 FPU / g of cellulase based on the content of dextran in the cellulose, and reacting at a temperature of 50° C. for 72 hours. After the reaction, the hydrolyzate of carbohydrate compounds was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer, and the increase in saccharification yield was 17.0%. The statistical results are shown in Table 1.

Embodiment 3

[0037] Get 100 mass parts of microcrystalline cellulose, join 5000 mass parts of pH is 4.8, in the acetic acid-sodium acetate buffer solution that ionic strength is 50mmol / L, add 50 mass parts of molecular weight and be that sodium lignosulfonate of 10000~50000Da and 1 Parts by mass of n-octanol, and then adding 10 FPU / g of cellulase based on the content of dextran in the cellulose, and reacting at a temperature of 50° C. for 72 hours. After the reaction, the hydrolyzate of carbohydrate compounds was obtained by solid-liquid separation, and the glucose content was measured by a biosensor analyzer, and the increase in saccharification yield was 15.1%. The statistical results are shown in Table 1.

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PUM

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Abstract

The invention belongs to the field of lignocellulose enzymatic hydrolysis, and discloses a method for improving cellulose enzymatic hydrolysis and saccharification yield by using a mixture of lignin sulfonate and straight-chain alcohol as an enzymatic hydrolysis auxiliary agent. The method comprises the specific steps that: cellulose is dispersed in a buffering solution; the mixture of lignin sulfonate and straight-chain alcohol is added; 5-20 FPU / g of cellulase calculated according to glucan content in cellulose is added; a reaction is allowed for 48-96h under a temperature of 45-60 DEG C, such that a saccharide compound hydrolysate is obtained. According to the invention, through the addition of the mixture of lignin sulfonate and straight-chain alcohol, cellulose enzymatic hydrolysis and saccharification yield can be improved by 15.1-24.6%.

Description

technical field [0001] The invention belongs to the field of enzymatic hydrolysis of lignocellulose, and in particular relates to a method for improving the yield of enzymatic hydrolysis and saccharification of cellulose by mixing lignosulfonate and straight-chain alcohol as enzymatic hydrolysis aids. Background technique [0002] With the growth of the world population and the development of the economy, the human demand for energy is increasing day by day. Cellulosic ethanol can effectively solve the problems of energy shortage, resource scarcity and environmental pollution. It is the first choice of alternative fuel and the main development direction of biofuel. [0003] The current research hotspot is to use enzymatic hydrolysis technology to hydrolyze lignocellulose to generate bioethanol. The process mainly includes raw material pretreatment, cellulose enzymatic hydrolysis, sugar fermentation and distillation. However, the process is currently facing many bottlenecks,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/08C12P19/14
Inventor 邱学青楼宏铭赖焕然杨东杰周海峰庞煜霞欧阳新平周明松
Owner SOUTH CHINA UNIV OF TECH
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