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Immunology functions and purpose of GPR174

A kind of use, B cell technology, applied in the field of GPR174 immunological function and its use, can solve the lack of in-depth molecular mechanism and other problems

Active Publication Date: 2020-02-18
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is still a lack of in-depth molecular mechanisms for the impact of gender factors on humoral immune responses

Method used

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  • Immunology functions and purpose of GPR174
  • Immunology functions and purpose of GPR174
  • Immunology functions and purpose of GPR174

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0166] Example 1 Intrinsic Differences in Germinal Center Formation Between Male B Cells and Female B Cells

[0167] In this example, the inventors detailed the inherent differences in the size of the germinal center and the inherent difference in dynamic positioning before the formation of the germinal center response between male and female B cells.

[0168] To investigate whether there are inherent differences in the ability of male and female B cells to form germinal centers, the inventors constructed a system for the co-infusion of antigen-specific male and female B cells: the same amount of male or female antigen Specific MD4 B cells (dsRed-red fluorescent protein marker) were mixed with the same number of male antigen-specific MD4B cells (GFP-green fluorescent protein marker) and then reinfused into male recipient mice ( figure 1 a Longitudinal first column), and at the same time, a certain number of male antigen-specific OT2 T cells were reinfused as helper T cells to ...

Embodiment 2

[0170] Example 2 GPR174 regulates the dynamic localization of B cells activated by antigen stimulation and inhibits the germinal center response in male non-female mice

[0171] In this example, the inventors introduced in detail that the newly discovered G protein-coupled receptor GPR174 can regulate the dynamic localization of B cells in vivo and its influence on the germinal center response.

[0172] In order to explore why male B cells and female B cells have inherent differences in the ability to accumulate to the follicular center region before forming a germinal center reaction, the inventors first discovered that a new G protein-coupled receptor GPR174 can act as a chemotactic receptor to regulate B Dynamic localization of cells in vivo. The inventors used GFP fluorescence-labeled retroviral vectors to overexpress GPR174 to activated B cells in vitro, and then reinfused them into mice, and found that a large number of B cells overexpressing GPR174 were obviously gather...

Embodiment 3

[0175] Example 3 GPR174 is the receptor of chemokines CCL21 and CCL19 and regulates the differential chemotactic migration of male and female B cells in vitro and in vivo

[0176] In this example, the inventors introduced in detail that the newly discovered G protein-coupled receptor GPR174 is actually a chemokine receptor that recognizes chemokines CCL21 and CCL19, and explained that GPR174-CCL21 / CCL19 mediates male Differences in in vitro chemotaxis assays and in vivo dynamic localization of B cells and female B cells.

[0177] To further explain why GPR174 affects germinal center responses in male non-female mice, the inventors first started by identifying a ligand that interacts with GPR174. Since most of the chemokines in existing studies are secreted by spleen stromal cells, the inventors prepared the supernatant of mouse spleen stromal cells cultured in vitro, and detected that it contained strong B cells that activated in vitro Ligand properties of GPR174-dependent ch...

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Abstract

The invention provides immunology functions and purpose of GPR174. The GPR174 comprises GPR174 and / or CCL21 and / or CCL19 inhibition type antibodies or antagonists or expression inhibitors or low-expression cell medicines, can be effectively used for increasing humoral immunity response of organisms particularly male organisms or treating or preventing immune deficiency, so that the level of a specific antibody produced in accordance with external antigens of an inoculator can be increased, or the level of a specific antibody produced in accordance with tumor relevant antigens of a tumor patient can be increased, and tumor immunity treatment effects can be improved.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, the present invention relates to the immunological function of GPR174 and its use, more specifically, the present invention relates to the use of GPR174 as a marker of male B cells, and the method of inhibiting the accumulation of activated B cells to the follicular central region The method, the method for promoting the aggregation of activated B cells to the center of the follicle, the use of the reagent in the preparation of kits or medicines, and the method for screening medicines. Background technique [0002] When the body is invaded by foreign pathogens, it needs to rely on the response of the germinal center in the peripheral lymphoid tissue to establish a long-lasting and high-affinity humoral immune response. Therefore, studying the immunological mechanism of the germinal center response has important guiding significance for the realization of high efficacy of clinical ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A61K45/00A61P37/00
CPCG01N33/5052A61K45/00A61P37/00
Inventor 祁海赵若竹陈欣马伟伟张晋渝
Owner TSINGHUA UNIV
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