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Blueberry artificial seed preparation method

An artificial seed and blueberry technology, applied in the field of plant biology, can solve problems such as inability to adapt and slow reproduction speed, and achieve the effect of novel resources, good effect and high emergence rate

Active Publication Date: 2020-02-11
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blueberry propagation method usually adopts cutting propagation and tissue culture propagation, and the propagation speed is relatively slow, which cannot meet the needs of the fast-growing blueberry industry.

Method used

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  • Blueberry artificial seed preparation method
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  • Blueberry artificial seed preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] On the ultra-clean workbench, put the aseptic stem with a bud into the embedding agent (artificial endosperm, the formula is: WPM+IAA 1.0mg / L+2ip 0.05mg / L+4wt% maltose, pH 5.8) Mix well, and then use a straw to suck one propagule at a time, and drop it on the coagulant (artificial seed coat, the formula is: 5wt% sodium alginate + 3wt% CaCl 2 +3wt% cellulose+5wt% hemicellulose) for 5min ion exchange reaction. Then rinse with sterile water for 10 minutes to terminate the reaction. Pick up and dry. When transplanting and smelting seedlings, the ratio of the three substrates, wood chips, soil, and perlite is 5:1:1. Keep the substrate moist, with an air humidity of 80%, spray water 4 to 5 times a day, and transplant the indoor seedlings.

Embodiment 2

[0024] On the ultra-clean workbench, put the aseptic stem with a bud into the embedding agent (artificial endosperm, the formula is: WPM+IAA 3.0mg / L+2ip 0.2mg / L+1wt% maltose, pH 5.8) Mix well, and then use a straw to suck up one propagule at a time, and drop it on the coagulant (artificial seed coat, the formula is: 1wt% sodium alginate + 1wt% CaCl 2 +1wt% cellulose+3wt% hemicellulose) in the ion exchange reaction for 10 minutes. Then rinse with sterile water for 15 minutes to terminate the reaction. Pick up and dry. When transplanting and smelting seedlings, the ratio of the three substrate chips, soil, and perlite is 1:1:5. Keep the substrate moist, with an air humidity of 80%, spray water 4 to 5 times a day for indoor smelting and transplanting.

Embodiment 3

[0026] On the ultra-clean workbench, put the aseptic stem with a bud into the embedding agent (artificial endosperm, the formula is: WPM+IAA 2.0mg / L+2ip 0.1mg / L+7wt% maltose, pH 5.8) Mix well, and then use a straw to suck one propagule at a time, and drop it on the coagulant (artificial seed coat, the formula is: 3wt% sodium alginate + 2wt% CaCl 2 +5wt% cellulose+1wt% hemicellulose) in the ion exchange reaction for 15 minutes. Then rinse with sterile water for 20 minutes to terminate the reaction. Pick up and dry. When transplanting seedlings, the ratio of the three substrate chips, soil, and perlite is 1:1:1. Keep the substrate moist, with an air humidity of 80%, spray water 4 to 5 times a day, and transplant the indoor seedlings.

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Abstract

The invention belongs to the technical field of plant biology, and discloses a blueberry artificial seed preparation method. On a super-clean bench, a sterile stem with one bud is placed into embedding agents and uniformly mixed, and one propagule is sucked by a sucker every time and dropped into coagulating agents to perform ion exchange reaction for 5-15 minutes. A reactant is rinsed by sterilewater for 10-20 minutes, reaction is terminated, and the reactant is fished up and dried in the air. During transplantation and acclimatization, the ratio of wood chips, soil and perlite serving as media is (1-5):(1-5):(1-5), the media are kept wet at the air humidity of 70-90%, water is sprayed 4-5 times a day, and indoor acclimatization and transplanting are performed. Based on a modern plant tissue culture technology, a blueberry excellent variety 'top-level' artificial seed technology system with the advantages of system stability, high emergence rate, simplicity, convenience, practicability and the like is built, and a foundation is laid for rapid propagation of blueberry seedlings.

Description

Technical field [0001] The invention belongs to the technical field of plant biology, and relates to a method for preparing blueberry artificial seeds. Background technique [0002] Artificial seeds or Synthetic seeds are artificial seeds. Artificial seed technology is an emerging technology developed on the basis of plant in vitro culture technology. Artificial seed is an artificial capsule containing plant embryoid or bud and other propagules, nutrients, hormones and other ingredients. The concept of artificial seeds was first proposed by Murashige at the 4th International Plant Tissue Cell Culture Conference in 1978. Murashige believes that with the continuous development of plant tissue culture technology, a small number of explants can be used to simultaneously cultivate a large number of embryoid bodies. These embryoid bodies are embedded in some kind of capsule to make them have the function of seeds and can be used directly. Sow in the field. In 1985, the Japanese sch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/23A01G24/28A01G24/15
CPCA01H4/006A01H4/001A01G31/00A01G24/23A01G24/28A01G24/15
Inventor 侯义龙蔡军
Owner DALIAN UNIV