Method for measuring urushiol in sumac fruit oil by UPLC-MS

A technology of salt skin wood and fruit oil, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unclear vision, insufficient sensitivity, light color rendering, etc., to reduce interference, optimize mass spectrometry conditions, and reduce impurity signals Effect

Inactive Publication Date: 2020-01-10
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is most suitable for determining urushiol content of more than 100mg / mL concentration (concentration is too low, too light color, can not see clearly), and its sensitivity is too low, so it is not suitable for analyzing trace amounts of urushiol in Saltwood oil
[0008] High-performance liquid chromatography of urushiol; it can also be used at 225nm. Although it is not the most sensitive line of urushiol, it still has a strong absorption. It is often used for HPLC separation and detection with a UV detector. Saturated urushiol prepared by the preparation method of saturated urushiol in the appendix of the phenol standard national standard "Raw Urushiol" (GB4354-84), that is, 1 to 3 double bonds in the R branch chain in the urushiol structure are saturated with H to form saturated urushiol phenol, but the urushiol in the basil oil is mostly unsaturated urushiol (containing 1 to 3 double bonds in the R branch), and the content of saturated urushiol is less, while the HPLC ultraviolet detector detects that the basil oil is unsaturated. Determination of very small amount of saturated urushiol content in medium, the sensitivity is not high enough

Method used

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  • Method for measuring urushiol in sumac fruit oil by UPLC-MS
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  • Method for measuring urushiol in sumac fruit oil by UPLC-MS

Examples

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Embodiment 1

[0059] Preparation of saturated urushiol standard solution

[0060] Take a 2mL centrifuge tube, wash it three times with methanol, dry it for later use, accurately weigh 1.0mg of saturated urushiol, add 1mL of methanol, shake well, and filter through a 0.22μm microporous membrane to obtain a 1mg / mL saturated urushiol standard solution. Then dilute with methanol to the desired concentration for liquid chromatography-mass spectrometry analysis.

Embodiment 2

[0062] Preparation of Salmonella sample solution

[0063] Squeeze Salmonia fruit oil: After the Salmonella fruit is roasted (200°C, 10-15min), the crude oil squeezed out by a small oil press is stored in a barreled freezer and warmed up before sample preparation (heating and melting) , absorb 1.0mL of the oil, add 1.0mL of methanol to a 5mL centrifuge tube, shake well, extract the polyphenols, let it stand at 4°C overnight, take the supernatant (methanol layer), and use a 0.22μm micropore Membrane filtration, get 1 # sample liquid.

Embodiment 3

[0065] Preparation of Salmonella sample solution

[0066] Ether extraction of sarcocarp oil: use anhydrous ether Soxhlet extraction method (method refers to GB / T 5535.1-2008 Determination of unsaponifiable matter in animal and vegetable oils and fats Part 1: fast method of ether extraction) extracted sarcocarp oil Add 1.0mL of methanol to a 5mL centrifuge tube, add 1.0mL of methanol, shake well, extract the polyphenols, let stand at 4°C overnight, take the supernatant (methanol layer), and filter it with a 0.22μm microporous membrane. got 2 # sample liquid.

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Abstract

The invention discloses a method for measuring urushiol in sumac fruit oil by UPLC-MS, and relates to the technical field of urushiol measurement. The method comprises the following steps of (1) pre-processing a sumac fruit sample; and (2) measuring the sample pre-processed in the step (1) by UPLC-MS. The method has the beneficial effects that saturated urushiol in different sumac samples can be detected by the detection method, the content of a tiny amount of saturated urushiol in the sumac fruit oil is detected, and the content is 27.5-57.5mg / L. and by the detection method, unsaturated urushiol in the sumac samples can be detected, and the total urushiol content in the sumac is 165-345mg / L.

Description

technical field [0001] The invention relates to the technical field of urushiol determination, in particular to a method for determining urushiol in sarcocarpus oil by using UPLC-MS. Background technique [0002] Both the fruit and its seeds are rich in oil. Preliminary studies have shown that the average oil content in the wild fruit is as high as 18%, of which unsaturated fatty acids are the main content, and the content can reach 72%, which is very useful for development and application. value. [0003] However, Rhus chinensis Mill (Rhus chinensis Mill) belongs to the Rhus chinensis genus of the Rhus chinensis family, and it belongs to the close relative species of the Rhus chinensis family with the Rhus chinensis. Sensitivity component—— urushiol, is one of the main hidden dangers of the oil of sativa as edible oil. [0004] Urushiol (Urushiol), which accounts for 50% to 75% of its dry weight in raw lacquer, is a kind of hydrocarbon-based phenolic compound with catecho...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/7266
Inventor闵运江陈存武邓辉许正刚闵长莉汪学军王广林陈乃东乔德亮陈翰
OwnerWEST ANHUI UNIV