Sealed microwell assay

A micropore and bead technology, applied in the field of micropore analysis assays, can solve the problems of difficult pairing, accuracy, ease of use and efficiency to be improved, etc., to increase the concentration, improve the ligand binding efficiency, and improve the accuracy. Effects of Sex and Efficiency
CN110799654APending Publication Date: 2020-02-14MUWELLS INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MUWELLS INC
Publication Date
2020-02-14

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Abstract

The present invention provides methods, systems and kits for performing microwell analysis in sealed small volumes. The microwells of the invention are sealed through the pairing of microwell and beadgeometry wherein the diameter of the beads is greater than the diameter of the bottom of the microwells. Loading the beads into the microwells seals samples within an analytical space between the bottom of a nested bead and the bottom of the subject microwell.
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Description

[0001] Cross References to Related Applications

[0002] This application claims priority to Provisional Application Serial No. 62 / 474,283, filed March 21, 2017, which is hereby incorporated by reference in its entirety for all purposes. technical field

[0003] The present invention relates to assays for microwell analysis. More specifically, the present invention relates to assays of sealed microwells for the analysis of small volumes of biological samples. Background technique

[0004] Microwell arrays for biological assays have been in use for some time. One of the applications of microwell arrays is to determine the expression profile of individual cells. For example, U.S. Patent Application Publication No. 2016 / 0289669 by Fan et al. discloses a microwell-based device and system for single cell expression profiling, wherein prior to analysis of cell biological activity, single Cells and individual ligand-coated beads are loaded into microwells and a pellet is allowed...

Claims

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