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Specific DNA fragment com58276 and application thereof to adjustment and control of stress resistance of plants

A technology of stress resistance and DNA molecules, applied in the direction of recombinant DNA technology, applications, plant peptides, etc., can solve the problems of stress-resistance-related genes and fragments related to stress-resistance, which are rarely studied

Active Publication Date: 2020-02-07
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, the research on Caragana caragana has mainly focused on biological characteristics, physiological changes and anatomical structure, while there are very few studies on stress-resistance-related genes and fragments

Method used

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  • Specific DNA fragment com58276 and application thereof to adjustment and control of stress resistance of plants
  • Specific DNA fragment com58276 and application thereof to adjustment and control of stress resistance of plants
  • Specific DNA fragment com58276 and application thereof to adjustment and control of stress resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Obtaining and drought resistance identification of transgenic com58276 Arabidopsis

[0046] 1. Obtaining the specific DNA fragment com58276

[0047] 1. Sowing Caragana caragana seeds in sandy soil, normal growth management, and obtaining Caragana caragana plants growing for 1 month; then stopping watering for 20 days to obtain Caragana caragana plants treated with drought.

[0048] Caragana caragana plants under drought treatment have well-developed root system, whitish leaves and thicker hairs, and have strong drought resistance.

[0049] 2. Take the leaves of the drought-treated Caragana plant obtained in step 1, first extract the total RNA, and then reverse transcribe to obtain the cDNA of Caragana.

[0050] 3. After completing step 2, use the cDNA of Caragana caragana as a template, and use the primer pair consisting of primer F: 5'-TCGCTTTCTCCTTAACCGTA-3' and primer R: 5'-AAACCTTAAATTGAGATACT-3' to carry out PCR amplification, and then recover A PCR am...

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Abstract

The invention discloses a specific DNA fragment com58276 and an application thereof to adjustment and control of stress resistance of plants. The nucleotide sequence of the specific DNA fragment com58276 is as shown in SEQID NO:1. The experiment verifies that the specific DNA fragment com58276 is transmitted into a wild type arabidopsis thaliana Columbia-0 subtype, and transgenic arabidopsis thaliana is obtained; and compared with the wild type arabidopsis thaliana Columbia-0 subtype, the transgenic arabidopsis thaliana has the advantage that drought resistance is improved, and improvement ofthe drought resistance is reflected in the respects of reduction of malondialdehyde content, increment of soluble sugar content, reduction of water loss rate and increment of survival rate. Therefore,it can be seen that the specific DNA fragment com58276 can adjust and control the stress resistance of the plants. The specific DNA fragment com58276 has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a specific DNA segment com58276 and its application in regulating plant stress resistance. Background technique [0002] Caragana caragana is a leguminous shrub that mainly grows in the sandy land of desert or semi-desert areas. It has a highly developed root system, strong drought tolerance and cold tolerance. Caragana caragana is not only an important pasture and industrial raw material, but also can maintain water and soil, prevent soil desertification, and has important economic and ecological value (Fang X W, Li J H, Xiong Y C, Xu D H, Fan X W, Li F M. Responses of Caraganakorshinskii Kom. to shoot removal: mechanisms underlying regrowth [J]. Ecological Research, 2007, 23(5): 863-871.). [0003] So far, the research on Caragana caragana has mainly focused on biological characteristics, physiological changes and anatomical structure, while there are very few studies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 裴新梧龙艳张天豹符娜
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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