Method for extracting selenium polypeptide from selenium-rich passion fruit seeds

A technology of passion fruit seeds and passion fruit, which is applied in the field of polypeptide extraction, can solve the problems of waste of selenium-enriched passion fruit seeds, and achieve the effects of saving repeated protein extraction steps, avoiding the loss of selenium polypeptides, and reducing losses

Active Publication Date: 2020-01-17
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is: for the low utilization rate of selenium-enriched passion fruit seed causes the problem of waste, the present invention proposes a kind of method for extracting selenium polypeptide from selenium-enriched passion fruit seed, this method can quickly extract passion fruit seed Compared with traditional methods, the selenium polypeptides are extracted and utilized. Compared with traditional methods, the operation is simpler, the cost is lower, and the extraction recovery rate is high. The extracted selenium polypeptides are of good quality and have significant economic value.

Method used

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  • Method for extracting selenium polypeptide from selenium-rich passion fruit seeds
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  • Method for extracting selenium polypeptide from selenium-rich passion fruit seeds

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Embodiment approach

[0028] A method for extracting selenium polypeptide from selenium-enriched passion fruit seeds, the following is one or more preferred embodiments, and its specific steps are as follows:

[0029] 1. Take the selenium-enriched passion fruit, cut it in half with a knife, dig out the pulp with seeds, use a three-legged centrifuge, filter with a density of 20 mesh, and centrifuge at a speed of 5000r / min for 5min. Collect the passion fruit seeds inside the strainer.

[0030] 2. Wash the passion fruit seeds with clean water, dry them, crush them with a Chinese herbal medicine grinder, and pass through a 60-mesh sieve to remove most of the hard shells. The sieved powder is added with water according to the ratio of material to liquid 1:10 (g / mL), and homogenized with a colloid mill. The homogenization temperature is 25°C, the gap between the colloid mill is 0.1 mm, and the homogenization time is 5 minutes.

[0031] 3. The homogenized mixture passes through a 200-mesh sieve, and is t...

Embodiment 2

[0037] A method for extracting selenium polypeptide from selenium-enriched passion fruit seeds, the following is one or more preferred embodiments, and its specific steps are as follows:

[0038]1. Take the selenium-enriched passion fruit, cut it in half with a knife, dig out the seed pulp with a pulper (or by hand), use a three-legged centrifuge, filter with a density of 20 mesh, and centrifuge at a speed of 5000r / min for 8 minutes. Collect the passion fruit seeds inside the strainer.

[0039] 2. Wash the passion fruit seeds with clean water, dry them, crush them with a Chinese herbal medicine grinder, and pass through a 60-mesh sieve to remove most of the hard shells. The sieved powder is added with water according to the ratio of material to liquid 1:10 (g / mL), and homogenized with a colloid mill, wherein the homogenization temperature is 25°C, the colloid mill gap is 1 mm, and the homogenization time is 10 minutes.

[0040] 3. The homogenized mixture passes through a 200-...

Embodiment 3

[0046] A method for extracting selenium polypeptide from selenium-enriched passion fruit seeds, the following is one or more preferred embodiments, and its specific steps are as follows:

[0047] 1. Take the selenium-enriched passion fruit, cut it in half with a knife, dig out the pulp with seeds, use a three-legged centrifuge, filter with a density of 20 mesh, and centrifuge at a speed of 5000r / min for 6 minutes. Collect the passion fruit seeds inside the strainer.

[0048] 2. Wash the passion fruit seeds with clean water, dry them, crush them with a Chinese herbal medicine grinder, and pass through a 60-mesh sieve to remove most of the hard shells. The sieved powder is added with water according to the ratio of material to liquid 1:10 (g / mL), and homogenized with a colloid mill. The homogenization temperature is 25°C, the gap between the colloid mill is 0.5mm, and the homogenization time is 8 minutes.

[0049] 3. The homogenized mixture passes through a 200-mesh sieve, and ...

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Abstract

The invention discloses a method for extracting selenium polypeptide from selenium-rich passion fruit seeds. The method comprises the following steps: taking out, cleaning and drying selenium-rich passion fruit seeds, performing crushing and sieving, carrying out ultrasonic treatment, carrying out ultrasonic action to separate grease in a mixed solution, centrifugally removing oil, performing precipitating to obtain a protein mixture in the passion fruit seeds, performing homogenizing to turn proteins into a stable system, carrying out enzymolysis by using an immobilized enzyme, and carrying out centrifugal separation to obtain the purified selenium-containing polypeptide mixture of the passion fruit seeds. According to the method, the selenium polypeptide in the passion fruit seeds can berapidly extracted and utilized. Compared with traditional methods, the method has the advantages of easy operation, low cost and high extraction recovery rate, and the extracted selenium polypeptideis good in quality and remarkable in economic value effect.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a method for extracting selenium polypeptides from selenium-enriched passion fruit seeds. Background technique [0002] Passion fruit is a herbaceous vine of the Passiflora family Passiflora, and the fruit can be eaten raw or used as a vegetable. Being used as medicine has the effect of excitement and strength. The fruit pulp is juicy, which can be made into an aromatic and delicious drink, and can also be added to other drinks to improve the quality of the drink. The seeds are pressed for oil, which can be used for food, soap making, paint making and so on. Passion fruit contains 17 kinds of amino acids, rich in protein, fat, sugar, vitamins, calcium, phosphorus, iron, potassium and other substances beneficial to the human body. It is also known as the king of VC (vitamins) in fruits because of its unique taste , sweet and sour, has the functions of detoxificat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30
CPCC12P21/06C07K1/36C07K1/34C07K1/30
Inventor 游向荣周葵李明娟王颖张雅媛卫萍
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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