A pharmaceutical composition of gastrodin and mannuronic acid oligosaccharides and its application
A technology of uronic acid oligosaccharide and gastrodin, which is applied in the field of medicine to achieve the effects of protecting nerve cells and cardiomyocytes, preventing and treating nervous system diseases and myocardial hypoxia damage, and promoting mitochondrial regeneration
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Embodiment 1
[0022] Example 1: Combined application of gastrodin and mannuronic acid oligosaccharide significantly protects nerve cells from Aβ protein damage
[0023] Nerve cell PC12 was inoculated in complete MEM medium (containing 100U / mL penicillin, 100U / mL streptomycin and 10% FBS), cultured in a 37°C, 5% CO2 incubator, and 8000 cells per well were planted In a 96-well plate, the cell viability without adding Aβ1-42 was used as a negative control to observe the inhibitory effect of gastrodin combined with mannuronic acid oligosaccharides on neurocytotoxicity induced by Aβ.
[0024] The specific implementation steps are as follows: inoculate PC12 cells in MEM complete culture medium, culture them in 96-well plates, and incubate them in a constant temperature cell incubator for 24 hours. Gastrodin or mannuronic acid oligosaccharides were added at a concentration of 200ug / ml, and the combined group was given a compound of 1 part of gastrodin combined with 2.25 parts of mannuronic acid ol...
Embodiment 2
[0029] Example 2, the protective effect of gastrodin and mannuronic acid oligosaccharides on CoCl2 induced hypoxic injury of nerve cells and cardiomyocytes
[0030] Nerve cells PC12 or cardiomyocytes H6C2 were inoculated in MEM complete culture medium (containing 100 U / mL penicillin, 100 U / mL streptomycin and 10% FBS), and placed in a 37°C, 5% CO2 incubator for culture, and each well was 5000 cells were planted in a 96-well plate, and the cell viability without CoCl2 was used as a negative control to observe the inhibitory effect of the combined application of gastrodin and mannuronic acid oligosaccharides on the neurotoxicity induced by CoCl2.
[0031] The specific implementation steps are as follows: PC12 and H9C2 cells were inoculated in MEM or DMEM complete culture medium, cultured in a 96-well plate, placed in a constant temperature cell incubator and incubated for 24 hours, and the injury solution containing CoCl2 dissolved in advance was added, After 2 hours, add gastro...
Embodiment 3
[0037] Example 3: Combined application of gastrodin and mannuronic acid oligosaccharide significantly increases the mitochondrial membrane potential of nerve cells
[0038] Nerve cell PC12 was inoculated in complete MEM medium (containing 100U / mL penicillin, 100U / mL streptomycin and 10% FBS), cultured in a 37°C, 5% CO2 incubator, and 8000 cells per well were planted In a 96-well plate, the cell viability without Aβ1-42 was used as a negative control.
[0039] The specific implementation steps are as follows: inoculate PC12 cells in MEM complete culture medium, culture them in 96-well plates, and incubate them in a constant temperature cell incubator for 24 hours. Gastrodin or mannuronic acid oligosaccharides were added at a concentration of 200ug / ml, and the combination group was given a compound of gastrodin combined with 2.25 parts of mannuronic acid oligosaccharides at 100 or 200ug / ml, and the incubator continued to incubate for 24 hours. After the end, the mitochondrial m...
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