A method for the determination of fatty acids in rice by gas chromatography-mass spectrometry
A gas chromatography and mass spectrometry technology, applied in the field of fatty acid determination, can solve problems such as poor accuracy, complex fatty acid types, and no accurate determination of fatty acid components, and achieve the effect of improving accuracy
Active Publication Date: 2020-12-04
山东国仓健生物科技有限公司
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Problems solved by technology
[0004] Although with the development of high-sensitivity mass spectrometry technology, gas chromatography-mass spectrometry technology has also appeared to measure fatty acids at present, but the current gas chromatography-mass spectrometry technology can only realize the determination of relatively few types of fatty acids. The determination of many and complex fatty acid components has poor accuracy. Due to the complex types of fatty acids in rice and their uneven content, there are few reports on the determination of fatty acid components in rice, let alone how to use gas chromatography-mass spectrometry. Relevant research and reports on the realization of accurate determination of fatty acid components in rice
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[0064] (1) Grind the rice seed sample to pulverize, accurately weigh 0.1 g of the pulverized sample in a 15 mL dry screw glass tube;
[0065] (2) Add 3.0 mL of n-hexane, 3 mL of acetyl chloride-methanol solution with a volume fraction of 10%, and 0.3 mg of dibutylhydroxytoluene into the sample in sequence, and tighten the screw cap of the glass tube;
[0066] (3) After shaking and mixing, place in 80 ℃ water bath for 2h, take out and shake once every 20min during the period, realize fatty acid methylation, take out after water bath and cool to room temperature;
[0067] (4) Transfer the reacted sample solution to a 50mL centrifuge tube, wash the glass tube twice with 3mL sodium carbonate solution, combine the sodium carbonate solution in a 50mL centrifuge tube, shake and mix well, and the concentration of the sodium carbonate solution is 6% ;
[0068] (5) Centrifuge at 4000 r / min for 10 min, suck 1 mL of supernatant through a glass pipette, and pass through a 0.22 μm filter t...
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The invention discloses a method for determining fatty acid in rice by using the gas chromatography-mass spectrometry. The method comprises the following steps: (1) preparation of a sample to be determined: pulverizing a rice sample, sequentially adding n-hexane, an acetyl chloride-methanol solution and butylated hydroxytoluene, mixing, and heating to perform fatty acid extraction and methyl esterification treatment; cooling to room temperature, adding a sodium carbonate solution, uniformly mixing, centrifuging, taking supernatant, and filtering to obtain a sample to be detected; and (2) determination of fatty acid components: performing quantitative or qualitative determination on 37 kinds of fatty acids of the sample obtained to be detected in the step (1) by adopting a gas chromatograph-mass spectrometer. According to the present invention, through the common cooperation of the extraction method and the determination method, the accurate qualitative and quantitative determination of37 kinds of fatty acids in rice is achieved, the difficult problem that the fatty acid components in rice cannot be accurately determined in the prior art is solved, and the determination of a variety of fatty acids can be achieved on the basis of the assurance of the determination accuracy of the fatty acid components.
Description
technical field [0001] The invention relates to the technical field of fatty acid determination, in particular to a method for determining fatty acids in rice by gas chromatography-mass spectrometry. Background technique [0002] Fat is the main source of human energy intake, and more importantly, it can provide the essential fatty acids needed by the human body. Plant fatty acid is an important component of plant nutrients. Its components and content ratio determine the quality, nutritional value and biological effects of vegetable oils. Plant fatty acids are mainly divided into saturated fatty acids and unsaturated fatty acids. The quality of the value plays a crucial role. Therefore, developing a method that can accurately detect plant fatty acids will have a profound impact on the in-depth understanding of the composition of plant fatty acid components, thereby improving the oil content in plants, improving human dietary structure, and promoting human dietary health. ...
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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 胥倩汪勇郎法洋谷淑波王群青马春阳
Owner 山东国仓健生物科技有限公司