Livestock-and-poultry-breeding wastewater treatment agent and application method thereof

A livestock and poultry breeding wastewater and water treatment agent technology, applied in the field of livestock and poultry breeding, can solve problems such as difficult to remove, difficult to separate, and small volume of solids, and achieve the effects of uniform shape and weight, improved tolerance, and increased value

Inactive Publication Date: 2020-01-14
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, livestock and poultry breeding wastewater is a high-concentration organic wastewater that is difficult to treat, mainly because of its large discharge volume, low temperature, mixed solids and liquids in the wastewater and small volume of solids, which makes it difficul...

Method used

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  • Livestock-and-poultry-breeding wastewater treatment agent and application method thereof
  • Livestock-and-poultry-breeding wastewater treatment agent and application method thereof
  • Livestock-and-poultry-breeding wastewater treatment agent and application method thereof

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Embodiment approach

[0029] According to one embodiment of the present invention, the preparation method of bacterial algal balls is:

[0030] Step 1: Prepare BG-11 medium containing 0.6-1.0g / mL yeast extract, 3.0-5.0g / mL starch and 1.0-10.0mg / L calcium malate, adjust the pH to 5.5-7.0, and sterilize at 121°C 20min, cooling, standby;

[0031] Step 2: Introduce cells into BG-11 medium at a density of 1.0×10 5 -10 7cell / mL microalgae liquid and edible fungus hyphae, cultivated for 5-10 days under the condition of rotating speed of 150-180r / min and temperature of 25-30°C to obtain bacterial algal balls; among them, every 100mL of BG-11 cultured Base, the volume of microalgae liquid is 8-15mL, the inoculum size of edible fungus mycelium is 4-6cm 2 .

[0032] According to one embodiment of the present invention, the microalgae is at least one selected from the group consisting of Anabaena blooms, Scenedesmus, Spirulina, and Chlorella. The selection of microalgae should comprehensively consider the...

Embodiment 1

[0042] The livestock and poultry breeding wastewater treatment agent comprises bacterium algae balls, and the preparation method of bacterium algae balls is as follows:

[0043] Step 1: Prepare BG-11 medium containing 1.0g / mL yeast extract, 5.0g / mL starch and 10.0mg / L calcium malate, adjust the pH to 7.0, sterilize at 121°C for 20min, cool, and set aside;

[0044] Step 2: Introduce cells into BG-11 medium at a density of 1.0×10 7 cell / mL Scenedesmus algae liquid and Pleurotus ostreatus mycelium, cultivated for 10 days under the conditions of rotating speed of 180r / min and temperature of 30°C to obtain bacteria and algae balls; among them, for every 100mL of BG-11 medium, the amount of microalgae algae liquid The volume is 15mL, and the inoculum size of edible fungus mycelium is 6cm 2 .

Embodiment 2

[0046] The livestock and poultry breeding wastewater treatment agent comprises bacterium algae balls, and the preparation method of bacterium algae balls is as follows:

[0047] Step 1: Prepare BG-11 medium containing 0.76g / mL yeast extract, 4.2g / mL starch and 3.5mg / L calcium malate, adjust the pH to 6.5, sterilize at 121°C for 20min, cool, and set aside;

[0048] Step 2: Introduce cells into BG-11 medium at a density of 1.0×10 6 cell / mL chlorella liquid and shiitake mushroom hyphae, cultivated for 7 days under the conditions of rotating speed of 160r / min and temperature of 28°C to obtain bacterial algae balls; among them, for every 100mL of BG-11 medium, microalgae liquid The volume is 11mL, and the inoculum size of edible fungus mycelium is 4.5cm 2 .

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Abstract

The invention provides a livestock-and-poultry-breeding wastewater treatment agent and an application method thereof, and belongs to the technical field of livestock and poultry breeding. The livestock-and-poultry-breeding wastewater treatment agent comprises bacteria-algae balls, pyruvic acid and beta-glucan, wherein microalgae and edible fungus mycelium are inoculated to a BG-11 culture medium which contains a yeast extract, starch and calcium malate, and co-culture is performed for 5-10 days so as to prepare the bacteria-algae balls. When the livestock-and-poultry-breeding wastewater treatment agent is applied to treatment of livestock-poultry breeding wastewater, at least one of the following effects can be achieved that expression of chlorophyll genes in algae cells can be enhanced, the enzyme activity of SOD and/or CAT and/or POD of microalgae can be improved, the tolerance of microalgae to antibiotics is improved, antibiotics, TN, TP, NH<4><+>-N and COD in the livestock-poultrybreeding wastewater can be removed through the symbiotic metabolism of bacteria and algae, and accumulation of oil and starch in microalgae is enhanced; and the application method of the livestock-and-poultry breeding wastewater treatment agent comprises the step of putting the wastewater treatment agent into the livestock-poultry breeding wastewater.

Description

technical field [0001] The invention belongs to the technical field of livestock and poultry breeding, and in particular relates to a treatment agent for livestock and poultry breeding wastewater and a method for using the same. Background technique [0002] In recent years, the domestic livestock and poultry breeding industry has shown a trend of rapid development, and the livestock and poultry breeding industry has gradually become the most active growth point and the main pillar industry in my country's agricultural economy. The livestock and poultry breeding industry is an important part of my country's agriculture and rural economy, and it is also one of the main causes of non-point source pollution in my country's rural areas. At present, intensive farms do not have a sufficient amount of supporting cultivated land to absorb the livestock and poultry excrement they produce. Many farms pile up everywhere, and sewage is discharged randomly, causing serious pollution of a...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F3/34C02F103/20
CPCC02F3/325C02F3/341C02F3/342C02F2103/20
Inventor 汤江武孙宏姚晓红沈琦王新吴逸飞李园成
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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