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Screening method of plant-derived immunoenhancement type feed additive for eels

A feed additive and immune enhancement technology, applied in biochemical equipment and methods, cell dissociation methods, blood/immune system cells, etc., can solve problems such as different physiological and biochemical effects, and achieve easy-to-operate effects

Pending Publication Date: 2020-01-21
JIMEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, different plant source extracts, especially different compound formulations, have different physiological and biochemical effects on different animals

Method used

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  • Screening method of plant-derived immunoenhancement type feed additive for eels
  • Screening method of plant-derived immunoenhancement type feed additive for eels
  • Screening method of plant-derived immunoenhancement type feed additive for eels

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Experimental program
Comparison scheme
Effect test

Embodiment

[0053] Utilize the method of the present invention to screen the following five kinds of plant source compound, respectively polysaccharide (Dt) compound, Pulsatilla (Btw) compound, Radix Radix (Blg) compound, rhubarb (Dh) compound, thymol (Blf) compound.

[0054] 1. Screening test:

[0055] Screening and comparison of 5 kinds of plant-derived compounds (Dt / Blg / Btw / Dh / Blf): Taking the non-added group as the control, the NBT% of each compound increased to varying degrees, indicating that it can promote the respiratory burst activity of white blood cells. Blg group, Dt group, Btw group, Blf group, Dh group have improved respectively 1030%, 728%, 413%, 219%, 121% (specifically refer to figure 1 ), Blg group>Dt group>Btw group>Blf group>Dh group, so the significantly improved Dt / Blg / Btw group is selected as an alternative prescription.

[0056] Furthermore, by flow cytometry, the leukocytes were co-incubated with the three plant-derived compound Dt / Btw / Blg, compared with the non-...

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Abstract

The invention provides a screening method of plant-derived immunoenhancement type feed additive for eels. The screening method comprises steps as follows: preparing a plant-derived extract liquid, andthen, preparing an eel plasma leukocyte solution by a discontinuous density gradient centrifugation method; determining respiratory burst activity and phagocytosis vitality of plasma leukocytes by anELIASA and a flow cytometer respectively, and the activation effect of the plant-derived extract on plasma leukocytes of eels is evaluated in combination of a computation formula. The screening method can complete cell vitality effect tests at high, medium and low concentrations of multiple plant-derived liquids simultaneously at one time, and provides efficient screening technical reference forapplication of the plant-derived extract in aquaculture and fish drug development.

Description

【Technical field】 [0001] The invention relates to a screening method for a functional feed additive, in particular to a screening method for a botanical immune-enhancing feed additive for eels. 【Background technique】 [0002] Plant source extracts are more and more widely used in aquaculture production, because they can be used as green substitutes for antibiotics and chemically synthesized fish additives, and have immune enhancement, anti-inflammatory, food-attracting, digestion-promoting and growth-promoting properties for farmed animals. And many other effects. Many plant extracts have the effect of improving the specific immunity of animals, such as the respiratory burst activity and phagocytosis activity of plasma leukocytes, which can enhance the ability of animals to resist disease infection, and can effectively prevent disease outbreaks as feed additives. However, different plant source extracts, especially different compound formulations, have different physiologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12N5/078
CPCC12N5/0634G01N33/5047C12N2509/10
Inventor 李忠琴翟少伟杨求华江兴龙
Owner JIMEI UNIV
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