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A kind of blueberry artificial seed preparation method

A technology for artificial seeds and blueberries, applied in the field of plant biology, can solve problems such as inability to adapt and slow reproduction, and achieve the effects of novel resources, good effects and good water absorption.

Active Publication Date: 2022-07-05
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blueberry propagation method usually adopts cutting propagation and tissue culture propagation, and the propagation speed is relatively slow, which cannot meet the needs of the fast-growing blueberry industry.

Method used

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  • A kind of blueberry artificial seed preparation method
  • A kind of blueberry artificial seed preparation method
  • A kind of blueberry artificial seed preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] On the ultra-clean workbench, put the sterile stem section with one sprout into the embedding medium (artificial endosperm, the formula is: WPM+IAA 1.0mg / L+2ip 0.05mg / L+4wt% maltose, pH 5.8) Mix well, then use a straw to suck one propagule at a time, drop it into the coagulant (artificial seed coat, the formula is: 5wt% sodium alginate + 3wt% CaCl 2 +3wt% cellulose+5wt% hemicellulose) for 5min ion exchange reaction. Then rinse with sterile water for 10 min to terminate the reaction. Pick up and dry. When transplanting and refining seedlings, the ratio of the three substrates of sawdust, soil and perlite is 5:1:1, keeping the substrate moist, the air humidity is 80%, and spraying water 4 to 5 times a day for indoor refining and transplanting.

Embodiment 2

[0024] On the ultra-clean workbench, put the sterile stem section with one bud into the embedding medium (artificial endosperm, the formula is: WPM+IAA 3.0mg / L+2ip 0.2mg / L+1wt% maltose, pH 5.8) Mix well, and then use a straw to suck one propagule at a time, drop it into the coagulant (artificial seed coat, the formula is: 1wt% sodium alginate + 1wt% CaCl 2 +1wt% cellulose+3wt% hemicellulose) for 10min ion exchange reaction. Then rinse with sterile water for 15 min to terminate the reaction. Pick up and dry. When transplanting and refining seedlings, the ratio of the three substrates of sawdust, soil and perlite is 1:1:5, keeping the substrate moist, the air humidity is 80%, and spraying water 4 to 5 times a day for indoor refining and transplanting.

Embodiment 3

[0026] On the ultra-clean bench, put the sterile stem section with one bud into the embedding medium (artificial endosperm, the formula is: WPM+IAA 2.0mg / L+2ip 0.1mg / L+7wt% maltose, pH 5.8) Mix well, then use a straw to suck one propagule at a time, drop it into the coagulant (artificial seed coat, the formula is: 3wt% sodium alginate + 2wt% CaCl 2 +5wt% cellulose+1wt% hemicellulose) for 15min ion exchange reaction. Then rinse with sterile water for 20 min to terminate the reaction. Pick up and dry. When transplanting and refining seedlings, the ratio of the three substrates of sawdust, soil and perlite is 1:1:1, keeping the substrate moist, the air humidity is 80%, and spraying water 4 to 5 times a day for indoor refining and transplanting.

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Abstract

The invention belongs to the field of plant biotechnology and discloses a preparation method of blueberry artificial seeds. On the ultra-clean workbench, put the sterile stem section with one bud into the embedding medium, mix well, and then suck one propagule at a time with a straw, drop it into the coagulant to carry out ion exchange reaction for 5-15min; Then rinse with sterile water for 10-20min to terminate the reaction; pick it up and dry it; when transplanting and refining seedlings, the ratio of three kinds of substrate chips, soil and perlite is 1-5:1-5:1-5, keeping the matrix Humid, the air humidity is 70-90%, water is sprayed 4-5 times a day, and indoor seedling transplanting is carried out. Based on modern plant tissue culture technology, establish a "top" artificial seed technology system for blueberry elite varieties with the advantages of stable system, high seedling emergence rate, simplicity and practicality, and lay the foundation for the rapid propagation of blueberry seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant biology, and relates to a preparation method of blueberry artificial seeds. Background technique [0002] Plant artificial seeds (Artificial seeds) or synthetic seeds (Synthetic seeds) are artificial seeds. Artificial seed technology is a new technology developed on the basis of plant in vitro culture technology. Artificial seeds are artificial capsules containing propagules such as plant embryoid bodies or buds, nutrients, hormones and other components. The concept of artificial seeds was first proposed by Murashige at the 4th International Congress of Plant Tissue and Cell Culture in 1978. Murashige believes that with the continuous development of plant tissue culture technology, a large number of embryoid bodies can be simultaneously cultivated with a small number of explants. These embryoid bodies are embedded in a certain capsule to make them function as seeds, which can be directly used Sow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/23A01G24/28A01G24/15
CPCA01H4/006A01H4/001A01G31/00A01G24/23A01G24/28A01G24/15
Inventor 侯义龙蔡军
Owner DALIAN UNIV