Livestock-and-poultry-breeding wastewater treatment agent and application method thereof
A livestock and poultry breeding wastewater and water treatment agent technology, applied in the field of livestock and poultry breeding, can solve problems such as difficult to remove, difficult to separate, and small volume of solids, and achieve the effects of uniform shape and weight, improved tolerance, and increased value
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[0029] According to one embodiment of the present invention, the preparation method of bacterial algal balls is:
[0030] Step 1: Prepare BG-11 medium containing 0.6-1.0g / mL yeast extract, 3.0-5.0g / mL starch and 1.0-10.0mg / L calcium malate, adjust the pH to 5.5-7.0, and sterilize at 121°C 20min, cooling, standby;
[0031] Step 2: Introduce cells into BG-11 medium at a density of 1.0×10 5 -10 7cell / mL microalgae liquid and edible fungus hyphae, cultivated for 5-10 days under the condition of rotating speed of 150-180r / min and temperature of 25-30°C to obtain bacterial algal balls; among them, every 100mL of BG-11 cultured Base, the volume of microalgae liquid is 8-15mL, the inoculum size of edible fungus mycelium is 4-6cm 2 .
[0032] According to one embodiment of the present invention, the microalgae is at least one selected from the group consisting of Anabaena blooms, Scenedesmus, Spirulina, and Chlorella. The selection of microalgae should comprehensively consider the...
Embodiment 1
[0042] The livestock and poultry breeding wastewater treatment agent comprises bacterium algae balls, and the preparation method of bacterium algae balls is as follows:
[0043] Step 1: Prepare BG-11 medium containing 1.0g / mL yeast extract, 5.0g / mL starch and 10.0mg / L calcium malate, adjust the pH to 7.0, sterilize at 121°C for 20min, cool, and set aside;
[0044] Step 2: Introduce cells into BG-11 medium at a density of 1.0×10 7 cell / mL Scenedesmus algae liquid and Pleurotus ostreatus mycelium, cultivated for 10 days under the conditions of rotating speed of 180r / min and temperature of 30°C to obtain bacteria and algae balls; among them, for every 100mL of BG-11 medium, the amount of microalgae algae liquid The volume is 15mL, and the inoculum size of edible fungus mycelium is 6cm 2 .
Embodiment 2
[0046] The livestock and poultry breeding wastewater treatment agent comprises bacterium algae balls, and the preparation method of bacterium algae balls is as follows:
[0047] Step 1: Prepare BG-11 medium containing 0.76g / mL yeast extract, 4.2g / mL starch and 3.5mg / L calcium malate, adjust the pH to 6.5, sterilize at 121°C for 20min, cool, and set aside;
[0048] Step 2: Introduce cells into BG-11 medium at a density of 1.0×10 6 cell / mL chlorella liquid and shiitake mushroom hyphae, cultivated for 7 days under the conditions of rotating speed of 160r / min and temperature of 28°C to obtain bacterial algae balls; among them, for every 100mL of BG-11 medium, microalgae liquid The volume is 11mL, and the inoculum size of edible fungus mycelium is 4.5cm 2 .
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