Preparation method of protein liposome FNL and application thereof in cosmetics

A technology of liposome and protein lipid, which is applied in the field of preparation of fibronectin liposome, can solve the problems of inability to absorb the skin and achieve the effect, so as to avoid degradation and deterioration, promote absorption and utilization, and transdermal absorption rate high effect

Inactive Publication Date: 2020-01-24
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a high-molecular glycoprotein, FN cannot be absorbed by the skin when directly added and used, and cannot achieve the desired effect

Method used

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  • Preparation method of protein liposome FNL and application thereof in cosmetics
  • Preparation method of protein liposome FNL and application thereof in cosmetics
  • Preparation method of protein liposome FNL and application thereof in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, a kind of preparation method of proteoliposome FNL

[0024] S1 collects fresh animal blood, adds 3.8% sodium citrate for anticoagulation (9:1 volume ratio), centrifuges at 1500 rpm for 15 min, and collects the upper plasma. Add ammonium sulfate to the collected plasma to a saturation of 30%, let it stand for 2 hours, filter to collect the precipitate and dissolve it again, separate and extract with gelatin-agarose 2B affinity chromatography column, Sephadex G75 Molecular sieve purification, get fibronectin solution I after degerming;

[0025] S2 Dissolve hydrogenated lecithin, cholesterol and vitamin E in an appropriate amount of chloroform, transfer the solution to a spinner bottle, and remove the chloroform by rotary evaporation at 20-30°C and 100-200r / min to form a uniform film; The solid-liquid ratio of hydrogenated lecithin, cholesterol, vitamin E and chloroform is 5g:2g:1g:200ml;

[0026] S3 takes 0.1-10 mg of fibronectin collected in step S1 and d...

Embodiment 2

[0028] Embodiment 2, a kind of preparation method of proteoliposome FNL

[0029] S1 Collect 900 mL of fresh animal blood, add 100 mL of 3.8% sodium citrate for anticoagulation, centrifuge at 1500 rpm for 15 min, and collect the upper plasma. Add ammonium sulfate to the collected plasma to a saturation of 30%, let it stand for 2 hours, filter to collect the precipitate and dissolve it again, separate and extract with gelatin-agarose 2B affinity chromatography column, Sephadex G75 Molecular sieve purification, get fibronectin solution I after degerming;

[0030] S2 Dissolve 500 mg of hydrogenated lecithin, 200 mg of cholesterol, and 100 mg of vitamin E in 20 mL of chloroform, transfer the solution to a spinner bottle, and remove the chloroform by rotary evaporation at 25°C and 140 r / min to form a uniform film;

[0031] S3 Take 0.5 mg of fibronectin collected in step S1 and dissolve it in 10 mL of phosphate buffer (0.1 M, pH 6.5), add 0.1% bovine serum albumin solution, mix well...

Embodiment 3

[0033] Embodiment 3, a kind of sunscreen containing fibronectin liposome

[0034] The formula of described sunscreen is as shown in table 1:

[0035] Table 1 A kind of sunscreen of proteoliposome FNL

[0036]

[0037]

[0038] According to the formula in Table 1: heat A1 and A2 separately to 60°C, dissolve them all and keep warm; uniformly disperse titanium dioxide in deionized water with an ultrasonic disperser and heat to 60°C to keep warm, and add the remaining ingredients of phase B while stirring. After ensuring complete dissolution, add phase B to the mixed (A1+A2) phase for emulsification under stirring; then add phase C, cool to room temperature, stop stirring, and discharge to obtain a sample.

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Abstract

The invention belongs to the field of biomedicine and cosmetics, and specifically relates to the technical field of liposome preparation, in particular to a preparation method of animal blood fibronectin liposome and application of the obtained product. In the invention, animal blood fibronectin is added to hydrogenated lecithin, cholesterol and other materials to make protein liposome FNL. The fibronectin encapsulated by the liposome is applied to skin care products and can be absorbed by the skin more quickly. After absorption through the skin, multiple effects of whitening, anti-aging, moisturizing, repairing, etc. can be better achieved, thus showing a good application prospect for fibronectin to enter high-end cosmetics.

Description

technical field [0001] The invention relates to the field of biomedicine and cosmetics, in particular to the technical field of liposome preparation, in particular to a preparation method of fibronectin liposome and the use of the obtained product. Background technique [0002] Fibronectin (FN) is a high-molecular-weight glycoprotein widely present in blood, body fluids and various tissues, with a molecular mass of 450ku, which is connected by two subunits with a molecular mass of 220ku through an interchain disulfide bond. made. Its subunit has 6 dense spheroids, each region has a specific function, can be combined with a special ligand, and has a variety of biological functions. FN is widely involved in the processes of cell migration, adhesion, proliferation, hemostasis and tissue repair, mobilizes the mononuclear phagocyte system to remove harmful substances from damaged tissues, and provides nutrients and information channels for cells. FN can promote the migration of...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/14A61Q17/04A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/64A61K8/14A61Q17/04A61Q19/004A61Q19/00A61Q19/08A61Q19/02A61K2800/82
Inventor 刘忠杨涛
Owner JINAN UNIVERSITY
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