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Red maple artificial seed production method

A technology for artificial seeds and production methods, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve problems such as unfavorable management, large variability, uneven emergence, etc., and achieves simple production, good stability, and seedling emergence. high rate effect

Active Publication Date: 2020-02-11
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest feature of Chinese red maple is that the color changes from normal green to red in autumn, "October frost leaves are red like fire", "frost leaves are red like February flowers", but most of China red maple are sown seedlings, and the variability is relatively large. Large, uneven emergence, large differences in growth of offspring, not conducive to management, and affect its ornamental and medicinal value

Method used

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  • Red maple artificial seed production method
  • Red maple artificial seed production method
  • Red maple artificial seed production method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The screening of embodiment 1 artificial endosperm

[0017] MS, 1 / 2MS, 1 / 4MS, 1 / 8MS are the basic nutrients of artificial endosperm, and IAA, ZT, GA and maltose are added as auxiliary nutrients to promote the germination and growth of somatic embryos. Set the concentration of IAA: 0.5-4.0 mg / L; the concentration of ZT: 0.1-2.0 mg / L; the concentration of GA: 0.5-2.0 mg / L; the concentration of maltose: 1.0-7.0%. Orthogonal design of 5 factors and 4 levels was carried out, and the artificial endosperm formula was screened through orthogonal experiment without considering the interaction. The screening index is based on the rooting rate of somatic embryos (see Table 1-1).

[0018] Experimental design: In the artificial endosperm, the basic medium is MS (A1), 1 / 2MS (A2), 1 / 4MS (A3), 1 / 8MS (A4); the concentration of IAA is designed to be 0.5mg / L (B1), 1.0 mg / L(B2), 2.0mg / L(B3) and 4.0mg / L(B4); ZT concentrations are designed to be 0.1mg / L(C1), 0.5mg / L(C2), 1.0mg / L(C3) and 2...

Embodiment 2

[0043] The screening of embodiment 2 artificial seed coats

[0044] The Chinese maple somatic embryo was used as the propagule, 1 / 4MS+IAA2.0mg / L+ZT0.1mg / L+GA1.0mg / L+maltose 7% was used as the artificial endosperm, and the suitable artificial seed coat was screened. The artificial seed coat is made of sodium alginate with a concentration of 1% to 5%, and CaCl with a concentration of 1% to 3%. 2 , the cellulose with a concentration of 1% to 5% and the hemicellulose solution with a concentration of 1% to 5% cooperate with each other. Orthogonal design was made with 4 factors and 3 levels, and the artificial seed coat was screened through orthogonal test without considering the interaction. The screening index is based on the germination rate of the artificial seeds on the 1 / 4MS+7% maltose medium (see Table 2-1).

[0045] Experimental design: In the artificial seed coat, the concentration of sodium alginate is 1% (A1), 3% (A2), 5% (A3); CaCl 2 The concentration is 1% (B1), 2% (...

Embodiment 3

[0063] The screening of embodiment 3 transplanting substrate

[0064] Three kinds of substrates, wood chips, humus and perlite, are used in different proportions (1~5:1~5:1~5). Transplant and harden the seedlings, keep the substrate moist, the air humidity is 70% to 90%, spray water 4 to 5 times a day, and carry out indoor hardening and transplanting.

[0065] Experimental design: In the transplanting matrix, three kinds of ratios were adopted for the three kinds of substrates: wood chips: humus: perlite = 5:1:1, wood chips: humus : perlite = 1:4:1 and wood chips: humus: perlite = 1:1:5. When examining above-mentioned three kinds of proportioning, the survival rate of artificial seed (seeing table 3-1). 10 seeds were transplanted per pot for each treatment, and 5 repetitions were set.

[0066] Table 3-1 Survival rate of transplanting on different proportioning substrates (%)

[0067]

[0068] Table 3-2 Analysis of Variance for Table 3-1

[0069] source of var...

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Abstract

The invention discloses a red maple artificial seed production method, and belongs to the technical field of artificial seeds. The production method includes the steps: firstly, placing sterile Chinese red maple somatic embryos into artificial endosperms on a super-clean bench and uniformly mixing the sterile Chinese red maple somatic embryos and the artificial endosperms; secondly, sucking one propagule by a sucker every time, dropping the propagule into an artificial seed coat to perform ion exchange reaction for 15 minutes, rinsing a reactant by sterile water for 20 minutes, terminating reaction, and fishing up and drying the reactant in the air to obtain red maple artificial seeds; finally, performing transplanting and acclimatization by a transplanting medium, keeping the medium wet at the air humidity of 70%-90%, spraying water 4-5 times a day and performing indoor acclimatization and transplanting. The produced red maple artificial seeds are good in system stability, simple andconvenient to produce and high in emergence rate, the Chinese red maple somatic embryos are used as the propagules, and used resources are novel.

Description

technical field [0001] The invention belongs to the technical field of artificial seeds, and in particular relates to a method for preparing red maple seeds. Background technique [0002] Plant artificial seeds (Artificialseeds) or synthetic seeds (Syntheticseeds) are artificially manufactured seeds. Artificial seed technology is a new technology developed on the basis of plant in vitro culture technology. Artificial seeds are artificial capsules containing propagules such as plant embryoids or buds, nutrients, hormones and other ingredients. The concept of artificial seeds was first proposed by Murashige at the 4th International Plant Tissue and Cell Culture Conference in 1978. Murashige believes that with the continuous development of plant tissue culture technology, many embryoid bodies can be simultaneously cultured with a small amount of explants. These embryoid bodies are embedded in some kind of capsule to make them have the function of seeds, and can be used direct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/23A01G24/28A01G24/15
CPCA01H4/006A01H4/001A01G31/00A01G24/23A01G24/28A01G24/15
Inventor 侯义龙蔡军
Owner DALIAN UNIV