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A kind of preparation method of clove artificial seeds

A production method and technology of artificial seeds, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve the problems of affecting ornamental value, large variation of offspring, unfavorable management, etc., and achieve good stability, novel resources, and production easy effect

Active Publication Date: 2022-08-05
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cloves are mainly propagated by seed sowing, and the offspring vary greatly, which is not conducive to management and also affects its ornamental value

Method used

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  • A kind of preparation method of clove artificial seeds
  • A kind of preparation method of clove artificial seeds
  • A kind of preparation method of clove artificial seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Screening of artificial endosperm

[0016] First, the optimum rooting medium was screened, and the agar was removed from the optimum rooting medium as artificial endosperm. With MS, 1 / 2MS, 1 / 4MS, and 1 / 8MS as the basic medium, the concentration of 6-BA is set as: 0.3-1.0mg / L; the concentration of NAA is: 0.5-2.0mg / L; the concentration of banana puree is: 0.5-4.0%; activated carbon concentration: 0.5-4%; sucrose concentration is 3%, agar concentration is 7%. An orthogonal design with 5 factors and 4 levels was used to screen the artificial endosperm formula by orthogonal test without considering the interaction. The rooting rate of stem segments was used as the screening index (see Table 1-1).

[0017] Experimental design: In artificial endosperm, the basic medium is MS(A1), 1 / 2MS(A2), 1 / 4MS(A3), 1 / 8MS(A4); 6-BA concentration is designed to be 0.3mg / L(B1) , 0.5mg / L(B2), 0.8mg / L(B3) and 1.0mg / L(B4); NAA concentrations were designed as 0.5%mg / L(C1), 1.0%mg / L(C...

Embodiment 2

[0042] Example 2 Screening of artificial seed coats

[0043] Taking clove aseptic tissue culture seedlings with sprouts and stems as propagules, 1 / 2MS+6-BA0.5mg / L+NAA 1.5mg / L+sucrose 3.0%+banana puree 4%+activated carbon 2% as artificial endosperm. Screening of artificial seed coats. The artificial seed coat is made of sodium alginate with a concentration of 1% to 5% and CaCl with a concentration of 1% to 3%. 2 , The cellulose with a concentration of 1% to 5% and the hemicellulose solution with a concentration of 1% to 5% are matched with each other. A 4-factor and 3-level orthogonal design was used to screen artificial seed coats without considering the interaction. The germination rate of artificial seeds on 1 / 2MS+banana puree 4% medium was used as the screening index (see Table 2-1).

[0044] Experimental design: In artificial seed coat, the concentration of sodium alginate is 1% (A1), 3% (A2), 5% (A3); CaCl 2 Concentrations are 1% (B1), 2% (B2), 3% (B3); cellulose conc...

Embodiment 3

[0062] Example 3 Screening of transplanted substrates

[0063] Use three kinds of substrates, including wood chips, humus, and perlite, to transplant and refine seedlings, keep the substrate moist, and the air humidity is 70% to 90%. Treatments were transplanted with 10 seeds per pot, and 5 replicates were set. The suitable substrate was screened according to the transplanting survival rate (see Table 3-1).

[0064] Table 3-1 Transplanting survival rate (%) on different substrates

[0065]

[0066] The variance analysis of Table 3-2 to Table 3-1

[0067] source of variation degrees of freedom sum of square mean square F value F0.05 F0.01 processing room 2 2333.33 1166.67 38.89** 3.89 6.93 error 12 360.00 30.00 total variation 14 2693.33

[0068] Table 3-3 is a significant difference test for the data in Table 3-1

[0069] deal with average 5% difference 1% difference Process 2 76.00 a...

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Abstract

A method of making a clove artificial seeds belongs to the field of plant artificial seed production technology.This production method is first placed on the hydrococcipitigation seedlings on the ultra -net workbench, put the sprout segment of the sprouts of the aged stems in the artificial embryo, mix well, and then use a straw to draw a reproduction each time.The exchange reaction was 15 minutes, and then drifted with sterile water for 20min, terminated the reaction, picked up and dried, and finally used the transplanting matrix to keep the substrate moist. The air humidity was 70 % to 90 %.Second, indoor seedlings are transplanted.The lilac artificial seed technology system established by the present invention is good, the production is simple, and the seedling rate is high. At the same time, the lilac used by the present invention is my country's precious ornamental tree species, novel resources, and the first time its artificial seed technology system is established.

Description

technical field [0001] The invention belongs to the technical field of plant artificial seed production, and in particular relates to a preparation method of clove artificial seeds. Background technique [0002] Plant artificial seeds (Artificial seeds) or synthetic seeds (Synthetic seeds) are artificial seeds. Artificial seed technology is a new technology developed on the basis of plant in vitro culture technology. Artificial seeds are artificial capsules containing propagules such as plant embryoid bodies or buds, nutrients, hormones and other components. The concept of artificial seeds was first proposed by Murashige at the 4th International Congress of Plant Tissue and Cell Culture in 1978. Murashige believes that with the continuous development of plant tissue culture technology, a large number of embryoid bodies can be simultaneously cultivated with a small number of explants. These embryoid bodies are embedded in a certain capsule to make them function as seeds, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/23A01G24/28A01G24/15
CPCA01H4/006A01H4/001A01G31/00A01G24/23A01G24/28A01G24/15
Inventor 侯义龙蔡军
Owner DALIAN UNIV