Electrochemical adaptor biosensor based on hydrogel protection, and preparation method and application thereof
A biosensor and hydrogel technology, applied in scientific instruments, biological testing, instruments, etc., can solve problems such as false positives, poor signal stability, and false negatives, and achieve the effect of preventing interference and improving electrode stability
Pending Publication Date: 2020-02-18
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology
[0005] However, the aptamer-based electrochemical biosensor detection platform still faces major challenges in real-time and in situ detection in whole blood samples or complex environments in vivo: 1) baseline drift is prone to occur, resulting in test output Poor signal stability performance, so prone to false negative or false positive test results; 2) biocompatibility in vivo needs to be improved
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Embodiment 1
[0025] The agarose is formulated into aqueous solutions with concentrations of 0.1%, 0.25%, 0.5%, 1%, and 3% in mass percentage respectively, and the state diagram of the prepared agarose hydrogel is shown in figure 1 , figure 1 In A, from left to right, there are 0.1%, 0.25%, 0.5%, 1%, and 3% agarose hydrogels. From the two aspects of mechanical strength and stability, the water in the concentration range of 0.5% to 3% The gel is better, and the 0.1% concentration cannot form a stable hydrogel; figure 1 B is carefully and slowly adding 0.1mL blood to the formed agarose hydrogel. It can be seen that the hydrogel with a concentration range of 0.5% to 3% is better, and the blood penetration is small;
[0026] Immerse the prepared electrodes in the agarose hydrogel with a mass percent concentration of 0.5%, 1%, and 3% respectively, keep it for 2 seconds and then take it out quickly. This operation is repeated 3 times, and then the electrode is quickly frozen at -78°C taken out ...
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The invention discloses an electrochemical adaptor biosensor based on hydrogel protection, wherein the electrochemical adaptor biosensor is prepared through evenly coating a surface of an electrode with hydrogel. The invention also provides a preparation method of the electrochemical adaptor biosensor, and the method comprises the following steps: preparing hydrogel solution, immersing the electrode in the hydrogel solution, keeping for a period of time and then taking out the electrode, repeating operations, then, freezing the electrode quickly in liquid nitrogen, taking out the electrode andobtaining the electrochemical adaptor biosensor. The electrochemical adaptor biosensor provided by the invention is prepared through evenly coating the surface of the electrode with the hydrogel, when electrochemical adaptor biosensor performs in vivo blood concentration test, micro molecules can penetrate through the hydrogel, micro molecules can not penetrate through the hydrogel, thus, interference of hemoglobin can be prevented effectively, electrode stability is improved, and accurate test of in vivo blood concentration is realized further.
Description
technical field [0001] The invention relates to the technical field of drug detection, in particular to a class of electrochemical aptamer biosensors based on hydrogel protection and a preparation method and application thereof. Background technique [0002] Health is the eternal theme of human development. Especially since the 21st century, the treatment of human health and diseases in the world has entered a new stage, gradually developing from the traditional medical model to "precision medicine" and personalized medical service model. Precision medicine refers to an emerging medical model of disease prevention and treatment that takes into account differences in individual genes, environment and living habits, and is formulated for individual differences. [0003] Clinically, precision medicine and personalized medical services need to provide diagnosis and treatment services for individual patient differences. For example, when a doctor administers medicine to a patien...
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IPC IPC(8): G01N27/327G01N33/50
CPCG01N27/327G01N33/94
Inventor 李辉夏帆李少光戴俊娄筱叮朱满李红星王园园
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)